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pCR4-CTE

Plasmid

#36868

Depositor

Mario Chin

Insert

CTE

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBS-mPax3

Plasmid

#16427

Depositor

Jonathan Epstein

Insert

Pax3 (Pax3 Mouse)

Use

In situ template

Mutation

None

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pHDR_sox2HA_P2A-GFP-pA_HA_G2913A

Plasmid

#196191

Purpose

Tagging human SOX2 gene with P2A-EGFP at the C-terminus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

SOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)

Use

Homology repair donor

Mutation

G2913A mutation to replace the guide cutting site…

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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PB-PG13-mCherry-EF1a-PuroR-EGFP

Plasmid

#195292

Purpose

piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

mCherry
PuroR-P2A-EGFP

Use

Piggybac transposon

Expression

Mammalian

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSB118 - pL2_pSB90_fLUC-I_HcPro

Plasmid

#123193

Purpose

binary plant vector for transient TEV HcPro and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP-KCC2

Plasmid

#104075

Purpose

expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

Depositor

Igor Medyna

Article

Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

CMV

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDB5320

Plasmid

#204830

Purpose

An integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

Depositor

Li-Lin Du

Article

Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

Inserts

tetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW2 promoter

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Fra-2

Plasmid

#187907

Purpose

Expresses Fra-2 in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

Fra2 (Fosl2 Mouse)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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ePB-PURO-TT-PA-CRE

Plasmid

#179584

Purpose

ePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

PA-MAG-CRE

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Rap1

Plasmid

#134928

Purpose

Fluorescently tagged GTPase.

Depositor

Klaus Hahn

Article

O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

Insert

Rap1B, Cerulean

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-EGFP

Plasmid

#165040

Purpose

Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

EGFP

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1

Plasmid

#134301

Purpose

Bacterial expression of inverse-response citrate sensor with high binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-HF

Plasmid

#127882

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3F tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-Sun

Plasmid

#119858

Purpose

Cmv d0 dPspCas13b-GGS-Sun no-fusion construct

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-Sun

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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HyGCaMP6s

Plasmid

#102558

Purpose

Expression of GCaMP6s in Hydra

Depositor

Rafael Yuste

Article

Dupre et al Curr Biol. 2017 Apr 24;27(8):1085-1097.

Insert

GCaMP6s

Use

Hydra expression

Tags

Actin/GCaMP6s fusion protein (C terminal on backb…

Available Since

Oct. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pDUP3

Plasmid

#92174

Purpose

Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.

Depositor

Judith Berman

Article

Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

Insert

NAT1

Use

Genbank: u03440.1

Expression

Yeast

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CIBN-CreC

Plasmid

#75267

Purpose

The first half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

N-terminal portion of the CIB1 transcription factor

Use

AAV

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB416

Plasmid

#74810

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB507

Plasmid

#74849

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR5'-attP (pCM254)

Plasmid

#68311

Purpose

Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

Depositor

Christian Mosimann, Leonard Zon

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Insert

attP

Use

Gateway Cloning

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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