We narrowed to 30,760 results for: PLE;

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pRK7-FLAG-TSC2

Plasmid

#8996

Depositor

John Blenis

Article

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC2 (TSC2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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P1-EYFP-pA (Construct 5)

Plasmid

#55197

Purpose

Synthetic promoter responsive to gRNA1

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

EYFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

Minimal Ade MLP

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pMSP3545-dCas9Str

Plasmid

#153516

Purpose

Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

dCas9Str

Expression

Bacterial

Promoter

nisA

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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HP138-neo

Plasmid

#134247

Purpose

Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selection

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

Contains Cas9

Use

CRISPR

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Pediculus humanus corporis PINK1 (115 - 575)

Plasmid

#110750

Purpose

Bacterial expression of PhPINK1 (biochemistry)

Depositor

David Komander

Article

Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

Insert

Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)

Tags

N-His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-MCS

Plasmid

#75174

Purpose

Multiple cloning site for insertion of gene of interest for C-terminal fusions.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

Multiple Cloning Site

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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F-Cdk8

Plasmid

#15366

Depositor

Joan Conaway, Ronald Conaway

Article

Conaway et al Trends Biochem Sci. 2005 May . 30(5):250

Insert

CDK8 (CDK8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA-miRFP718nano

Plasmid

#195744

Purpose

Monomeric near-infrared fluorescent protein miRFP718nano in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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LentiAsCpf1-3xMYC-blast

Plasmid

#127750

Purpose

Lentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal

Depositor

Giulio Draetta

Article

Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

Insert

AsCpf1

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-NLS-3HA-KanMX6

Plasmid

#228022

Purpose

To integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-NLS-3HA-KanMX6

Plasmid

#228026

Purpose

To integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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(SUMO)10

Plasmid

#126948

Purpose

Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Plasmid

#163075

Purpose

Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

roGFP2-Orp1

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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Cyto-ABKAR

Plasmid

#61510

Purpose

Expression of AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pDGE508

Plasmid

#153280

Purpose

End-linker for cloning 2 sgRNA arrays into any recipient vector

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Insert

linker

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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p3x-FLAG-hnRNPF

Plasmid

#21926

Depositor

Mariano Garcia-Blanco

Article

Mauger et al Mol Cell Biol. 2008 Sep . 28(17):5403-19

Insert

hnRNPF (HNRNPF Human)

Tags

3 Flags

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pZW1-FCS-circRNA

Plasmid

#73449

Purpose

Expression of circular RNA

Depositor

Ling-Ling Chen

Article

Zhang et al Cell. 2014 Sep 25;159(1):134-47. doi: 10

Insert

POLR2A (POLR2A Human)

Tags

none

Expression

Mammalian

Mutation

none

Promoter

CMV promoter

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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NbV5-HALOTAG

Plasmid

#201482

Purpose

Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-HALOTAG

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pYL-kasOp-FnCas12a3

Plasmid

#177875

Purpose

Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

Depositor

Yunzi Luo

Article

Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

Insert

Streptomyces codon optimized the FnCas12a mutant EP16

Use

CRISPR

Promoter

kasOp*

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ran-T24N-mRFP1-polyA

Plasmid

#104561

Purpose

Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran (Ran Mouse)

Tags

mRFP1

Expression

Mammalian

Mutation

T24N

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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