We narrowed to 22,070 results for: his

1. A2M-bio-His

   Plasmid

   #53423

   Purpose

   Expresses full-length Alpha-2-macroglobulin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   A2M (A2M Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAS9-mCherry-HIST1H4C

   Plasmid

   #66944

   Purpose

   CRISPaint target selector HIST1H4C

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA HIST1H4C
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pprm1-luciferase; his-3

   Plasmid

   #137728

   Purpose

   Communication-activated luciferase in Neurospora crassa

   Depositor

   Louise Glass

   Article

   Fischer et al Genetics. 2019 Apr;211(4):1255-1267. doi

   Inserts

   Luciferase
   Pprm-1 Promoter
   

   Use

   Luciferase; Neurospora crassa expression

   Tags

   Expression

   Mutation

   Codon-optimized for N. crassa

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA CRU HISMX

   Plasmid

   #131164

   Purpose

   PCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutant

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Use

   Tags

   CRU

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a(His10-PD-1cyto)

   Plasmid

   #177896

   Purpose

   E coli expression of His10 tag fused to human PD-1 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   PD-1 (aa 194-288 only) (PDCD1 Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29b-ClbPpep-CHis

   Plasmid

   #48243

   Purpose

   First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbP-pep
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   First 375 amino acids of the peptidase, which con…

   Promoter

   T7

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNIC-HIS6-SUMO1-GG

   Plasmid

   #215799

   Purpose

   Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. UbG76V-eGFP-V5His_15aa

   Plasmid

   #23969

   Depositor

   Nico Dantuma

   Article

   Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

   Insert

   Ub-G76V-eGFP-V5HIS_15aa (UBB Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   G76V mutation in Ubiquitin. Ub fused to N-termin…

   Promoter

   Available since

   April 16, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pDEST40-ARF6-V5-His6

   Plasmid

   #67436

   Purpose

   Mammalian expression of hArf6 with C-term V5 and His6 tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. ET8-GPIba-6His

    Plasmid

    #102878

    Purpose

    Wild type human platelet membrane protein GPIb alpha (1-290aa) with 6Histag at the C-terminus

    Depositor

    Timothy Springer

    Article

    Fu et al Nat Commun. 2017 Aug 23;8(1):324. doi: 1

    Insert

    Human platelet membrane protein GPIb alpha 1-290aa (GP1BA Human)
    

    Use

    Tags

    6xHis tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMBP-HIS2-Vps20_deltaC

    Plasmid

    #21491

    Depositor

    James Hurley

    Article

    Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

    Insert

    Vps20 Delta_C (VPS20 Budding Yeast)
    

    Use

    Tags

    6x His and MBP

    Expression

    Bacterial

    Mutation

    bp 511-663 deleted

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pET28_6xHis-PARP1-Y986H

    Plasmid

    #174801

    Purpose

    Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986H (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1-Y986S

    Plasmid

    #174802

    Purpose

    Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986S (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28_6xHis-PARP1-G972R

    Plasmid

    #174800

    Purpose

    Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G972R (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G972R, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. 6xHis-TEV-UBC-9

    Plasmid

    #87055

    Purpose

    Expresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORF

    Depositor

    Ronald Hay

    Article

    Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

    Insert

    UBC-9 (ubc-9 Nematode)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-sACE2v3-8his

    Plasmid

    #149271

    Purpose

    Mammalian expression plasmid for his-tagged sACE2 high affinity variant 3

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGPD-luxAB(HIS)

    Plasmid

    #1106

    Depositor

    Susan Lindquist

    Article

    Parsell et al Nature 1994 Dec 1;372(6505):475-8.

    Insert

    bacterial luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    n/a

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pFA6a-PA-GFP-His3MX6

    Plasmid

    #18822

    Depositor

    Wei-Lih Lee

    Article

    Vorvis et al Yeast. 2008 Sep;25(9):651-9.

    Insert

    Photoactivatable Green Fluorescent Protein
    

    Use

    Yeast tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDB.His.MBP.3C NLRP1 CARD WT

    Plasmid

    #164008

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac(His10-Shp2)

    Plasmid

    #177899

    Purpose

    Baculo expression of His10 tag fused to human Shp2

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    Shp2 (PTPN11 Human)
    

    Use

    Tags

    10xHis-TEV-SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. F2R-bio-His

    Plasmid

    #51863

    Purpose

    Expresses full-length Proteinase-activated receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    F2R (F2R Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-sACE2v2-8his

    Plasmid

    #149270

    Purpose

    Mammalian expression plasmid for his-tagged sACE2 high affinity variant 2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PEAR1-bio-His

    Plasmid

    #51860

    Purpose

    Expresses full-length Platelet endothelial aggregation receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PEAR1 (PEAR1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBad-HisB-GGvT

    Plasmid

    #87362

    Purpose

    Expresses GGvT, a tandem homodimer green fluorescent protein

    Depositor

    Robert Campbell

    Article

    Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

    Insert

    GGvT
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-Hisx6-Sirt6

    Plasmid

    #20277

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pRS424-TUB2-Cterminal6xHis

    Plasmid

    #60386

    Purpose

    Expression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    6xHIS

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pGEX-DAXX-ΔD/E-His

    Plasmid

    #179618

    Purpose

    Express GST-DAXX-ΔDE-His in bacteria

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 424-495

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LAMP1-bio-His

    Plasmid

    #51809

    Purpose

    Expresses full-length Lysosome-associated membrane glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NELFE-mOrange pHis-//

    Plasmid

    #53303

    Purpose

    Expresses His-tagged NELF-E-mOrange fusion protein.

    Depositor

    John Lis

    Article

    Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

    Insert

    NELF-E protein (Nelf-E Fly)
    

    Use

    Tags

    His and mOrange

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-6His-MyoX

    Plasmid

    #47607

    Depositor

    Emanuel Strehler

    Article

    Bennett et al Biochem Biophys Res Commun. 2008 May 2;3

    Insert

    MyoX (MYO10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q680R

    Promoter

    CMV

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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