We narrowed to 12,197 results for: 112

1. CoV2-N-P13L

   Plasmid

   #177946

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (P13L) (N )
   

   Expression

   Mammalian

   Mutation

   P13L

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CoV2-N-P80R

   Plasmid

   #177947

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (P80R) (N )
   

   Expression

   Mammalian

   Mutation

   P80R

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CoV2-N-S235F

   Plasmid

   #177956

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (S235F) (N )
   

   Expression

   Mammalian

   Mutation

   S235F

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Arp3_pLib

   Plasmid

   #173677

   Purpose

   Arp3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

   Depositor

   Roberto Dominguez

   Article

   Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

   Insert

   Arp3 (ACTR3 Human)
   

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4-Tet-on-vsv-Incenp-EGFP

   Plasmid

   #108483

   Purpose

   inducible expression of vsv-INCENP wt-EGFP

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   INCENP (INCENP Human)
   

   Tags

   EGFP and VSV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV10/3xflag-Clock L57E

   Plasmid

   #47337

   Purpose

   Mutation used for co-IPs

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   L57E

   Promoter

   CMV

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pMXs_FLAG-ODC2

   Plasmid

   #133257

   Purpose

   Express FLAG-tagged ODC2 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   ODC2 (ODC2 Budding Yeast)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in human cells

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs_FLAG-NDT2

   Plasmid

   #133255

   Purpose

   Express FLAG-tagged NDT2 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   NDT2 (YEA6 Budding Yeast)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in human cells

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

   Plasmid

   #82469

   Purpose

   Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   mRNA capping enzyme (Rngtt Mouse)
   

   Tags

   Flag and Myc

   Expression

   Mammalian

   Mutation

   changed Lysine 294 to Alanine

   Promoter

   CMV

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4/TO-myc NES-mCE NLS-Flag

    Plasmid

    #82468

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    Myc-NES-mCE-Flag (Rngtt HIV, Mouse)
    

    Tags

    Flag and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV10/3xflag-Clock L74E

    Plasmid

    #47338

    Purpose

    Mutation used for co-IPs

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    L74E

    Promoter

    CMV

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5/FRT/TO/SH/GW-Mst1

    Plasmid

    #84299

    Purpose

    Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.

    Depositor

    Jonathan Chernoff

    Article

    Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71

    Insert

    Mst1 (STK4 Human)
    

    Tags

    HA, SBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pRAN357

    Plasmid

    #120898

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

    Insert

    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS315-0nt-telo-I-SceI

    Plasmid

    #67624

    Purpose

    telomere cassette for telomere formation system containing 0 bp of S. pombe telomere repeats

    Depositor

    Kurt Runge

    Article

    Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

    Inserts

    3' gal1 unique sequence
    ura4 (ura4 Fission Yeast)
    telomere cassette and I-SceI cut site
    hph
    

    Use

    Yeast cen/ars plasmid

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQTEV-TCTEL1

    Plasmid

    #31337

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    t-complex-associated-testis-expressed 1-like 1 (DYNLT1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWPT-/UAA-mEGFP-IRES-mCherry

    Plasmid

    #49234

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Promoter

    EF1-short

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-dLbCpf1-BE0ΔiNLS

    Plasmid

    #107684

    Purpose

    Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE0ΔiNLS
    

    Expression

    Mammalian

    Mutation

    D832A/E925A/D1148A

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRII-Topo U6-SpAi9L-U6SpDMDL

    Plasmid

    #78608

    Purpose

    Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9L-U6SpDMDL
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pCRII-Topo U6-SpAi9R-U6-SpDMDR

    Plasmid

    #78609

    Purpose

    Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9R-U6-SpDMDR
    

    Use

    Unspecified

    Promoter

    U6

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pLJM1-zeo-ORF66-2xStrep

    Plasmid

    #130957

    Purpose

    Lentiviral vector for expression of KSHV ORF66 with a C-terminal 2xStrep tag

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Lentiviral

    Tags

    2xStrep

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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