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pcDNA3.3-Nm-dCas9-p300 Core

Plasmid

#61365

Purpose

encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Insert

Nm-dCas9-p300 Core (EP300 Human, Synthetic, Neisseria meningiditis)

Use

CRISPR and Synthetic Biology

Tags

HA tag and NLS

Expression

Mammalian

Mutation

D16A, D587A, H588A, N611A in Nm Cas9

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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Integrin-Alpha2-N-18

Plasmid

#58192

Purpose

Localization: Integrin/Focal Adhesions, Excitation: N/A, Emission: N/A

Depositor

Michael Davidson

Insert

Integrin-Alpha2 (ITGA2 Human)

Tags

SNAP tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
USP15

Plasmid

#28282

Depositor

Cheryl Arrowsmith

Insert

USP15 (USP15 Human)

Tags

His tag with thrombin cleavage

Expression

Bacterial

Mutation

Contains dusp and Ubl domains (residues 6-223).

Available Since

April 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits
TFORF3397

Plasmid

#144873

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZIK1 (ZIK1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-CANX

Plasmid

#227279

Purpose

Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CANX (CANX Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

Plasmid

#135814

Purpose

Fluorescent reporter for knock-in of TdTomato into the TH locus

Depositor

Tim Ahfeldt, Lee Rubin

Article

Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

Insert

TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)

Tags

p2a-TdTomato

Expression

Mammalian

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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DsRed-rab9 WT

Plasmid

#12677

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Tags

DsRed and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ WT

Plasmid

#24809

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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T7-Elongin C-pcDNA3

Plasmid

#19998

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

Elongin C (ELOC Human)

Tags

T7-tag

Expression

Mammalian

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR223_SLIRP_WT

Plasmid

#82288

Purpose

Gateway Donor vector containing SLIRP, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SLIRP (SLIRP Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-CD36-C-10

Plasmid

#55280

Purpose

Localization: Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

CD36 (CD36 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
(SIM)10

Plasmid

#126946

Purpose

Bacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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TRX1 flag C32S

Plasmid

#21284

Depositor

Wang Min

Article

Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

Insert

TRX1 (TXN Human)

Tags

Flag

Expression

Mammalian

Mutation

C32S

Available Since

July 30, 2009

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ERBB2 A775_G776insV, G776C

Plasmid

#40979

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

A775_G776insV, G776C

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7B2Jnk2a2

Plasmid

#19727

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

pCDNA3 FlagMKK7B2Jnk2a2 (MAPK9, Map2k7 Human, Mouse)

Tags

Flag

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
PLKO.1-G3BP1-CDS

Plasmid

#136039

Purpose

G3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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CRY2 mCherry Sec61B

Plasmid

#102246

Purpose

Expresses fusion of CRY2PHR with mCherry and Sec61B

Depositor

Bianxiao Cui

Article

Che et al ACS Synth Biol. 2015 Oct 16;4(10):1124-3

Insert

CRY2PHR-mCherry-SEC61beta (CRY2 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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GST-AR-1-660

Plasmid

#104196

Purpose

Expresses human AR-1-660 from bacterial expression vector pGEX-5X-1 as GST fusion protein

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-660 (AR Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pHybrid-Electra1

Plasmid

#179474

Purpose

Expresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Inserts

Electra1
mScarlet-I

Expression

Bacterial and Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT

Plasmid

#134645

Purpose

lysosome-targeted Sac1 catalytic domain wild type

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

Sac1 catalytic domain (77-520 aa) (SACM1L Human)

Use

Lentiviral

Tags

FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…

Expression

Mammalian

Mutation

contains only amino acids 77-520 (Please see depo…

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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