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pHis6-hDF-deltaTM-pCMV-9

Plasmid

#72587

Purpose

human dysferlin no transmembrane domain tagged with his tag

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Tags

His

Expression

Mammalian

Mutation

transmembrane domain deleted

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA_SZ1-RGG-GFP-RGG

Plasmid

#124932

Purpose

Expression of SZ1-RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

GFP

Expression

Mammalian

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLC-HA active Nedd8-P2A-Hygro

Plasmid

#124307

Purpose

Lentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

Nedd8 (NEDD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Five C-terminal amino acids missing compared to w…

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEYFP-hDF-C2A

Plasmid

#72582

Purpose

human dysferlin C2A domain tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin C2A domain (DYSF Human)

Tags

EYFP

Expression

Mammalian

Mutation

C2A domain

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-RFP-RGG

Plasmid

#124934

Purpose

Expression of RGG-RFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-RIAM(1-666)

Plasmid

#80028

Purpose

Mammalian expression of GFP-RIAM(1-666)

Depositor

Chinten James Lim

Article

Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

Insert

RIAM (APBB1IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-666

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
mCherry-Climp63(1–192)

Plasmid

#186955

Purpose

Expresses mCherry-Climp63 with partial luminal domain

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Ckap4 (Ckap4 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only remain partial luminal domain

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-puro HA-Atg14*

Plasmid

#38265

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed A618T, A621T, C624A and T627C for siRNA r…

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CHRFAM7A

Plasmid

#62284

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A)

Depositor

Sherry Leonard, Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Expression

Mammalian

Mutation

partial duplication of CHRNA7

Promoter

CMV

Available Since

March 3, 2015

Availability

Industry, Academic Institutions, and Nonprofits
CSII-IDR2-owlTRIMCyp-FOS

Plasmid

#79067

Purpose

Express owl monkey TRIMCyp in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5Cyp (owl monkey)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+36)-DL4

Plasmid

#199168

Purpose

used to express a supercharged GFP variant that forms inclusion bodies in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+36)-DL4

Tags

MGHHHHHHGGA

Mutation

E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

Promoter

T7

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(chimpanzee)

Plasmid

#79032

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(chimpanzee)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-800)-Strep

Plasmid

#141344

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

Insert

GB1-His6-TEV-ALIX(703-800)-Strep

Tags

GB1-His6-TEV and Strep-tag (WSHPQFEK)

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMtb CblA

Plasmid

#138298

Purpose

Expression of Mtb CblA

Depositor

Ruma Banerjee

Article

Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

Insert

Mtb CblA

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET11a-CA(A14C, E45C, A92E)

Plasmid

#79046

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

CA (A14C, E45C, A92E)

Expression

Bacterial

Mutation

A14C, E45C, A92E mutations in HIV-1 CA protein

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pKM294

Plasmid

#134179

Purpose

Mammalian expression of CENP-C and KNL2

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

mcherry-CENP-C 510-end-KNL2 (CENPC Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RGG_withTEVsite

Plasmid

#124940

Purpose

Expression of RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Mutation

with TEV site between RGG domains

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H

Plasmid

#146503

Purpose

Insect Expression of DmDCP1_327-372

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_327-372 (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b-ITPA

Plasmid

#60824

Purpose

to purify human ITPA from bacteria

Depositor

Youri Pavlov

Article

Porta et al Acta Crystallogr Sect F Struct Biol Crys

Insert

ITPA (ITPA Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RFP-RGG

Plasmid

#124944

Purpose

Exoression of RGG-RFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His and mCherry

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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