60,522 results
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Plasmid#154104PurposeMammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable SinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-ARF
Plasmid#89123Purposemammalian expression of p14-hARF with N-terminal FLAG tagDepositorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX
Plasmid#167583PurposeExpress the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cellsDepositorInsertATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
ExpressionMammalianPromoterCMVAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pITGA4-GFP
Plasmid#81178PurposeMammalian expression of human full length ITGA4-EGFPDepositorInsertITGA4 (ITGA4 Human)
TagsEGFPExpressionMammalianMutationContains KLGFFKR motif; A1002L (please see deposi…PromoterCMVAvailable SinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP
Plasmid#132393PurposeExchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 siteDepositorInserthuman TauP301L 2N4R-EGFP (MAPT Human, Synthetic)
UseCRISPR and Cre/LoxTagsEGFPExpressionMammalianMutationProline 301 to Leucine (P301L)PromoterTREtightAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2043
Plasmid#142316PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
NEK7_HUMAN_D0
Plasmid#79735PurposeThis plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Blast-DNTGFBR2-HA
Plasmid#130888PurposeExpresses dominant negative mutant human TGFBR2DepositorInsertTGFBR2 (TGFBR2 Human)
TagsHA-tagExpressionMammalianMutationDominant Negative receptor lacking cytosolic cata…PromoterCMVAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGfa2-nLac
Plasmid#53126PurposeExpression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.DepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains -2163 to +47 (the human gfa2 segment), w…Promoterhuman GFAPAvailable SinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
Plasmid#63729PurposeTo express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).TagsT2AExpressionMammalianMutationcodon optimizedPromoterSFFVAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_IDH1_p.R132H
Plasmid#81686PurposeGateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR
Plasmid#157744Purposeknock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expressionDepositorInsertchlorotoxin containing chimeric antigen receptor targeting glioblastoma
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
UbcH5c S22R pET28a
Plasmid#12644DepositorInsertUbch5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
ExpressionBacterialAvailable SinceJuly 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFastbac1-Flag-FANCD2
Plasmid#134904Purposecodon optimized expression and purification of human FANCD2 in insect cells using baculovirusDepositorInsertFANCD2 (FANCD2 Human)
TagsFlagExpressionInsectMutationfully synthetic, codon optimized for insect expre…PromoterpolyhedronAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RIMS2_WT
Plasmid#81803PurposeGateway Donor vector containing RIMS2 ,part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SETDB1_WT
Plasmid#82291PurposeGateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SAMHD1_WT
Plasmid#81899PurposeGateway Donor vector containing SAMHD1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF1072
Plasmid#141790PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(CTLA4)
Plasmid#177905PurposeBacterial expression of his10-tagged human CTLA4 Intracellular DomainDepositorInsertCTLA4 (aa 183-223 only) (CTLA4 Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available SinceMay 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEP4 E02S EN2L
Plasmid#20922Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28DepositorArticleAvailable SinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits only