We narrowed to 6,078 results for: nls

1. pDY0738 pCAG-NLS-SscINT

   Plasmid

   #179113

   Purpose

   SscINT expression vector

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCAG-NLS-SscINT
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDY0739 pCAG-NLS-SacINT

   Plasmid

   #179114

   Purpose

   SacINT expression vector

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCAG-NLS-SacINT
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C1 RPEL1-EGFP-3XNLS

   Plasmid

   #58469

   Purpose

   Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

   Inserts

   MRTF-A RPEL1 (Mrtfa Mouse, Synthetic)
   3X NLS with 3X stop codons
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 765 pME nls mCerulean

   Plasmid

   #195958

   Purpose

   Gateway pME fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mCerulean nuclear
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CSII-prEF1a-E2-Crimson-NLS

   Plasmid

   #125263

   Purpose

   Fluorescent reporter for cell size

   Depositor

   Jan Skotheim

   Article

   Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

   Insert

   E2-Crimson-NLS
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-WT SpCas9-NLS-6xHis

   Plasmid

   #207373

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. UbC NLS-HA-MCP-YFP

   Plasmid

   #31230

   Depositor

   Robert Singer

   Article

   Grunwald et al Nature. 2010 Sep 30. 467(7315):604-7.

   Insert

   NLS-HA-MCP-YFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-T7-NlaCas3-NLS-6xHis

   Plasmid

   #180214

   Purpose

   Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   NlaCas3c
   

   Use

   Tags

   6xHis and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA-3xFLAG-NLS-TPP1

   Plasmid

   #53585

   Purpose

   Mammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.

   Depositor

   Steven Artandi

   Article

   Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

   Insert

   TPP1 (ACD Human)
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Silent mutation to eliminate PacI site

   Promoter

   CMV

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Lp-BFPnls-LP-Cherry-T2A-NTR2.0

    Plasmid

    #219785

    Purpose

    Used for generating a transgenic line for inducing NTR2.0 expression in Cre positive cells after conversion

    Depositor

    Prayag Murawala

    Article

    García-García et al bioRxiv 2025.03.30.645595; doi: 10.1101/

    Insert

    mTagBFP2
    

    Use

    Tags

    3x SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. A3Ai-Cas9n-UGI-NLS

    Plasmid

    #109425

    Purpose

    Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-NLS-PhoCl-mCherry

    Plasmid

    #87691

    Purpose

    Express NLS-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NLS-PhoCl-mCherry
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-sACt-mCherry-NLS

    Plasmid

    #138214

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NLS (Adcy10 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS887_pTetR-P2A-BFPnls/sgAlpha

    Plasmid

    #108651

    Purpose

    Alpha satellite repeats targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Alpha satellite repeats targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-eGFP-NLS-RNF168

    Plasmid

    #133977

    Purpose

    mammalian expression vector of eGFP tagged wild type RNF168

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pGTag-NLS-TagRFP-SV40

    Plasmid

    #117805

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-FNLSHiFiNG-P2A-Puro

    Plasmid

    #136903

    Purpose

    Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFiNG
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUW-TetO-tdTomato-cNLS

    Plasmid

    #138532

    Purpose

    Lentiviral inducible expression of tdTomato with nuclear localization signal

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    tdTomato
    

    Use

    Lentiviral

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-CMV

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-Octo_VirD2_NLS-Cas9-6xHis

    Plasmid

    #232296

    Purpose

    Expresses Cas9 protein fused with octopine-type VirD2-originating NLS

    Depositor

    Hee-Sik Kim

    Article

    Le et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

    Insert

    Octo_VirD2_NLS-Cas9-6xHis
    

    Use

    CRISPR

    Tags

    6xHis and Octopine-type VirD2-derived NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-xFNLS-P2A-Puro

    Plasmid

    #110872

    Purpose

    Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xFNLS(3.7)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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