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pRG646_lexO-HO_LexA-TF_LEU2MX

Plasmid

#154815

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pUDP293

Plasmid

#204228

Purpose

Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA

Depositor

Jean-Marc Daran

Article

Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

Inserts

Eubacterium rectale cas12a (Ercas12a)
BsaI_GFP drop-out_BsaI

Use

Synthetic Biology

Tags

NLS

Mutation

codon optimized for S. cerevisiae

Promoter

Saccharomyces cerevisiae PGK1 and Saccharomyces c…

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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psPAX2-D64V-NC-COM

Plasmid

#131226

Purpose

Expressing GAG precursor protein, in which COM is inserted after the second zinc finger domain of nucleocapsid protein (NC)

Depositor

Baisong Lu

Article

Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

Insert

COM

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos

Plasmid

#211840

Purpose

Plant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

HA and mCherry

Expression

Plant

Promoter

UBIQUITIN10

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179012

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQAT43

Plasmid

#223415

Purpose

T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold

Use

CRISPR; T-dna vector

Tags

NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSIBR004

Plasmid

#177667

Purpose

SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

Depositor

John van der Oost

Article

Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

lacUV5

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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hCdh1 (1-125)

Plasmid

#8508

Depositor

Joan Ruderman

Article

Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

Insert

hCdh1 (1-125) (FZR1 Human)

Use

Retroviral

Tags

His

Expression

Mammalian

Mutation

Cdh1 N-terminus. Dominant Negative mutant.

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
CTB

Plasmid

#141166

Purpose

Expresses recombinant Cholera Toxin B Subunit

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

CTB

Expression

Bacterial

Mutation

No secretion signal

Promoter

T7

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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RUBY-ABE#2

Plasmid

#232176

Purpose

T-DNA vector with ecTadA8e-zCas9D10A and corresponding sgRNA to correct RUBYm2 and recover a vivid red color visible to the naked eyes.

Depositor

Yiping Qi

Article

Sexton et al Plant Physiol. 2025 Jul 3;198(3):kiaf265

Insert

2x35s-ecTadA8e-SpCas9-D10A-AtU3-sgRNA-mis4-gRNA scaffold

Use

CRISPR

Tags

3xflag and NLS

Expression

Plant

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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BiP-mCherry

Plasmid

#62233

Purpose

Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mCherry-KDEL

Tags

mCherry

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-TaGu-ENT-3xF-ORF

Plasmid

#238488

Purpose

For production of C-term flag-tagged TaGu EN-tuned ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Mutation

R1109->A

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL-mU6-GFPGO-IRES-mScarletI

Plasmid

#136895

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO2.

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPGO-IRES-mScarletI

Use

Lentiviral

Tags

3xNLS on mScarlet

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA 2 GAPDH

Plasmid

#83809

Purpose

Express Sg-2 sgRNA targeting GAPDH

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

SgRNA

Use

CRISPR

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos

Plasmid

#211843

Purpose

Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

HA and mCherry

Expression

Plant

Mutation

C492A

Promoter

UBIQUITIN10

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT001

Plasmid

#182711

Purpose

aTc inducible dCasRx

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

2x-FLAG

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-AAVS1_sgRNA

Plasmid

#183891

Purpose

pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pUASTattB-GFP-Msp300KASH

Plasmid

#170806

Purpose

Expresses Msp300 KASH domain fused to GFP under Gal4/UAS control for Drosophila transgenesis

Depositor

Vikki Weake

Article

Jauregui-Lozano et al Genetics. 2021 May 22. pii: 6281219. doi

Insert

GFP-Msp300KASH

Tags

GFP

Expression

Insect

Promoter

5xUAS + hsp70 minimal promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pUC-AdEvolve-DEST

Plasmid

#131692

Purpose

A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Type

Empty backbone

Use

Adenoviral

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Rheb

Plasmid

#133767

Purpose

Expresses GFP-tagged Rheb in mammalian cells

Depositor

Shawn Ferguson

Article

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Insert

Rheb (RHEB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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