We narrowed to 2,097 results for: Pam

1. pLenti-mEtfdh-AAA4

   Plasmid

   #232316

   Purpose

   Expression of mouse mutant Etfdh (AAA4)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   Etfdh (Etfdh Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   PAM Sequence Mutations: (G36C; T39C, C42T; C72T, …

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-mEtfdh

   Plasmid

   #232314

   Purpose

   Expression of mouse mutant Etfdh

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   Etfdh (Etfdh Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   PAM Sequence Mutations: (G36C, T39C, C42T, C72T, …

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD649-HAsp-F11R-COMP5AP-AviTag-9xHis

   Plasmid

   #157196

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   F11R (F11R Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pD649-HAsp-F11R-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156632

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   F11R (F11R Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXS-IRES-BLAST flag-CAD 3E@3R

   Plasmid

   #188149

   Purpose

   Expresses C-terminal flag-tagged human CAD 3E@3R in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXS-IRES-BLAST flag-CAD R1398A A1400E R1403A R1404A L1405A

   Plasmid

   #188138

   Purpose

   Expresses C-terminal flag-tagged human CAD R1398A A1400E R1403A R1404A L1405A in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Phage-PGK-Flag-HA-FKBP-T(G177D)

   Plasmid

   #181735

   Purpose

   for lentiviral expression of brachyury ORF in mammalian cells

   Depositor

   Charles Lin

   Article

   Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

   Insert

   T (brachyury)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   a silent mutation in the PAM site of an sgRNAt ta…

   Promoter

   Available since

   March 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

   Plasmid

   #139999

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CAG

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

   Plasmid

   #139989

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PiggyBac-TetO-Lmx1a-t2a-Pitx3-puro

    Plasmid

    #176484

    Purpose

    TetO-Lmx1a-t2a-Pitx3 in a PiggyBac vector with puromycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    Lmx1A-T2A-Pitx3 (PITX3, LMX1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid

    #140002

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV.tetO.Ascl1.U6.shRest1.U6.shRest2

    Plasmid

    #234846

    Purpose

    3rd generation lentiviral vector, expresses Ascl1 under control of TetON promoter and two shREST sequences under control of U6 promoters

    Depositor

    Malin Parmar

    Article

    Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

    Plasmid

    #140004

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FRB_T2098L-VAMP3-mCherry

    Plasmid

    #92429

    Purpose

    Expression of VAMP3 N-terminally conjugated to FRB and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    VAMP3 (Vamp3 Mouse)
    

    Use

    Tags

    FKBP rapamycin binding (FRB) domain of mTOR with …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. phH1-GBX2-gRNA

    Plasmid

    #192510

    Purpose

    Contains gRNA for GBX2

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    GBX2 (GBX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. phU6-CDX4-gRNA

    Plasmid

    #192511

    Purpose

    Contains gRNA for CDX4

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    CDX4 (CDX4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmU6-CDX1-gRNA

    Plasmid

    #192512

    Purpose

    Contains gRNA for CDX1

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    CDX1 (CDX1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ph7SK-CDX2-gRNA

    Plasmid

    #192513

    Purpose

    Contains gRNA for CDX2

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    CDX2 (CDX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

    Plasmid

    #135814

    Purpose

    Fluorescent reporter for knock-in of TdTomato into the TH locus

    Depositor

    Lee Rubin, Tim Ahfeldt

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
    

    Use

    Tags

    p2a-TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid

    #140003

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PiggyBac-TetO-En1-t2a-Foxa2-hygro

    Plasmid

    #176483

    Purpose

    TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    En1-t2a-FoxA2 (FOXA2, En1 Mouse, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid

    #139997

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

    Plasmid

    #169128

    Purpose

    AAV plasmid containing a mKate2 and a truncated PDE4D3

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Has Service

    AAV1

    Insert

    mKate2-PDE4DCatL (PDE4D Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    truncated, Catalatic domain only

    Promoter

    EF1a

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid

    #139996

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lenti.pFOXA2.GFP

    Plasmid

    #59404

    Purpose

    expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

    Depositor

    Kai-Christian Sonntag

    Article

    Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

    Insert

    400 bp FOXA2 promoter, eGFP (FOXA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pFOXA2

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lenti.pFOXA2.5'.GFP

    Plasmid

    #59407

    Purpose

    expresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

    Depositor

    Kai-Christian Sonntag

    Article

    Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

    Insert

    approx. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FOXA2

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-pGK-nAChRb2(E61C)-IRES-GFP

    Plasmid

    #164777

    Purpose

    cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

    Depositor

    Alexandre Mourot

    Article

    Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

    Insert

    nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E61C

    Promoter

    pGK

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIRES2-CMV-nAChRb2(E61C)-IRES-GFP

    Plasmid

    #164779

    Purpose

    cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

    Depositor

    Alexandre Mourot

    Article

    Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

    Insert

    nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E61C

    Promoter

    CMV

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMV2 AAV-RapaInducible-NFZ-HGF

    Plasmid

    #188749

    Purpose

    Rapamycin inducible AAV vector expressing HGF

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    HGF (HGF Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Rapamycin inducible

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-4gRNA-GBX2-RFP

    Plasmid

    #192288

    Purpose

    Expresses RFP and four sgRNAs against GBX2

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    RFP and four sgRNA GBX2 (GBX2 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge