Skip to main content
Addgene
Showing: 1721 - 1760 of 4361 results
  1. pSR04

    Plasmid
    #69151
    Purpose
    read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV
    Inserts
    mCherry
    TagBFP
    eGFP
    Use
    Cre/Lox; Mossci
    Tags
    Expression
    Worm
    Mutation
    codon-optimzed index 1.0
    Promoter
    eef-1A.1 (eft-3) and rps-27
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. [#GS24 -LV] Syn1-GFP-OMP25

    Plasmid
    #233566
    Purpose
    Lentiviral construct of mitochondrial localized GFP to be expressed in the donor cells under the Synapsin1 promotor
    Depositor
    Insert
    eGFP (eGFP Synthetic, Aequorea victoria)
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid
    #173208
    Purpose
    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.
    Depositor
    Insert
    ATP1A1-T804N mScarlet-I-TOSI donor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK1
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pRRL-VinculinTS-S1033A

    Plasmid
    #211835
    Purpose
    Vinculin tension sensor (VinTS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.
    Depositor
    Insert
    VinculinTS-S1033A (VCL Chicken, Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to alanine (S1033A),…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pRRL-VinculinTS-S1033D

    Plasmid
    #211893
    Purpose
    Vinculin tension sensor (VinTS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.
    Depositor
    Insert
    VinculinTS-S1033D (VCL Chicken, Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to aspartic acid (S1…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pRRL-VinculinCS-S1033A

    Plasmid
    #211894
    Purpose
    Vinculin conformation sensor (VinCS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.
    Depositor
    Insert
    VinculinCS-S1033A (VCL Chicken, Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to alanine (S1033A),…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pRRL-VinculinCS-S1033D

    Plasmid
    #211895
    Purpose
    Vinculin conformation sensor (VinCS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.
    Depositor
    Insert
    VinculinCS-S1033D (VCL Chicken, Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to aspartic acid (S1…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pDD2604

    Plasmid
    #175088
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S644A
    Promoter
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pDD2602

    Plasmid
    #175086
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T424A, T438A
    Promoter
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pDD2603

    Plasmid
    #175087
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T504A, T508A
    Promoter
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. ATP1A1_G3_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid
    #178096
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.
    Depositor
    Insert
    ATP1A1 G3 + MTOR nick exon-44 sgRNAs
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA

    Plasmid
    #178099
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.
    Depositor
    Insert
    ATP1A1 G3 + MTOR nick intron-45 sgRNAs
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 1721 - 1760 of 4361 results