170,291 results

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EHD2-T72A-mEGFP

Plasmid

#45934

Purpose

dominant-negative EHD2 mutant

Depositor

Ari Helenius

Article

Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

Insert

Eps-15 homology domain-containing protein2 (EHD2 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

T 72 changed to A (T72A)

Promoter

CMV

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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CIRC-Scarless-Ana-CVB3 IRES split 403-mCherry

Plasmid

#246753

Purpose

Generate scarless circRNA with CVB3 IRES

Depositor

Wensheng Wei

Article

Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

Insert

CIRC-Scarless-Ana-CVB3 IRES split 403-mCherry

Use

Synthetic Biology

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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NM1-5S-tRNA-SgH

Plasmid

#128178

Purpose

Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

gRNA cloning cassette

Use

CRISPR

Expression

Yeast

Promoter

5S rRNA-tRNA(Arg)

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-PD-L1

Plasmid

#121492

Purpose

Expression of human PD-L1 WT in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1 (CD274 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A3 WT

Plasmid

#195849

Purpose

Expresses wild-type Homo sapiens ATP13A3

Depositor

Veerle Baekelandt

Article

Houdou et al Biomolecules. 2023 Feb 9;13(2):337. doi:

Insert

ATPase 13A3 (ATP13A3 Human)

Use

Lentiviral

Mutation

D498N, L956P, M850I, V855M, R858H, L675V

Promoter

CMV

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-ATG14

Plasmid

#203565

Purpose

Mammalian expression of ATG14

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG14 (ATG14 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG14

Plasmid

#203566

Purpose

Mammalian expression of GST tagged ATG14

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG14 (ATG14 Human)

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2023

Availability

Academic Institutions and Nonprofits only

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YSBE 517 : pNEO_6xHA

Plasmid

#92083

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, Neo selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

6xHA

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTH100

Plasmid

#84458

Purpose

pJB38-NWMN29-30 + SarA_P1-sGFP-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-sGFP from pCM29

Tags

6xHis (on sfGFP)

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21ΔRING-Box

Plasmid

#105520

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-KRAS-Q61R

Plasmid

#116425

Purpose

Lentiviral expression of KRAS Q61R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

Q61R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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SRPK1-NanoLuc Fusion Vector

Plasmid

#237178

Purpose

Express SRPK1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

SRPK1 (SRPK1 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHelper-CBE-sgRNA

Plasmid

#221137

Purpose

Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

sgRNA-scaffold with Cas9 handle

Use

CRISPR

Expression

Bacterial

Promoter

J23119(SpeI)

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSerOTS-C1* (V70)

Plasmid

#188537

Purpose

Phosphoserine incorporation into recombinant proteins

Depositor

Jesse Rinehart

Article

Mohler et al Mol Syst Biol. 2023 Aug 8;19(8):e10591.

Inserts

pSerRS
Ef-pSer
tRNApSer

Expression

Bacterial

Mutation

mutations resulting from laboratory evolution

Promoter

glnS*

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Dox-Akt

Plasmid

#207328

Purpose

PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)

Depositor

Jared Toettcher

Article

Underhill et al Development. 2023 Aug 15;150(16):dev2016

Insert

rac protein kinase-alpha (AKT1 Human)

Tags

myristoylation tag - BFP

Expression

Bacterial

Mutation

range: 129 - 480

Promoter

Tet Response Element

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMaster12

Plasmid

#58527

Purpose

Reprogram somatic cells to iPS cells

Depositor

Mario Capecchi

Article

Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

Inserts

neo
tk
oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 Human)
Myc (MYC Human)
EBNA-1
Amp
Lin28 (LIN28A Human)
NANOG (NANOG Human)
IRES
NR5A2 (NR5A2 Human)
miR302/367 (MIR367, MIR302A Human)

Expression

Mammalian

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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hnRNPA1-FLAG-IRES-eGFP

Plasmid

#234619

Purpose

Mammalian expression of full-length hnRNPA1 with C-terminal FLAG tag co-expressing with eGFP via IRES sequence

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C-terminal FLAG tag, co-expressing with eGFP via …

Promoter

CMV

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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Membrane mCherry

Plasmid

#210470

Purpose

Plasma membrane localizing mCherry using Human LCK proto-oncogene sequence

Depositor

Amro Hamdoun

Insert

LCK membrane mCherry (LCK Human, A.victoria, Synthetic)

Use

Synthetic Biology; Sea urchin, mammal, zebrafish

Tags

mCherry

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL2 G116A

Plasmid

#123104

Purpose

Expresses 3xFLAG-GABARAPL2 G116A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pOpen-Tthlig

Plasmid

#165569

Purpose

Taq DNA Ligase

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Tth DNA Ligase

Use

Synthetic Biology

Available Since

Oct. 25, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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