165,385 results

1. pAG1186 Lyn11-ECFP-FIREmate

   Plasmid

   #205168

   Purpose

   Expresses Lyn11-ECFP-FIREmate in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   Lyn11-ECFP-FIREmate
   

   Expression

   Mammalian

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFSW GFP IRES GFP

   Plasmid

   #133815

   Purpose

   Encodes EGFP that can be used as a control for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   IRES-GFP

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNL-CEF-BAFFR-CFP

   Plasmid

   #187002

   Purpose

   Expression of human BAFFR-CFP fusion protein

   Depositor

   Hermann Eibel

   Article

   Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

   Insert

   BAFFR (TNFRSF13C Human)
   

   Use

   Lentiviral

   Tags

   CFP

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-mScarlet-EFS-Blast

   Plasmid

   #227260

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFR-PATagRFP

   Plasmid

   #31950

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

   Insert

   PATagRFP
   

   Tags

   EGF receptor (EGFR)

   Expression

   Mammalian

   Mutation

   R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

   Promoter

   CMV

   Available Since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRel-Hyg

   Plasmid

   #227582

   Purpose

   Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKSI-1

   Plasmid

   #51725

   Purpose

   cloning backbone

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. XPO6-EGFP

   Plasmid

   #210371

   Purpose

   Expresses XPO6 fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   XPO6 (XPO6 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-V5-SBP-C1-HshnRNPK

   Plasmid

   #64923

   Purpose

   Expresses V5- and SBP-tagged hnRNPK in mammalian cells

   Depositor

   Elisa Izaurralde

   Insert

   hnRNPK (HNRNPK Human)
   

   Tags

   V5 and SBP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHJL3

    Plasmid

    #199378

    Purpose

    Encodes dual cistronic cytosolic jGCaMP7s and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    jGCaMP7s::SL2::mScarlet-I
    

    Expression

    Bacterial

    Mutation

    no mutations

    Available Since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

    Plasmid

    #101139

    Purpose

    Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbB8k-csg-NbGFP

    Plasmid

    #166858

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469

    Insert

    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSP2-96-merTetO-EFS-BLaR

    Plasmid

    #118713

    Purpose

    Contains an optimized 96-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDF-GFPplus

    Plasmid

    #172718

    Purpose

    Expression of a fast folding GFP variant GFP plus

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    GFP plus
    

    Expression

    Bacterial

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pArcCreER

    Plasmid

    #46389

    Depositor

    Liqun Luo

    Article

    Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

    Inserts

    Arc (Arc Mouse)
    CreERT2
    

    Use

    Mouse Targeting

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ornithine transcarbamylase (OTC)

    Plasmid

    #71877

    Purpose

    For mitochondrial unfolded protein response research

    Depositor

    Nicholas Hoogenraad

    Article

    Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

    Insert

    OTC (Otc Rat)
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

    Plasmid

    #87306

    Purpose

    An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre-dependent mouse codon-optimized Flp recombinase.
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX_EFS-Cre_ppt-del

    Plasmid

    #162073

    Purpose

    Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Insert

    Cre
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EFS (part of insert)

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

    Plasmid

    #169912

    Purpose

    Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    dCjCas9
    

    Use

    CRISPR

    Tags

    10XGCN4, 2xSV40NLS, and SV40NLS-HA

    Expression

    Mammalian

    Mutation

    D8A, H559A in cjCas9

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHS2 (hANGPTL4-V5)

    Plasmid

    #102446

    Purpose

    Expresses V5-tagged human ANGPTL4 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

    Insert

    ANGPTL4 (ANGPTL4 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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