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ancBE4-SpRY

Plasmid

#226855

Purpose

Expresses ancBE4max-SpRY base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

ancBE4max-SpRY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGH045_EGFP

Plasmid

#85411

Purpose

lentiviral expression vector for GFP

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFUS_A2A

Plasmid

#43849

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pphiLOV3-N1

Plasmid

#178973

Purpose

Expresses phiLOV3 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

phiLOV3

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->GssmCherry-PNE

Plasmid

#162157

Purpose

Lentiviral vector for constitutive expression of mCherry and PNE anchor in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMALB

Plasmid

#161784

Purpose

Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.

Depositor

John Dick, Peter van Galen

Article

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

Bidirectional minhCMV and hPGK

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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PB_TRE_Z17-PDGFR_EF1a_mCherry

Plasmid

#186303

Purpose

Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

Z17-PDGFR
mCherry

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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sZF4

Plasmid

#43835

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF4

Tags

HA Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
PEmax_2star_IVT

Plasmid

#227674

Purpose

plasmid for PEmax** mRNA IVT

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

PEmax_2_star

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDAS12222_U6-pegRNA-BFP

Plasmid

#177181

Purpose

pegRNA cloning plasmid with BFP marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMP71-CD40L

Plasmid

#174595

Purpose

Stably expresses the extracellular and transmembrane domain of murine CD40L

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

CD40L

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b-pA-Mnase

Plasmid

#124883

Purpose

To transform BL21-Gold(DE3) and induced by IPTG for His-proteinA-MNase fusion protein.

Depositor

Keji Zhao

Article

Ku et al Genome Res. 2021 Apr 14. pii: gr.260893.

Insert

protein A-MNase

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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MTK4_001

Plasmid

#123810

Purpose

Encodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Bgh pA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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HA-CHMP2A

Plasmid

#131453

Purpose

Expresses HA-tagged human CHMP2A in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Insert

human CHMP2A

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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4xmEGFP

Plasmid

#127801

Purpose

Homo-tetramer mEGFP-mEGFP-mEGFP-mEGFP expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mEGFP-mEGFP-mEGFP-mEGFP

Expression

Mammalian

Available Since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBW121-SS-315

Plasmid

#68786

Purpose

8x Target 1 0gap with SV40 Ori

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Insert

8x 0gap Target 1 with SV40 ori

Expression

Mammalian

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-flag-GFP-Intb2

Plasmid

#205194

Purpose

This vector encodes of a synCAM (intb2) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM Intb2 and GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSG28-J23151-AraC

Plasmid

#102453

Purpose

Expresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

AraC (araC )

Expression

Bacterial

Promoter

J23151

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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0_T7-mScarlet3

Plasmid

#225927

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

Depositor

Michalis Averof

Article

Karapidaki et al Development. 2026 Apr 15;153(8):dev20541

Insert

mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSB223

Plasmid

#164127

Purpose

New measurement standard

Depositor

Christopher Voigt

Article

Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

Insert

YFP-PP7

Expression

Bacterial

Promoter

J23101

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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