We narrowed to 17,736 results for: EMB
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Plasmid#106377PurposeC. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposonDepositorInsertPtet-himar1-Ter(slpA)-transposon
UseE. coli - c. difficile shuttle vectorMutationCodon optimised for C. difficilePromoterPtetAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lyso-GA
Plasmid#209874PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddGFP A
TagsLAMP1ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PM-B
Plasmid#209876PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddGFP B
TagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
LD-B
Plasmid#209861PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddGFP B
TagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-CAAX
Plasmid#116856Purposeplasma membrane markerDepositorInsertHRAS
TagsTagBFP2ExpressionMammalianMutationAmino acids 172-189Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV_Stargazin-mTurquoise2-iLID
Plasmid#161001PurposeMammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusionDepositorInsertStargazin-mTurquoise2-iLID
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lyso-RA
Plasmid#209875PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddRFP A
TagsLAMP1ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Bpa
Plasmid#155342PurposePlasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.DepositorInserthumanized EBpaRS/BstYam
ExpressionMammalianPromoterPGK/U6Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEx-insertion-tet
Plasmid#172211PurposeFor genetic insertion in tetracyclin-sensitive Prevotella copri strainsDepositorTypeEmpty backboneUseGenetic insertion in prevotella copriAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PM-RA
Plasmid#209878PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddRFP A
TagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGene-TAP-GBP1
Plasmid#138087PurposePlasmid with TAP-GBP1 ORFDepositorInserthuman GBP1 (GBP1 Human)
TagsTandem affinity purification (TAP) tagExpressionMammalianPromoterGal4-E1bAvailable SinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
HRE-doUnaG
Plasmid#201713PurposeHypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequenceDepositorInsertUnaG
ExpressionMammalianAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
LD-GA
Plasmid#209862PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddGFP A
TagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
LD-RA
Plasmid#209863PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddRFP A
TagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PM-GA
Plasmid#209877PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddGFP A
TagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human Parkin (1-465)
Plasmid#110758PurposeBacterial expression of human ParkinDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-PSMA
Plasmid#11524DepositorInsertprostate-specific membrane antigen (FOLH1 Human)
TagsHisExpressionInsectMutationThere is a short N-terminal truncation of this pr…Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP11_pcDNA3.1(-)
Plasmid#182932PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragmentDepositorTypeEmpty backboneTags3C-cleavable split GFP 11, FLAG tag, and HA signa…ExpressionMammalianPromoterCMVAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR4-Kras
Plasmid#61621PurposeNon-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activityDepositorInsertAKAR4-Kras
TagsC-terminal targeting sequence from Kras, Cerulean…ExpressionMammalianPromoterCMVAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
TPC1-G-GECO1.2
Plasmid#207143PurposeFusion of Ca2+ reporter and endosomal Ca2+-permeable channelDepositorAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA
Plasmid#230988PurposeCo-expressing membrane-localized Voltron2 and membrane-localized CheRiff.DepositorInsertCre-dependent, membrane-localized optopatch for neuronal dendrites
UseAAVExpressionMammalianPromoterhSynAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-LMP1-IRES-eGFP
Plasmid#36955DepositorAvailable SinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E3D1_D73C
Plasmid#39328DepositorInsertMSP1E3D1_D73C (APOA1 synthetic gene)
Tags6xHisExpressionBacterialMutationcys mutant of MSP1E3D1PromoterT7Available SinceSept. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOPS0359
Plasmid#115508PurposeReporter Plasmid. Taurine-inducible expression of GFPDepositorInsertsfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
ExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterTaurine-inducible promoter of S. meliloti Promote…Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV_mTurquoise2-iLID-CAAX
Plasmid#160999PurposeMammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via C-terminal fusion to CAAX motif from KRASDepositorInsertmTurquoise2-iLID-CAAX
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_EPHB4
Plasmid#187768PurposeMAC-tagged gene expression of human EPHB4DepositorInsertEPHB4 (EPHB4 Human)
ExpressionMammalianAvailable SinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPS0377
Plasmid#115509PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFPDepositorInsertsfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSP1E3D1
Plasmid#199772PurposeExpresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)DepositorInsertcMSP1E3D1
TagsHis6-TagExpressionBacterialMutationExtended cMSP1D1; external His-Tags flanking the …Available SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEx-deletion-erm
Plasmid#172213PurposeFor allelic exchange in erythromycin-sensitive Prevotella copri strainsDepositorTypeEmpty backboneUseAllelic exchange in prevotella copriAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
BB-NIC-II-HisN-TET12
Plasmid#45496DepositorInsertTET12
UseSynthetic BiologyTags6xHistidine tagExpressionBacterialPromoterT7Available SinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTM-BPL
Plasmid#173766PurposeFluorescent labeling of the nuclear envelopeDepositorInsertPDGF receptor TM domain, Biotin protein ligase
TagsFLAG and Signal peptideExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBCCP-GFP-NLS
Plasmid#173923PurposeFluorescent labeling of the nuclear envelopeDepositorInsertBiotin carboxyl carrier protein, AcGFP1
Tags3xNLSExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP1-10_pcDNA3.1(-)
Plasmid#182931PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragmentDepositorTypeEmpty backboneTags3C-cleavable split GFP 1-10, FLAG tag, and HA sig…ExpressionMammalianPromoterCMVAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTH761-CEN-mCherry_v4
Plasmid#40599DepositorInsertmCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
ExpressionYeastMutationmCherry ORF partially codon optimised for yeast e…PromoterGPD (TDH3)Available SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_INSR
Plasmid#187772PurposeMAC-tagged gene expression of human INSRDepositorInsertINSR (INSR Human)
ExpressionMammalianAvailable SinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNDGG054
Plasmid#231323PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG010, pOGG012, and pOGG014, NmR/KmRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
5HT6-YFP-Inp54p
Plasmid#96812Purposecilia-membrane-targeted inositol polyphosphate 5-phosphataseDepositorInsert5HT6-YFP-Inp54p
TagsYFPExpressionMammalianAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-mGFP-pA(AV222)
Plasmid#117858PurposeThis plasmid expresses membrane anchored GFP to facility axon morphology study.DepositorInsertmGFP
UseAdenoviralExpressionMammalianPromoterCMVAvailable SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
ss-SigmaR1DTM-mNeonGreen
Plasmid#226575PurposeEncodes mutant SigmaR1 protein (transmembrane region deletion) labeled with mNeonGreenDepositorAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Opto-ACTU−
Plasmid#198869PurposeOptogenetic recruitment construct to increase inner membrane surface chargeDepositorInsertRad17 polyanionic tail (-14)
UseSynthetic BiologyTagsCRY2PHR-mCherryExpressionMammalianPromoterCMVAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_INSR
Plasmid#187788PurposeMAC-tagged gene expression of human INSRDepositorInsertINSR (INSR Human)
ExpressionMammalianAvailable SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV_ADRB2-mTurquoise2-iLID
Plasmid#161002PurposeMammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusionDepositorInsertADRB2-mTurquoise2-iLID
UseLentiviralTagsFLAGExpressionMammalianPromoterEF1aAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEx-insertion-erm
Plasmid#172210PurposeFor genetic insertion in erythromycin-sensitive Prevotella copri strainsDepositorTypeEmpty backboneUseGenetic insertion in prevotella copriAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_LMTK3
Plasmid#187773PurposeMAC-tagged gene expression of human LMTK3DepositorInsertLMTK3 (LMTK3 Human)
ExpressionMammalianAvailable SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTagBFP2-C1-lynN11-FKBP-PI-PLCN187
Plasmid#139308PurposeN-terminal half of split PI-PLC targeted to plasma membraneDepositorInsertLYN(1-11):RSANS[GA]4:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)
ExpressionMammalianAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
5HT6-YFP-PIPK
Plasmid#96810Purposecilia-membrane-targeted phosphatidylinositol 4-phosphate 5-kinaseDepositorInsert5HT6-YFP-PIPK
TagsYFPExpressionMammalianAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pNDGG003
Plasmid#231316PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2850
Plasmid#87758PurposePcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.DepositorInsertPcol-19-PH-miniSOG(Q103L)
TagsPHExpressionWormMutationQ103LPromoterPcol-19Available SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pinco-mNrp1
Plasmid#21937DepositorAvailable SinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only