We narrowed to 11,712 results for: NSI

1. pET28 His-MBP-TEV-BAP-Human BCKDK

   Plasmid

   #232116

   Purpose

   For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   BCKDK (BCKDK Human)
   

   Tags

   6 x His, Biotin acceptor peptide (BAP), Maltose-B…

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-CAG-Ace2N-4AA-mNeon-ST A122D-WPRE-bGH-polyA

   Plasmid

   #172912

   Purpose

   Mammalian expression of voltage sensor; soma localization targeting sequence

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Ace2N-4AA-mNeon-ST A122D
   

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)

   Plasmid

   #207357

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
   

   Use

   Lentiviral

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.23-CD69 CaRE3 (plus strand)

   Plasmid

   #91844

   Purpose

   Luciferase reporter for CD69 enhancer (IGI-P0621)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   CD69 CaRE3 (plus strand) (CD69 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Missing 1 base from 13 base polyT tract beginning…

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1α1.1-Archon1-KGC-GFP-ER2

   Plasmid

   #115890

   Purpose

   AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-GFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Promoter

   EF1a（1.1kb short version)

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXPR_BRD003 PSMB5 guide 1

   Plasmid

   #117073

   Purpose

   single guide RNA targeting PSMB5; guide 1

   Depositor

   William Hahn

   Article

   Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

   Insert

   LMPX (PSMB5 Human)
   

   Use

   CRISPR

   Promoter

   hU6

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAav-MDM2-PQS2-3xHA

   Plasmid

   #84886

   Purpose

   rAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette

   Depositor

   Sven Eyckerman

   Article

   Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

   Inserts

   MDM2 genomic sequence HR1 (MDM2 Human)
   MDM2 genomic insert HR2 (MDM2 Human)
   

   Use

   AAV

   Tags

   PQS2 3xHA

   Promoter

   no

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]

   Plasmid

   #115893

   Purpose

   AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Has Service

   AAV8

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Promoter

   Syn

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR1A_mmFbxw7(res) (closed)

   Plasmid

   #160105

   Purpose

   Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Insert

   Fbxw7alpha (Fbxw7 Mouse)
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1α1.1-FLEX-rc [Archon1-KGC-GFP-ER2]

    Plasmid

    #115891

    Purpose

    AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Promoter

    EF1α promoter (1.1kb short version)

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

    Plasmid

    #162878

    Purpose

    Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXPR_BRD003 PSMB5 guide 2

    Plasmid

    #117074

    Purpose

    single guide RNA targeting PSMB5; guide 2

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    LMPX (PSMB5 Human)
    

    Use

    CRISPR

    Promoter

    hU6

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.23-IL2RA CaRE2 (plus strand)

    Plasmid

    #91846

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0623)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE2 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    rs6602425 T>G, T>C at chr10:6117603

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. MB 38t3 thsS(t3)R-bARGSer

    Plasmid

    #232468

    Purpose

    Optimized thiosulfate sensor with acoustic reporter genes (bARGSer)

    Depositor

    Mikhail Shapiro

    Article

    Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:

    Inserts

    thsS(t3)
    thsR
    PphsA342
    bARGSer
    AxeTxe
    

    Expression

    Bacterial

    Mutation

    Contains the following mutations K286Q, Q350H, I…

    Promoter

    J23104 (ttgacagctagctcagtcctaggtattgtgctagc), J23…

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28 MBP-TEV-Rat BCKDK-His

    Plasmid

    #232119

    Purpose

    For bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    BCKDK (Bckdk Rat)
    

    Tags

    6 x His, Maltose-Binding Protein (MBP), and Tev S…

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-PB2_Lock1-I436C+S993C

    Plasmid

    #182879

    Purpose

    Lentivirus for expression of Plexin-B2 locked ring mutant

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2025 Jan 2;16(1):272. doi: 1

    Insert

    hPLXNB2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Mutation

    changed Isoleucine 436 to Cysteine and Serine 993…

    Available Since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-PB2_Lock2-I436C+T1051C

    Plasmid

    #182880

    Purpose

    Lentivirus for expression of Plexin-B2 locked ring mutant

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2025 Jan 2;16(1):272. doi: 1

    Insert

    hPLXNB2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Mutation

    changed Isoleucine 436 to Cysteine and Threonine …

    Available Since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTL

    Plasmid

    #203176

    Purpose

    Centromeric yeast expression vector, leucine selection

    Depositor

    William Sessa

    Article

    Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX304_zeo_mmFbxw7(res)

    Plasmid

    #160106

    Purpose

    Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Fbxw7alpha (Fbxw7 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXPR_BRD003 UBE2C guide 1

    Plasmid

    #117068

    Purpose

    single guide RNA targeting UBE2C; guide 1

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    UBCH10 (UBE2C Human)
    

    Use

    CRISPR

    Promoter

    hU6

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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