Addgene

We narrowed to 9,510 results for: Gnas

Showing: 2001 - 2050 of 9510 results

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pCMX/cyclinB1-GFP (27)

Plasmid

#26061

Depositor

Jonathon Pines

Article

Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

Insert

Cyclin B1 (CCNB1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CaNAR2

Plasmid

#64728

Purpose

FRET-based biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2

Use

Tags

Cerulean3 and YPet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pHA-eIF4E

Plasmid

#17343

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

eIF4E (EIF4E Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pRK7-Rheb

Plasmid

#15888

Depositor

John Blenis

Article

Tee et al Curr Biol. 2003 Aug 5. 13(15):1259-68.

Insert

Rheb (RHEB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
ASAP2f

Plasmid

#83960

Purpose

Fluorescent reporter for voltage imaging

Depositor

Michael Lin

Article

Yang et al Cell. 2016 Jun 30;166(1):245-57. doi: 10

Insert

ASAP2f

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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opto-RhoA-mCherry_pcDNA3.1

Plasmid

#164472

Purpose

Expresses opto-RhoA (RhoA-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

Insert

opto-RhoA

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRIP3-mCherry

Plasmid

#61386

Purpose

Expresses RIP3-mCherry in mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

RIP3 (RIPK3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pNTI819

Plasmid

#216628

Purpose

Cal-ID (V5) for mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIREVOL)-sfGFP

Plasmid

#189675

Purpose

titratable expression of sfGFP in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

sfGFP

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Rnd3

Plasmid

#23229

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Rnd3 (RND3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 Rheb1 shRNA #1

Plasmid

#26625

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Rheb1 shRNA (RHEB Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pDEST mpeg1:tomato-CAAX

Plasmid

#135215

Purpose

Red tagged macrophages

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

mpeg1 and tdTomato

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pUASattB-ERK-SPARK

Plasmid

#106923

Purpose

Express ERK-SPARK, an activity reporter of ERK in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

ERK-SPARK

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PM-Ras-LOCKR-S

Plasmid

#216498

Purpose

FRET-based reporter for Ras activity, plasma membrane localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

PM-Ras-LOCKR-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rnd1

Plasmid

#23227

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Rnd1 (RND1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pSJ111

Plasmid

#78707

Purpose

Modular vector for constitutive secreted amylase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

amyEopt

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pveg

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST Rheb1

Plasmid

#19310

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RHEB Ras homolog enriched in brain (RHEB Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pLKO.1 Rheb1 shRNA #2

Plasmid

#26626

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Rheb1 shRNA (RHEB Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only
pRc/CMV HA TrkB

Plasmid

#39978

Depositor

Yves-Alain Barde

Article

Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Insert

TrkB (Ntrk2 Rat)

Use

Tags

BM40 signal sequence and HA

Expression

Mammalian

Mutation

endogenous signal sequence was replaced with BM40…

Promoter

CMV

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-TRAF2

Plasmid

#111202

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

TRAF2 (TRAF2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoFH human RASSF1A

Plasmid

#37016

Depositor

Yutaka Hata

Article

Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

Insert

rassf1A (RASSF1 Human)

Use

Tags

FLAG and His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Ras-LOCKR-S

Plasmid

#216496

Purpose

FRET-based reporter for Ras activity, untargeted

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Ras-LOCKR-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Wnt5B

Plasmid

#35912

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5B (WNT5B Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagC wt

Plasmid

#19304

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding C (RRAGC Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pPUR-hU6-sgRNA-Sirius-8XMS2

Plasmid

#121942

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Wnt7B

Plasmid

#35915

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7B (Wnt7b Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
LgBit-NbALFA

Plasmid

#201484

Purpose

NanoBit detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

LgBit-NbALFA

Use

Tags

LgBit

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-14-3-3 epsilon

Plasmid

#116886

Purpose

Expresses HA-tagged human 14-3-3 epsilon in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 epsilon (YWHAE Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLH-sgRNA-Sirius-8XMS2

Plasmid

#121939

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rab5CA (Q79L)

Plasmid

#35140

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Constitutively Active- Q79L

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_neomycin

Plasmid

#65306

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Use

Tags

KDEL motif and Signal Sequence

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5B-TGFbeta receptor II wt

Plasmid

#11766

Depositor

Jeff Wrana, Joan Massague

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

TGFbeta receptor II (TGFBR2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EGFP-PIP5K1B

Plasmid

#202722

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1B (PIP5K1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p5E mpeg1

Plasmid

#135199

Purpose

Macrophage specific label

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

mpeg1 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

mpeg1

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA GST RagB 99L

Plasmid

#19303

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Q99L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPORT-RREB1

Plasmid

#41145

Depositor

Joshua Mendell

Article

Kent et al Oncogene. 2012 Jul 2. doi: 10.1038/onc.2

Insert

RREB-1 (RREB1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
ST-GFP

Plasmid

#162500

Purpose

Expresses ST6GAL1 with a GFP tag in its C-terminus in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

ST6Gal1 (ST6GAL1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPUR-mU6-sgRNA-Sirius-8XPP7

Plasmid

#121943

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

mouse U6 promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
vYM140_PB-pEFa1-iaaM(P.s)-Hygro

Plasmid

#160043

Purpose

Expressing iaaM for converting L-Trp to IAM

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Insert

iaaM (iaaM )

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NbV5-mCherry

Plasmid

#201474

Purpose

Indirect microscopy/imaging of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FKBP-EGFP-HOTag3

Plasmid

#106924

Purpose

Expresses FKBP-EGFP-HOTag3 in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

FKBP-EGFP-HOTag3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTiger-OptoSOS

Plasmid

#86439

Purpose

Single-construct blue light optogenetic Ras activator for use in Drosophila

Depositor

Jared Toettcher

Article

Johnson et al Dev Cell. 2017 Jan 23;40(2):185-192. doi

Insert

OptoSOS

Use

Tags

tagRFP

Expression

Insect

Mutation

Promoter

Gal4-UAS

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
625 pTol2-tp1bglob:egfp

Plasmid

#73586

Purpose

Tol2 vector with Notch responsive tp1 element driving expression of EGFP

Depositor

Nathan Lawson

Article

Quillien et al Development. 2014 Apr;141(7):1544-52. do

Insert

tp1 element driving EGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-TO-dCas9-P2A-HSA

Plasmid

#121936

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

dCas9-P2A-HSA

Use

CRISPR and Lentiviral

Tags

HSA

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI HA NEDD4 DD

Plasmid

#26999

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4 (NEDD4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C867A

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
iRFP670-PIP5K1A

Plasmid

#202749

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP670:PIP5K1A (PIP5K1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Wnt1

Plasmid

#35905

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rab5DN(S34N)

Plasmid

#35141

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Dominant Negative- S34N

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only
Human RNF144A (16-228)

Plasmid

#110752

Purpose

Bacterial Expression of RNF144A RBR domain

Depositor

David Komander

Article

Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

Insert

RNF144A (RNF144A Human)

Use

Tags

N-His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NbV5-LgBit

Plasmid

#201475

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-LgBit

Use

Tags

LgBit

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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