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Plasmid#234411PurposeExpresses the chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut and a membrane-localized EGFP in mammalian cellsDepositorInsertGPCR activation based chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX2-tTA2-WPRE-bGHpA
Plasmid#65458PurposeCan be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent mannerDepositorInserttTA2
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA
Plasmid#65455PurposeCan be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinasesDepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pvalb-2A-Cre targeting vector
Plasmid#61570PurposeTarget the Cre recombinase gene to the stop codon of the mouse Pvalb geneDepositorInsertPvalb exon 4 - 2A - Cre
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pvalb-2A-Flpo Flp-in replacement vector
Plasmid#61572PurposeRecombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codonDepositorInsertPvalb-2A-Flpo
UseRecombinase-mediated cassette exchange using flp …TagsExpressionMutationPromoterAvailable sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Snap25-LSL-2A-GFP targeting vector
Plasmid#61575PurposeTarget a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 geneDepositorInsertSnap25-LSL-2A-GFP
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA
Plasmid#65453PurposeCan be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinasesDepositorHas ServiceAAV1InsertEGFP
UseAAVTagsExpressionMutationPromotertetOAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FlpE-bGHpA
Plasmid#50364PurposeCan be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoterDepositorInsertFlpE recombinase gene
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-CBA-dt/PGK-CFTR-⌀
Plasmid#163923PurposeExpresses human CFTR.DepositorInsertCFTR (CFTR Human)
UseLentiviralTagsExpressionMammalianMutationWTPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hPEN2 D90A
Plasmid#183641PurposeLentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the C-terminus (lysosome lumen).DepositorInsertPEN2 (PSENEN Human)
UseLentiviralTagsHAExpressionMutationresidues 90 of PEN2 mutated to alaninePromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hPEN2 2A
Plasmid#183640PurposeLentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).DepositorInsertPEN2 (PSENEN Human)
UseLentiviralTagsHAExpressionMutationresidues 35 and 40 of PEN2 mutated to alaninePromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iCreV
Plasmid#140135PurposeCan be used to generate AAV virus that will express light-inducible site-specific iCreV recombinaseDepositorHas ServiceAAV PHP.eB and AAV1InsertiCreV
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rasgrf2-2A-dCre targeting vector
Plasmid#61573PurposeTarget a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 geneDepositorInsertRasgrf2-2A-dCre (Rasgrf2 P1 bacteriophage, Mouse)
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMarch 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hGOLGA2-C-PEN2
Plasmid#183649PurposeLentiviral expression of a golgi-residential PEN2.DepositorUseLentiviralTagsHAExpressionMutationWTPromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FlpO-bGHpA
Plasmid#51669PurposeCan be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter.DepositorHas ServiceAAV RetrogradeInsertFlpO recombinase gene
UseAAVTagsExpressionMammalianMutationPromoterhSyn1Available sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
Plasmid#65454PurposeCan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinasesDepositorInsertChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMutationH134R variantPromoterhSyn1Available sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ai62(TITL-tdT) Flp-in replacement vector
Plasmid#61576PurposeRecombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locusDepositorInsertchromatin insulator flanked TRE-LSL-tdTomato
UseRecombinase-mediated cassette exchange using flp …TagsExpressionMutationPromoterAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hLAMP2-C-PEN2
Plasmid#183646PurposeLentiviral expression of a lysosome-residential PEN2.DepositorUseLentiviralTagsHAExpressionMutationWTPromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hTOMM20-C-PEN2
Plasmid#183647PurposeLentiviral expression of a mitochondrion-residential PEN2.DepositorUseLentiviralTagsExpressionMutationWTPromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL1 sgRNA(MS2)-MS2-AIDmono
Plasmid#112127Purposeempty sgRNA cloning vector with MS2-AIDmonoDepositorInsertAID mono mutant (AICDA Human)
UseCRISPRTagsExpressionMammalianMutationN- mutated and C- terminal truncated, H130A/R131E…PromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL3 sgRNA(MS2)-MS2-AID3f
Plasmid#112129Purposeempty sgRNA cloning vector with MS2-AID3fDepositorInsertAIDmono 3f mutant (AICDA Human)
UseCRISPRTagsExpressionMammalianMutationN- mutated and C- terminal truncated, F115Y/C116F…PromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL2 sgRNA(MS2)-MS2-AID3c
Plasmid#112128Purposeempty sgRNA cloning vector with MS2-AID3cDepositorInsertAID mono 3c mutant (AICDA Human)
UseCRISPRTagsExpressionMammalianMutationN- mutated and C- terminal truncated, F115Y/C116F…PromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
Plasmid#65429PurposeCan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinasesDepositorInsertChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMutationH134R variantPromoterhSyn1Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pvalb-2A-Dre Flp-in replacement vector
Plasmid#61571PurposeRecombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codonDepositorInsertPvalb-2A-Dre (Pvalb D6 Bacteriophage, Mouse)
UseRecombinase-mediated cassette exchange using flp …TagsExpressionMutationPromoterAvailable sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-hSOX2-C17
Plasmid#206385PurposeExpresses human SOX2-C17 in mammalian cells, for lentivirus generation.DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hAP1 1-419
Plasmid#183644PurposeTo (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expressionDepositorInsertATP6AP1 (ATP6AP1 Human)
UseTagsmycExpressionMammalianMutationtruncated ATP6AP1 mutant; contains only amino aci…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hAP1 Δ420-440
Plasmid#183642PurposeTo (stably) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; lentiviral expressionDepositorInsertATP6AP1 (ATP6AP1 Human)
UseLentiviralTagsHAExpressionMutationtruncated ATP6AP1 mutant lacking the transmembran…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hSEC61B-C-PEN2
Plasmid#183648PurposeLentiviral expression of a ER-residential PEN2.DepositorUseLentiviralTagsHAExpressionMutationWTPromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYY166 pcDNA3.4-His-MBP-hAID
Plasmid#231942PurposeExpression of His-MBP-AID fusion protein in mammalian cellsDepositorInsertAID (AICDA Human)
UseTagsHis-MBPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
MOS
Plasmid#64120PurposeA modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genesDepositorUseTagsHA and MycExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
MMK
Plasmid#64121Purposea modified episomal (EBNA1/OriP) vector expressing human Myc and KLF4 genesDepositorUseTagsExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pattB-LSL-AAVR-F2A-spCas9
Plasmid#202459PurposeThe plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.DepositorInsertAAVR (AU040320 Mouse)
UseCRISPR, Cre/Lox, and Mouse TargetingTagsmCherry fusionExpressionMammalianMutationPromoterCAGAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hAP1 420-440
Plasmid#183643PurposeTo (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expressionDepositorInsertATP6AP1 (ATP6AP1 Human)
UseTagsmycExpressionMammalianMutationtruncated ATP6AP1 mutant; contains only the trans…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hAP1-C-LAMP2 TM
Plasmid#183645PurposeLentiviral expression of a mutant of ATP6AP1 with its PEN2-interacting domain replaced with the transmembrane (TM) domain of LAMP2.DepositorUseLentiviralTagsHAExpressionMutationATP6AP1 transmembrane domain replaced by the tran…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-Flag-HA
Plasmid#63681Purposeadenoviral shuttle vector containing N-terminal Flag tagDepositorTypeEmpty backboneUseAdenoviralTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dendra2-NS3
Plasmid#238004PurposeExpresses NS3 in mammalian/mouse cell lineDepositorInsertNS3
UseTagsDendra2ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pANY4
Plasmid#164897PurposeTwo valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.DepositorInsertccdB cassette
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-Beta
Plasmid#177962PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G)DepositorInsertSARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G) (S )
UseTagsExpressionMammalianMutationK417N, E484K, N501Y, D614GPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-Epsilon
Plasmid#177964PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G)DepositorInsertSARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G) (S )
UseTagsExpressionMammalianMutationS13I, W152C, L452R, D614GPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-K417N
Plasmid#177969PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, K417N)DepositorInsertSARS-CoV-2 Spike protein (D614G, K417N) (S )
UseTagsExpressionMammalianMutationD614G, K417NPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only