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Plasmid#176758PurposeAAV-mediated expression of GCaMP8s under the EF1a promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344A-mCerulean
Plasmid#69580PurposeExpresses p53-L344A mutant tagged with mCeruleanDepositorInsertp53-L344A (TP53 Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationp53 L344A mutation that prevents tetramerization …PromoterEF1alphaAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
p156RRL-EF1a-GFP-U3H1SatA
Plasmid#41795DepositorInsertsatellite repeat sequence
UseTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOTTC414 - pAAV EF1a hPREP
Plasmid#59967PurposeAn AAV packaging vector that expresses hPREP under control of the EF1a promoter.DepositorInsertProlyl oligopeptidase (PREP Human)
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1a-MegaGate-DD-Blast
Plasmid#175271PurposeMegaGate destination with C-terminal DD tag and blasticidin resistanceDepositorTypeEmpty backboneUseTagsDestabilization DomainExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMV-SB100X[EF1α-copGFP]
Plasmid#218323PurposeDonor plasmid of SB100X Transposon with copGFP mammalian expression cassetteDepositorInsertcopGFP mammalian expression cassette
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-DHB-mTurq2
Plasmid#212667PurposeExpress tagged DHBDepositorInsertDHB (HELB Human)
UseLentiviralTagsmTurq2ExpressionMutationPromoterEF-1aAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC293 - pAAV EF1a V5-synuclein (WT)
Plasmid#60057PurposeAn AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.DepositorInsertalpha-synuclein (SNCA Human, human)
UseAAVTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO_EGFP-RandE3
Plasmid#79872PurposeFor generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.DepositorInsertGFP-RandE3
UseAAV, Cre/Lox, and Synthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Fon-tdTomato
Plasmid#72261PurposeExpress tdTomato when FLP is expressed by another vectorDepositorInserttdTomato
UseLentiviralTagsExpressionMammalianMutationT230I in tdTomato (please see depositor's co…Promoterelongation factor-1Available sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRE-STOP EF1a-rtTA3
Plasmid#195882Purposecontrol construct of TRE-VEGFA EF1a-rtTA3DepositorInsertVEGFA (Vegfa Synthetic, Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG
Plasmid#174891Purposeamber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassetteDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAG in sfGFPPromoterEF1Available sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1_-DIO-GRAB_NPY1.0
Plasmid#208677PurposeExpresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in a cre-dependent mannerDepositorInsertGPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
UseAAVTagsExpressionMutationPromoterEF1_Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN5(TA)-ires-puro
Plasmid#167823Purposecontrol sensor for EKAREN5DepositorInsertEKAREN5(TA)
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ef1a-DIO-eGCaMP(2+)
Plasmid#201150PurposeAAV virus production for Cre recombinase dependent expression of eGCaMP(2+)DepositorInserteGCaMP_2+
UseAAV, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV/EF1a-DIO-SCLM
Plasmid#216759PurposeAAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoterDepositorInsertSuperClomeleon
UseAAVTagsExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterEF1a (human elongation factor 1 alpha)Available sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode
Plasmid#170237PurposeBarcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertKRAS (KRAS Human)
UseLentiviralTagsExpressionMutationG12VPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-Cre-PGK-Puro
Plasmid#207742PurposeCo-expression of the Cre recombinase and a puro selectable markerDepositorInsertCre-PGK-Puro
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-EF1a-jGCaMP8m-WPRE
Plasmid#176757PurposeAAV-mediated expression of GCaMP8m under the EF1a promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only