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pUC57-Curlcake3

Plasmid

#139342

Purpose

Expresses Curlcake3 (CC3) in E.coli

Depositor

Eva Maria Novoa

Publication

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake3 with T7 promoter

Expression

Bacterial

Promoter

T7

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Ef1a-MCP-huADARE488QT490A

Plasmid

#159922

Purpose

Fusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5

Depositor

Fei Chen

Publication

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

Human ADAR2 E488QT490A (ADARB1 Human)

Use

AAV

Promoter

EF1a

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

Plasmid

#159899

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | tagRFP | cbr-tbb-2 UTR

Expression

Worm

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFUSE-rIgG-Fc2-ICAM1

Plasmid

#156462

Purpose

Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

Depositor

Dirk Mielenz

Publication

Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

Insert

Intercellular Adhesion Molecule 1 (Icam1 Mouse)

Tags

ICAM1-rFc

Expression

Bacterial and Mammalian

Mutation

endogenous signal peptide replaced with IL2 signa…

Promoter

hEF1-HTLC

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-gRNA

Plasmid

#158973

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

Depositor

Charles P. Lai

Publication

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA3

Expression

Mammalian

Promoter

U6

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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Tol2-CAG::Palmbow

Plasmid

#158993

Purpose

Multicolor membrane-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing palmitoylated mCherry, mEYFP or mCerulean upon Cre recombination)

Depositor

Jean Livet

Publication

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Palmbow

Tags

The default expressed EBFP2 is fused to H2B; oth…

Expression

Mammalian

Promoter

CAG

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXO3

Plasmid

#153142

Purpose

Expression of Flag tagged human FOXO3 in mammalian cells

Depositor

Stefan Koch

Publication

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXO3 (FOXO3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Flag-FOXG1

Plasmid

#153124

Purpose

Expression of Flag tagged human FOXG1 in mammalian cells

Depositor

Stefan Koch

Publication

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXG1 (FOXG1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

Plasmid

#155306

Purpose

Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

Depositor

Stanley Qi

Publication

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Rfx Cas13d CRISPR RNA BFP

Expression

Mammalian

Promoter

hU6, EF1a

Available since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAID-EF1a linearizing in pX330

Plasmid

#140610

Purpose

Crispr/Cas9 plasmid for linearization of pAID-EF1a

Depositor

Tatsuo Fukagawa

Publication

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA for pAID-EF1a plasmid digestion

Use

CRISPR

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLVX Nup62Fv2GFP

Plasmid

#139683

Purpose

Nup62 dimerization construct fused to 2 copies of GFP under an CMV promoter

Depositor

Edward Campbell

Publication

Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

Insert

Nup62 (NUP62 Human)

Use

Lentiviral

Tags

2 copies of GFP and Dimerization domain FKBP

Expression

Mammalian

Promoter

CMV

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMP71-hPDL-1

Plasmid

#118631

Purpose

human PDL-1 coding sequence subcloned into pMP71 retroviral plasmid

Depositor

Sébastien Walchli

Publication

Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

Insert

hPDL-1 (CD274 Human)

Expression

Mammalian

Promoter

LTR

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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epiCRISPR

Plasmid

#135960

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Publication

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pAAV-Sox2-CreERT2

Plasmid

#135618

Purpose

Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus

Depositor

Lin He

Publication

Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

Insert

P2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

P2A-CreERT2

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRP.ExTri-CMV-5'UTR-ApoB48-Linker-eGFP

Plasmid

#138334

Purpose

Expresses APOB-Linker-eGFP

Depositor

Mahmood Hussain

Publication

Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

5'UTR-ApoB48-Linker-eGFP (APOB Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YEE-BE4max

Plasmid

#138157

Purpose

C-to-T base editor

Depositor

David Liu

Publication

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rAPOBEC1(YEE)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGEX-4T-1-3xFLAG

Plasmid

#129570

Purpose

Empty backbone for FLAG tag

Depositor

Kevin Janes

Publication

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mNeongreen-PXN

Plasmid

#129604

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Paxillin

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-TBK1-C-FLAG-HA

Plasmid

#131792

Purpose

Lentiviral vector expressing WT TBK1 containing STOP codon

Depositor

Alicia Pickrell

Publication

Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

Insert

TBK1 WT (TBK1 Human)

Use

Lentiviral

Tags

STOP codon

Expression

Mammalian

Mutation

WT full length

Promoter

CMV

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC16A3

Plasmid

#131899

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SLC16A3 (SLC16A3 Human)

Expression

Mammalian

Available since

Oct. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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