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Plasmid#90109PurposeMammalian expression of Flag-MCPIP1(150-453)DepositorInsertMCPIP1 (ZC3H12A Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-Flag-mIL-17RA/CFP.Hygro
Plasmid#46861Purposeencodes Flag-tagged mouse IL-17RA fused to Cyan Fl. ProteinDepositorInsertIL-17RA-CFP
UseTagsFlag/IL-3 signal sequence and fused to CFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialMutationPromoterPydfJ115Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML RING
Plasmid#185631Purposeexpression of GST-tagged PML RING domain in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationnonePromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML RB1
Plasmid#185636Purposeexpression of GST-tagged PML fragment 49-168 (RING + B-box1) in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationnonePromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
mFzd4CRDL(42-203)-6xHis_pAcGP67a
Plasmid#216379PurposeBaculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cellsDepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTags6xHis and GP64 signal sequenceExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PGUS
Plasmid#207827PurposeFLAG-tagged PGUS driven by CMV promoterDepositorInsertPGUS
UseLentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-peripherin-CTS
Plasmid#86070PurposeA bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.DepositorInsertPeripherin (prph2.S Frog)
UseTagsGSTExpressionBacterialMutationcontains the region from 317-336 of peripherinPromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-prRDH-CTS
Plasmid#86069PurposeA bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.DepositorInsertRetinol dehydrogenase (RDH8 Bovine)
UseTagsGSTExpressionBacterialMutationthe insert is 297-312 of RDH8 (Bos taurus)PromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj12-o11
Plasmid#69941PurposepSTC2,theoHHAzRaj12,cisRaj11-sfGFP, KanRDepositorInsertTheoHHAzRaj12,cisRaj11,sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj12-olsa
Plasmid#69940PurposepSTC2,theoHHAzRaj12,cisRaj12-sfGFP, KanRDepositorInsertsTheoHHAzRaj12,cisRaj12,sfGFP
theoHHAzRaj12,cisRaj12-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-I70V/D91G/V220A
Plasmid#47647PurposepAC-EsaR with a208g, a272g, c659t mutations in esaRDepositorInsertesaR-I70V/D91G/V220A
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Isoleucine 70 to Valine, Aspartic acid 91…PromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2 YFP-Smo-Weyer'sAla5
Plasmid#41016DepositorInsertsmoothened homolog (Smo Mouse)
UseTagsEVC2 ala5 (aa 1177-1220 with amino acids 1185-118…ExpressionMammalianMutationYFP inserted after Smo signal sequence. Transfer…PromoterAvailable sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-STAT3
Plasmid#111934PurposeExpresses human STAT3 fused to GFP in mammalian cellsDepositorInsertSignal transducer and activator of transcription 3 (STAT3 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-STAT1-mGFP-P2A-Puro
Plasmid#199345Purposeexpress murine STAT1 protein fused with mGFPDepositorInsertsignal transducer and activator of transcription 1 (Stat1 Mouse)
UseLentiviralTagsmGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSIE1
Plasmid#136355PurposeSelection by Inducible EffectorsDepositorInsertsType VI effector bfe1
Type VI effector bte1
BT_4676
tetR
UseUnspecifiedTagsExpressionMutationATG removed to add signal sequence before genePromoterP1TDP-GHO23Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX304-VHL-IRES-GFP
Plasmid#187641PurposeVHL Expression vector with GFPDepositorInsertVHL (VHL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp PHAkt Cerulean
Plasmid#50837PurposeFluorescent reporter for membrane PIP3 levelsDepositorInsertPHAkt (AKT1 )
UseLentiviral and Synthetic BiologyTagsCeruleanExpressionMutationPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO
Plasmid#204341PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-i3/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-NRF3
Plasmid#207815PurposeN-terminal tagged NRF3 for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-NRF3 (NFE2L3 Human)
UseLentiviralTagsminiTurbo-V5ExpressionMutationPromoterUBCAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only