We narrowed to 15,686 results for: genome

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pICSL11060

Plasmid

#68264

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pX330.sgNf1.4

Plasmid

#92028

Purpose

pX330 sgNf1.4 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.4

Use

Mouse Targeting

Expression

Mammalian

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only
pYTSK01K_0G7

Plasmid

#177296

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

Depositor

Karl-Erich Jaeger

Article

Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

Insert

PaacC1-aacC1

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pJEP304-pAAV-EFS-dSaCas9-VP64-pA

Plasmid

#113679

Purpose

A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV, CRISPR, and Synthetic Biology

Tags

NLS and VP64

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBW_0065

Plasmid

#102817

Purpose

pVec8 containing Sr22 gene

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

wheat stem rust resistance gene Sr22 (NEWENTRY Synthetic)

Expression

Plant

Promoter

Native

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBW_0059

Plasmid

#102819

Purpose

pAGM4723 containing Sr35 gene

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

wheat stem rust resistance gene Sr35 (NEWENTRY Synthetic)

Use

Synthetic Biology

Promoter

Native

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-HA-CCPG1

Plasmid

#139867

Purpose

Lentiviral expression of HA-tagged human CCPG1

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

CCPG1 (CCPG1 Human)

Use

Lentiviral

Tags

HA

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLV302

Plasmid

#119943

Purpose

Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

Depositor

Gerald Schwank

Article

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ10713

Plasmid

#183958

Purpose

U6-crSox2-CAG-hyperdCas12a-miniVPR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Inserts

HyperdCas12a
crRNA targeting Sox2

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CAG and U6

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Clta KI

Plasmid

#131483

Purpose

Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Clta Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pRDB_181

Plasmid

#216102

Purpose

CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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gh76

Plasmid

#106747

Purpose

expression of gRNA targeting MGAT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

MGAT1 (MGAT1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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A3G-BE5.14

Plasmid

#163638

Purpose

C to T DNA base editing

Depositor

Xue Gao

Article

Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

Insert

A3G-BE5.14

Tags

BPSV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCSF107mT-GATEWAY-3'-LAP tag

Plasmid

#67618

Purpose

A pCS2 based vector to generate a 3'-LAP (eGFP-TEV-S) tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-termnal LAP (eGFP-TEV-S)

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
A3G-BE5.13

Plasmid

#163637

Purpose

C to T DNA base editing

Depositor

Xue Gao

Article

Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

Insert

A3G-BE5.13

Tags

BPSV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HP1α-miRFP670-Cry2WT

Plasmid

#122442

Purpose

Expresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

HP1 alpha (CBX5 Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Promoter

SFFV

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9_U6_sgRNA_Mettl3C

Plasmid

#165420

Purpose

sgRNA construct for targeting Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1

Plasmid

#176250

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozym

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR and Synthetic Biology; Expression in micro…

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pORANGE CACNG8-GFP KI #2

Plasmid

#131474

Purpose

Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: A408)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng8 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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AA331

Plasmid

#215954

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v2 [Sa]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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