We narrowed to 9,252 results for: SUB
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Plasmid#198347PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 σ3A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 σ3A
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-AP-3 σ3B
Plasmid#198348PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 σ3B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 σ3B
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVRC-N-Venus-V150A-CC-Di
Plasmid#190707PurposeEncodes a modified version (V150A) of N-Venus followed by the parallel coiled-coil CC-Di.DepositorInsertN-Venus-V150A-CC-Di
UseTagsExpressionMutationV150APromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVRC-N-Venus-V150A
Plasmid#190708PurposeControl plasmid encoding a modified version (V150A) of N-Venus, but without a coiled-coil to drive reconstitution.DepositorInsertN-Venus-V150A
UseTagsExpressionMutationV150APromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
RCAS myr-c-P3k
Plasmid#196373PurposeOncogenic with addition of a myristylation signal and expresses in avian cellsDepositorInsertMyr-c-P3k-FLAG
UseRetroviral; AvianTagsflag and myristoylationExpressionMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7PI-Sce ESARC D326A
Plasmid#195609PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationT3593C, A3601C, T3602GPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgKcna2
Plasmid#192796PurposeEncodes SaCas9 and an sgRNA targeting mouse Kcna2DepositorInsertsgKcna2
UseAAV and CRISPRTagsExpressionMutationPromoterU6Available sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgKcna6
Plasmid#192797PurposeEncodes SaCas9 and an sgRNA targeting mouse Kcna6DepositorInsertsgKcna6
UseAAV and CRISPRTagsExpressionMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_rSERT_X5C_S277C
Plasmid#190177PurposeFor mammalian cell expression of rat SERT and measurement of cytoplasmic pathway accessibilityDepositorInsertrat serotonin transporter (Slc6a4 Rat)
UseTagsFLAG, His6, and c-mycExpressionMammalianMutationCys15Ala, Cys21Ala, Cys109Ala, Ser277Cys, Cys357I…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND6-L1397-N_Q1310A_right
Plasmid#187849Purposeexpresses the right-side half ND6-Q1310A in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ND6-L1397-N_Q1310A_left
Plasmid#187848Purposeexpresses the left-side half ND6-Q1310A in mammalian cellsDepositorInsertSOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N(Q1310A)–UGI–SOD2 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI
Plasmid#192113PurposeExpresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-NTD-BA.4/5 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …PromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
KEE
Plasmid#187419PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationPromoterPH and p10Available sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-66%GC-40bp
Plasmid#169200PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert66%GC-40bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-75%GC-20bp
Plasmid#169201PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert75%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-75%GC-40bp
Plasmid#169202PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert75%GC-40bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-100%GC-20bp
Plasmid#169203PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert100%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-Left-half-120bp
Plasmid#169204PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-Left-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-Right-half-120bp
Plasmid#169205PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-Right-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50-0-50%GC-Left-half-120bp
Plasmid#169206PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50-0-50%GC-Left-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50-0-50%GC-Right-half-120bp
Plasmid#169207Purpose50-0-50%GC-Right-half-120bp-hairpinDepositorInsert50-0-50%GC-Right-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-20bp
Plasmid#169196PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-40bp
Plasmid#169197PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-40bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-40bp-Revbindsite
Plasmid#169198PurposeForce-activating hairpin for 5'-to-3' helicaseDepositorInsert50%GC-40bp-hairpin-binding-site-reversed
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-66%GC-20bp
Plasmid#169199PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert66%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Xalb_I-F1_array_GG
Plasmid#178770PurposeGolden Gate vector to generate X. albilineans type I-F1 single arraysDepositorInsertGolden Gate vector BaeI recognition site to generate X. albilineans type I-F1 single arrays
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sth_I-E array_GFP dropout
Plasmid#178775PurposeGolden Gate GFP dropout vector to generate S. thermophilus type I-E single arraysDepositorInsertgfp dropout to generate S. thermophilus type I-E single arrays
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEh_gRNA1
Plasmid#178758PurposeExpression of a gRNA that targets next to PAM library, used for E. haloalkaliphila type I-E systemDepositorInsertgRNA that targets next to PAM library, used for E. haloalkaliphila type I-E system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMs_gRNA1
Plasmid#178764PurposeExpression of a gRNA that targets next to PAM library, used for Marinomonas sp. type I-E systemDepositorInsertgRNA that targets next to PAM library, used for Marinomonas sp. type I-E system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Xalb_I-C_array_GG
Plasmid#178767PurposeGolden Gate vector to generate X. albilineans type I-C single arraysDepositorInsertGolden Gate vector BaeI recognition site to generate X. albilineans type I-C single arrays
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc3_gRNA1
Plasmid#178746PurposeExpression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 systemDepositorInsertgRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLm_gRNA1
Plasmid#178752PurposeExpression of a gRNA that targets next to PAM library, used for L. mobilis type I-E systemDepositorInserta gRNA that targets next to PAM library, used for L. mobilis type I-E system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc2_gRNA1
Plasmid#178740PurposeExpression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 systemDepositorInsertgRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-td-sfGFP
Plasmid#180150Purposetd-sfGFP cre reporterDepositorInserttd-sfGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCm No carrier NikJC199U
Plasmid#174361PurposeExpression plasmid with a TEV removable C-term 8xHis Tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEL2298
Plasmid#164603PurposeExpression and Complementation plasmid for MlaD (A17F, A20F, V24F)DepositorInsertMlaFEDCB
UseTagsExpressionBacterialMutationMlaF-(6xHis-MlaE)-MlaD(Ala17Phe, Ala20Phe, Val24P…PromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E
Plasmid#125797PurposeHistone H2B-superfolder GFP and tdTomato fused by unskippable linkerDepositorInsertH2BsfGFP-tdTomato
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS10_K139R
Plasmid#127137DepositorInsertRPS10 (RPS10 Human)
UseLentiviralTagsExpressionMutationK139RPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS10_K138R
Plasmid#127136DepositorInsertRPS10 (RPS10 Human)
UseLentiviralTagsExpressionMutationK138RPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISH-Vmn2r76
Plasmid#108084PurposePreparation of RNA probe for ISH of mouse Vmn2r76 geneDepositorInsertVmn2r76 (Vmn2r76 Mouse)
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGRNA-LbCpf1-SpCas9
Plasmid#119673PurposeEmpty vector for cloning LbCpf1-SpCas9 fgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGRNA-AsCpf1-SpCas9
Plasmid#119674PurposeEmpty vector for cloning AsCpf1-SpCas9 fgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGRNA-LbCpf1-spCas9-MS2
Plasmid#119675PurposeEmpty vector for cloning LbCpf1-spCas9 MS2-fgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOEM1_pCMV:axFgf8-pPH:vsvged
Plasmid#111159PurposeBaculovirus rescue vector, VSVGED pseudotype, hShhDepositorInsertaxFgf8
UseBaculoviral rescue shuttle vectorTagsExpressionInsect and MammalianMutationwtPromoterCMVAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS2-1-Brr2
Plasmid#99995PurposeExpress yeast Brr2 with Y2H binding domain fusionDepositorInsertBrr2 (BRR2 Budding Yeast)
UseTagsY2H binding domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Rcl1
Plasmid#99992PurposeExpress yeast Rcl1 with Y2H activation domain fusionDepositorInsertRcl1 (RCL1 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationContains amino acids 1-217 of Rcl1PromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Kre33
Plasmid#99990PurposeExpress yeast Kre33 with Y2H activation domain fusionDepositorInsertKre33 (KRE33 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-1-Kre33
Plasmid#99989PurposeExpress yeast Kre33 with Y2H binding domain fusionDepositorInsertKre33 (KRE33 Budding Yeast)
UseTagsY2H binding domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2 3XFLAG-EGFP-NLS
Plasmid#87778PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-HygroDepositorInserteGFP
UseTags3xFLAG and NLSExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS
Plasmid#87777PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-HygroDepositorInsertMKK7-EE
UseTags3xFLAG and NLSExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only