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ABE8.17-m

Plasmid

#136298

Purpose

expresses ABE8.17-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.17-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a_CD WT TET1

Plasmid

#124082

Purpose

Expression of wild-type catalytic domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 catalytic domain (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF1 alpha

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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SPHK1 gRNA (BRDN0001147534)

Plasmid

#76814

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK1 (SPHK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SPHK2 gRNA (BRDN0001146882)

Plasmid

#77096

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK2 (SPHK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SPHK1 gRNA (BRDN0001148403)

Plasmid

#76815

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK1 (SPHK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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nCas9-PPE

Plasmid

#140445

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

Insert

nCas9(H840A)-M-MLV

Use

CRISPR

Expression

Plant

Mutation

H840A for Cas9; D200N, T306K, W313F, T330P and L…

Promoter

maize Ubiquitin-1

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pX458_Ruby

Plasmid

#110164

Purpose

Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

Depositor

Philip Hublitz

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

hSpCas9

Use

CRISPR

Tags

3xFLAG and Ruby2

Expression

Mammalian

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMK322 (TOP2A-mAC (Hygro))

Plasmid

#140653

Purpose

TOP2A tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

TOP2A (TOP2A Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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hCCR5-mTFP1

Plasmid

#110193

Purpose

Encodes for human CCR5 coding sequence tagged with the mTFP1 FP

Depositor

Sergi Padilla Parra

Article

Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

Insert

hCCR5 (CCR5 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-HP1alpha

Plasmid

#179703

Purpose

visualization of centromere heterochromatin

Depositor

Kenji Sugimoto

Article

Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

Insert

heterochromatin protein alpha (CBX5 Human)

Tags

mCherry

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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SFFV-DTAG-NTERM-GFP

Plasmid

#185760

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, dTAG

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3B-BE3

Plasmid

#113411

Purpose

Expresses hA3B-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3B-BE3 (APOBEC3B Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

Plasmid

#89508

Purpose

Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

Insert

rAPOBEC1-nCas9 (D10A)-UGI-NLS

Tags

UGI and rAPOBEC-XTEN

Expression

Bacterial

Mutation

D10A mutation of Cas9

Promoter

T7

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pFP5380 LAMP1-SBP- mCherry-FIREtag

Plasmid

#216725

Purpose

Expresses LAMP1-mCherry-FIREtag in mamallian cells

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

LAMP-1 (LAMP1 Human)

Tags

FIREtag, Streptavidin Binding Protein (SBP), and …

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadCBEd-BlastR

Plasmid

#193848

Purpose

Express TadCBEd in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Flag-cMyc T58A

Plasmid

#20076

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

cMyc (MYC Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

T58A

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRCH-YE1-BE4max

Plasmid

#198549

Purpose

Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-ERT2-iCre-ERT2

Plasmid

#178060

Purpose

Conditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.

Depositor

Edward Boyden

Article

Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ERT2-iCre-ERT2

Use

AAV

Expression

Mammalian

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Oct. 9, 2022

Availability

Academic Institutions and Nonprofits only

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A*68:01:01

Plasmid

#203292

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*68:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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A*02:11:01

Plasmid

#203280

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*02:11:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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