We narrowed to 9,906 results for: UTY

1. pLenti-VRER-P2A-Puro

   Plasmid

   #110849

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-HF1-P2A-Puro

   Plasmid

   #110850

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-VQR-PGK-Puro

   Plasmid

   #110855

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQR
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-VRER-PGK-Puro

   Plasmid

   #110856

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX302 PCSK5 (T288P)-V5 puro

   Plasmid

   #98709

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Threonine 288 to Proline

   Promoter

   Available Since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-2B-Nkx2-5YRD Y-A

   Plasmid

   #98611

   Purpose

   Gateway entry vector for NKX2-5 YRD Y-A

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   NKX2-5 YRD Y-A (Nkx2-5 Mouse)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Substitutions of 7 tyr into Ala residues.Y233A; Y…

   Promoter

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR1A DHFR.dn-cHSF1

   Plasmid

   #134726

   Purpose

   Gateway entry vector for constitutively active dominant-negative HSF1 fused to the DHFR destabilized domain

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

   Insert

   DHFR.dn-cHSF1 (HSF1 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   DHFR

   Expression

   Mutation

   Promoter

   None

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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8. CRISPR-SB

   Plasmid

   #177936

   Purpose

   Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

   Depositor

   Roland Rad

   Article

   Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

   Type

   Empty backbone

   Use

   CRISPR; Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2-hUbC-dCas9-KRAB-T2a-Puro

   Plasmid

   #225999

   Purpose

   To establish stable dCas9-KRAB knock-in line

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   humanized dCas9-KRAB T2A Puro
   

   Use

   Sleeping beauty transposon

   Tags

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xEcoLeuT(CUA)_AnapRS

    Plasmid

    #140019

    Purpose

    Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EcoLeuRS (AnapRS)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

    Promoter

    EF1

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-CMV 3xFLAG-GABARAPL1-GFP

    Plasmid

    #123112

    Purpose

    Expresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-CMV 3xFLAG-GABARAPL2-GFP

    Plasmid

    #123113

    Purpose

    Expresses 3xFLAG-GABARAPL2-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL2

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV 3xFLAG-GABARAPL2 G116

    Plasmid

    #123105

    Purpose

    Expresses 3xFLAG-GABARAPL2 G116 in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deleted amino acid 117. Stop codon after G116

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

    Plasmid

    #62577

    Purpose

    Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. mCherry-MUTHY

    Plasmid

    #215132

    Purpose

    Expresses mCherry-MUTHY in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    MUTHY (MUTYH Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-CMV GFP-GABARAPL1 G116

    Plasmid

    #123119

    Purpose

    Expresses EGFP-GABARAPL1 G116 in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acid 117. Stop codon after G116

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)

    Plasmid

    #62579

    Purpose

    Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

18. BM00369

    Plasmid

    #155338

    Purpose

    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. FSHR-DuET

    Plasmid

    #213242

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    FSHR (FSHR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. BM00654

    Plasmid

    #155339

    Purpose

    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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