161,799 results

1. DENV2 NS2B-NS3 protease: Co-expression of DENV-2 NS2B and NS3

   Plasmid

   #204808

   Purpose

   Bacterial co-expression of Dengue2 NS2B and NS3 proteins

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Dengue2 NS2B (POLY Dengue2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pAAV-FAH-rtTA3G

   Plasmid

   #120309

   Purpose

   An AAV vector that expresses rtTA3G under the c-fos promoter

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   rtTA3G
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   c-fos

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. rAAV2/MG1.1

   Plasmid

   #184540

   Purpose

   AAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsid

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

   Insert

   AAV-MG1.1 capsid
   

   Use

   AAV; Helper vector for packaging

   Tags

   Expression

   Mutation

   changes on AAV9 capsid: K449R, A587L, Q588M; inse…

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGP-CMV-jGCaMP7s

   Plasmid

   #104463

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7s
   

   Use

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2

   Plasmid

   #202566

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopy

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Use

   Tags

   mNeonGreen2 on ORF1p

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-HA-RIPK1

   Plasmid

   #78834

   Purpose

   To overexpress RIPK1 in Mammalian Cell

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

   Plasmid

   #221899

   Purpose

   Expresses alpha tubulin fused to mStayGold

   Depositor

   Janelia Integrative Imaging

   Insert

   mStayGold-aTubulin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLP-PIGA (pLP140)

   Plasmid

   #168461

   Purpose

   Big-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

   Depositor

   Matthew Maurano, Jef Boeke

   Article

   Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmyc-TNRC6B

   Plasmid

   #10978

   Depositor

   Thomas Tuschl

   Article

   Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

   Insert

   TNRC6B (TNRC6B Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmGFP10C-­Tau

    Plasmid

    #71433

    Purpose

    Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71434

    Depositor

    Henri Huttunen

    Article

    Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

    Insert

    Tau (0N4R) (MAPT Human)
    

    Use

    Tags

    GFP (1-212)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

11. GFP-p53

    Plasmid

    #12091

    Depositor

    Tyler Jacks

    Article

    Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    72R variant of p53

    Promoter

    Available since

    July 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMA7CR_2.0

    Plasmid

    #73950

    Purpose

    pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

    Depositor

    Alex Nielsen

    Article

    Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

    Inserts

    cas9
    lambda beta
    dam
    recX
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pAra and pTet

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAS_4xMx PylT_GFP 150TAG

    Plasmid

    #140016

    Purpose

    Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG, K46E (Please see depositor comments below)

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-LIC

    Plasmid

    #26094

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pQdC12a

    Plasmid

    #191356

    Purpose

    Clostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expression

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Inserts

    PGusA2-TetO2/1-dCas12a (LbCpf1)
    miniPthl-tetR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PGus2A-TetO2/1 and miniPthl

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NMD2

    Plasmid

    #216220

    Purpose

    Nonsense mediated decay GFP P2A RFP reporter with two b-globin introns

    Depositor

    Rebecca Voorhees

    Article

    Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

    Insert

    GFP P2A RFP_b-globin intron x2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFU-GW-sMYH6-mNeon-TNNT2-mScarlet

    Plasmid

    #170712

    Purpose

    dual fluorescent reporter for MYH6 (short) and TNNT2 promoters

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Inserts

    mNeonGreen
    mScarlet
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TNNT2 and aMHC

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKH115_GALNT2-keima

    Plasmid

    #214969

    Purpose

    Golgiphagy flux reporter GALNT2-keima

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    GALNT2-keima (GALNT2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDZ416 (24PP7SL loxP-Kan-loxP)

    Plasmid

    #45163

    Depositor

    Robert Singer, Daniel Zenklusen

    Article

    Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

    Insert

    24xPP7 Stem Loop Cassette
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 2C::tdTomato Reporter

    Plasmid

    #40281

    Depositor

    Samuel Pfaff

    Article

    Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

    Insert

    2C Promoter (2-cell stage promoter)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge