19,824 results

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pEGFP-C1-mApg5

Plasmid

#22958

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK2

Plasmid

#101296

Purpose

Mammalian expression of Rock2-GFP

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock2 (Rock2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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Dyn2-pmCherryN1

Plasmid

#27689

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Dynamin 2 (DNM2 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Neo DEST (685-3)

Plasmid

#17292

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pU6a:sgRNA#2

Plasmid

#64246

Purpose

expresses sgRNA under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-RNF2_+5GtoT

Plasmid

#135957

Purpose

S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_5GtoT pegRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1-GalT

Plasmid

#87327

Purpose

To express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

Depositor

Lei Lu

Article

Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

Insert

1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Rheb1 shRNA #1

Plasmid

#26625

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Rheb1 shRNA (RHEB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-DIO-eGFP-2A-TetanusToxin

Plasmid

#166603

Purpose

Encodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

EGFP-2A-Tetanus Toxin

Use

AAV

Promoter

tetracycline response element

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET N6his GFP1-9

Plasmid

#182240

Purpose

Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

Depositor

Stéphanie Cabantous

Article

Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

Insert

GFP1-9

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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hCathepsin S

Plasmid

#11251

Purpose

Mammalian expression of human Cathepsin S

Depositor

Hyeryun Choe

Article

Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

Insert

cathepsin S (CTSS Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pCL-CTIG

Plasmid

#14901

Purpose

3rd generation lentiviral vector expressing tTA-VP16 and EGFP.

Depositor

Inder Verma

Article

Kafri et al Mol Ther. 2000 Jun . 1(6):516-21.

Insert

CMV-tTA-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-HTG

Plasmid

#27437

Depositor

Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

histone-GFP-2A-TVA receptor-2A-rabies glycoprotein

Use

AAV; Adeno-associated viral

Mutation

N/A

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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DsRed-rab7 DN

Plasmid

#12662

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

DsRed and HA

Expression

Mammalian

Mutation

T22N

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pORANGE LOX Cloning template vector

Plasmid

#139651

Purpose

Cloning template for ORANGE-CAKE conditional knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt5A

Plasmid

#35911

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-K9 histone methylation reporter

Plasmid

#22866

Depositor

Alice Ting

Article

Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

Insert

K9 HMR

Tags

CFP and YFP

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.3-NL63-D14

Plasmid

#172666

Purpose

encodes NL63 spike protein, for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

HCNV63gp2 (HCNV63gp2 human coronavirus NL63)

Use

Lentiviral and Retroviral

Tags

NA

Expression

Mammalian

Mutation

c-terminal deletion of 14aa

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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UbC-TRE-mSOD2-tTS-IRES-EGFP

Plasmid

#12390

Depositor

Zuoshang Xu

Article

Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

Insert

mSOD2 shRNA (Sod2 Mouse)

Use

RNAi

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pUHD Cdk1 WT HA

Plasmid

#27652

Depositor

Greg Enders

Insert

Cdk1 (CDK1 Human)

Tags

HA (YDVPDYA)

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only