Addgene

We narrowed to 13,557 results for: TIM

Showing: 2201 - 2250 of 13557 results

Results per page:

Sort results by:

pETM6-P.accABCD

Plasmid

#66866

Purpose

Encodes accA, accB, accC, accD in Pseudo-operon configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

accA
accB
accC
accD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-mYPet

Plasmid

#103988

Purpose

Plasmid encoding mYPet

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mYPet

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, I47L, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIII-Neo

Plasmid

#153422

Purpose

MSCV with IRES-Neomycin resistant gene

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoPA-TagRFP-SpHis5

Plasmid

#44854

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable TagRFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-TagRFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB783

Plasmid

#60162

Purpose

Firefly luciferase (Promega) driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Use

Tags

Expression

Yeast

Mutation

A4V & S504G (no functional effect)

Promoter

RNR1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pExo1

Plasmid

#53357

Purpose

Expresses beta-lactamase for the degradation of beta-lactam antibiotics and tags it with hlyAs (transporter gene) in preparation for secretion from the cell.

Depositor

Lingchong You

Article

Pai et al Proc Natl Acad Sci U S A. 2012 Nov 27;10

Inserts

BlaMs
hlyAs

Use

Tags

Expression

Mutation

Promoter

p(lac/ara-1)

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
CS2 Flag-Smad3 (EPSM)

Plasmid

#14963

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Insert

Smad3 (EPSM) (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

T178V, S203A, S207A, S212A

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
Myc-PSFΔRRM2

Plasmid

#35377

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 375-429

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pCIS.MTRIA-AGT

Plasmid

#184598

Purpose

For expression of a fluorescent sensor for angiotensin II in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-AGT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomKeima-SpHis5

Plasmid

#44847

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKeima with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKeima

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-cerMET10

Plasmid

#107440

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET10 (MET10 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-PSFΔRGG

Plasmid

#35185

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

N-terminal deletion of residues 9-27

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-eArch3.0-mRuby2-ST

Plasmid

#109135

Purpose

Archaerhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eArch3.0-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoClover-Kan

Plasmid

#44952

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Clover with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoClover

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-cerMET16

Plasmid

#107442

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET16 (MET16 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoLSS-mKate2-CaURA3

Plasmid

#44886

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-PSFΔRRM1

Plasmid

#35376

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 298-365

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
KA0717_pPB-hCMV*1-cHA-IRESVenus

Plasmid

#124168

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoTagRFP657-CaURA3

Plasmid

#44882

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFP657 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP657

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS011E: Entry vector for: superecliptic pHluorin (mitochondrial)

Plasmid

#163067

Purpose

Entry vector for: superecliptic pHluorin (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CeNL-H2B_pcDNA3

Plasmid

#89511

Purpose

Cyan color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Use

Tags

histone 2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GeNL(Ca2+)_250/pcDNA3

Plasmid

#85206

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_250

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomKO2-SpHis5

Plasmid

#44845

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKO2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKO2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1065_pPB-hCMV*1-cHA-IRESH2BmCherry

Plasmid

#124170

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBD21_037

Plasmid

#109154

Purpose

pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720

Depositor

Ron Weiss

Article

Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

Inserts

mRFP
iRFP720

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV and pTet

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
KA2601_eSpCas9-G2P

Plasmid

#124203

Purpose

Expression of eSpCas9(1.1) and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NLS-FUS-Citrine-PixE

Plasmid

#111506

Purpose

For the nuclear localization of the PixELL system

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

PixE (slr1693 )

Use

Lentiviral

Tags

Citrine, FUS, and NLS

Expression

Mutation

Promoter

SFFV

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomWasabi-Kan

Plasmid

#44945

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mWasabi with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomWasabi

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2-Katushka-9-5

Plasmid

#104011

Purpose

Plasmid encoding Katushka-9-5

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Katushka9-5

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (K6S, N21D, R32G, L…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomRuby-SpHis5

Plasmid

#44843

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomEos2-CaURA3

Plasmid

#44887

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mEos2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomEos2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPCX mCER-Prx1

Plasmid

#158227

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx1

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Gag-delta-p6-mEGFP

Plasmid

#116890

Purpose

expresses Gag missing the p6 domain, tagged with mEGFP, in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

synthetic Gag missing the p6 domain

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomKO2-Kan

Plasmid

#44958

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKO2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKO2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-PSFΔ+/-

Plasmid

#35378

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 575-611

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pRSET FLII12Pglu-600u

Plasmid

#13563

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A Y12S-ECFP-D13R

Use

Tags

Citrine

Expression

Bacterial

Mutation

F16A ECFP insertion in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPCX mCER-Prx2

Plasmid

#158225

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-Per

Plasmid

#33157

Depositor

Michael Rosbash

Article

Nawathean et al Mol Cell. 2004 Jan 30;13(2):213-23.

Insert

Per (per Fly)

Use

Retroviral

Tags

6xHIS and V5

Expression

Insect

Mutation

Promoter

Actin 5C

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
sfTq2-C1

Plasmid

#117932

Purpose

Superfolder mTurquoise2

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

sfTq2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH45

Plasmid

#125810

Purpose

Level0 nSpCas9-ABE7.10 base changer, human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nSpCas9-ABE7.10 base changer, human codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
BCJJ339

Plasmid

#117501

Purpose

RPS5a:Cas9:E9 Golden Gate Level 1 Position 2

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtRPS5a

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomApple-SpHis5

Plasmid

#44844

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mApple with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomApple

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pN_35S/mEGFP

Plasmid

#132565

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomApple-CaURA3

Plasmid

#44879

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mApple with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomApple

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH67

Plasmid

#131766

Purpose

Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoPA-mCherry-SpHis5

Plasmid

#44855

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-Clover

Plasmid

#103991

Purpose

Plasmid encoding Clover

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Clover

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHERRY8

Plasmid

#24663

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-PSFΔPQ

Plasmid

#35187

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 34-113

Promoter

CMV

Available since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only
RSV CREB M1

Plasmid

#22395

Depositor

Marc Montminy

Article

Gonzalez et al Cell. 1989 Nov 17. 59(4):675-80.

Insert

CREB (Creb1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Showing: 2201 - 2250 of 13557 results

Results per page:

Sort results by:

	More than 20 requests
	More than 50 requests
	More than 100 requests