We narrowed to 27,275 results for: gfp gene

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pHAGE-FKBP-GFP-NDP52

Plasmid

#135296

Purpose

stably express GFP-tagged NDP52 for CID induced mitophagy

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

NDP52 (CALCOCO2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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PWPXLD-GFP-Numb-S276D

Plasmid

#133752

Purpose

Numb with mutation at serine 276 converted to aspartic acid cand tagged with GFP cloned into PWPXLD

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

NUMB endocytic adaptor protein (Numb Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

serine 276 converted to aspartic acid

Promoter

EF1-alpha

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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FU_tetO_dCas9-VP192-T2A-EGFP

Plasmid

#121173

Purpose

Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

Depositor

Timo Otonkoski

Insert

dCas9-VP192-T2A-EGFP

Use

CRISPR and Lentiviral; Inducible by doxycycline

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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AAV EGFP donor pDY0404

Plasmid

#219862

Purpose

AAV EGFP donor

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

EGFP

Use

AAV

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1(T17N)

Plasmid

#13721

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(T17N) (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT-TO-EGFP-UPF1-WT

Plasmid

#136000

Purpose

DOX-inducible WT UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-P2A-EGFP_mRFPstuf

Plasmid

#137730

Purpose

Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA

Depositor

Fredrik Wermeling

Article

Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

Type

Empty backbone

Use

CRISPR, Lentiviral, Mouse Targeting, and Syntheti…

Expression

Bacterial and Mammalian

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-GFP-V5-His

Plasmid

#85621

Purpose

Expresses GFP and V5 in Drosophila.

Depositor

Kenneth Irvine

Article

Rauskolb et al PLoS Biol. 2011 Jun;9(6):e1000624. doi:

Insert

GFP

Tags

V5, His

Expression

Insect

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-CD59

Plasmid

#222307

Purpose

Synchronize the trafficking of CD59 from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and CD59 fused to SBP-EGFP (CD59 Human)

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-6)-H2_mCherry(-6)

Plasmid

#205049

Purpose

Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H2_mCherry(-6)

Tags

MGHHHHHGGA

Expression

Bacterial

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-EGFP

Plasmid

#65258

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-MRLC T18A,S19A

Plasmid

#35681

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T18A and S19A

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pPB_TRE3G_scFV-sfGFP-Ezh2FL-CatMut

Plasmid

#235584

Purpose

Dox-inducible expression of control catalytic-mutant Ezh2 fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ezh2 (Ezh2 Mouse)

Use

CRISPR

Mutation

Y726D

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDD317 (Pmyo-2::GFP + SEC)

Plasmid

#73344

Purpose

SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

Insert

Pmyo-2::GFP + SEC

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Promoter

myo-2

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a-EGFP (no signal control)

Plasmid

#170167

Purpose

Mammalian Expression of no signal control

Depositor

Christopher Moxham

Type

Empty backbone

Expression

Mammalian

Mutation

n/a

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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HA, EGFP Spastin M87 with exon 4

Plasmid

#167542

Purpose

For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4.

Depositor

Tarun Kapoor

Article

Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

Insert

Spastin M87 with exon 4 (SPAST Human)

Tags

EGFP and HA tag

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pKrox24(MapERK)d1EGFP (KroxDs)

Plasmid

#214912

Purpose

for PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activation

Depositor

Pavel Krejčí

Article

Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

Insert

d1EGFP under the MapERK promoter

Expression

Mammalian

Promoter

MapkERK promoter

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-HALO-NTERM-GFP

Plasmid

#185762

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, Halo

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHRm-GFP(1-10)-VP64-NLS

Plasmid

#70228

Purpose

Expresses GFP(1-10)-VP64-NLS in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP(1-10)-VP64-NLS

Use

Lentiviral

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CIBN-EGFP-KRasCT

Plasmid

#178527

Purpose

Expresses CIBN-EGFP-KRasCT in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

CIBN-EGFP-KRasCT

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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