We narrowed to 6,177 results for: nls

1. TwinStrep-SUMO-NLS-BhCas12b_v4

   Plasmid

   #158615

   Purpose

   For protein expression of BhCas12b_v4

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   NLS-BhCas12b_v4
   

   Tags

   6xHIS-TwinStrep-SUMO

   Expression

   Bacterial

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDonor-tBFP-NLS-Neo

   Plasmid

   #80766

   Purpose

   Customizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   TagBFP2-3×NLS

   Expression

   Mammalian

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-REVERBA-mScarlet-NLS-PEST

   Plasmid

   #190658

   Purpose

   Fluorescent reporter for circadian rhythms

   Depositor

   Mary Teruel

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

   Insert

   Reverba-mScarlet (Nr1d1 Mouse)
   

   Expression

   Mammalian

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS613_pTetR-P2A-BFPnls/sgTelo

   Plasmid

   #108649

   Purpose

   Telomere-targeting Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Telomere-targeting sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Promoter

   U6 and hPGK

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-S5E6-dTom-nlsdTom

   Plasmid

   #135641

   Purpose

   AAV vector to drive the expression of dTomato in VIP cortical interneurons under the control of the E6 regulatory element

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Has Service

   AAV1

   Insert

   dTom-nlsdTom
   

   Use

   AAV

   Mutation

   None

   Available Since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-CMV-TO-EGFRvIII-IRES-nlsChe

   Plasmid

   #116039

   Purpose

   Supporting plasmid for testing protease circuits

   Depositor

   Michael Elowitz

   Article

   Gao et al Science. 2018 Sep 21;361(6408):1252-1258

   Insert

   EGFRvIII-IRES-nlsChe
   

   Expression

   Mammalian

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG1242 NLS-mCherry-FIREtag

   Plasmid

   #205170

   Purpose

   Expresses NLS-mCherry-FIREtag in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   NLS-mCherry-FIREtag
   

   Expression

   Mammalian

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAGGS-EGFP-NLS-3XFLAG-V5

   Plasmid

   #196090

   Purpose

   Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   GFP
   

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Promoter

   CAGGs

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. FokI-mCherry-LacI-NLS

   Plasmid

   #215096

   Purpose

   Expresses FokI-mCherry-LacI-NLS in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   FokI-mCherry-LacI-NLS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA24N-ligase-NLS-cTF

    Plasmid

    #87746

    Purpose

    IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis and NLS linker - cTF (chimeric transcriptio…

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-MBP-NLS-Geo_st

    Plasmid

    #87703

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAGGS-MCS-NLS-3XFLAG-V5

    Plasmid

    #196383

    Purpose

    Empty backbone vector to insert cDNA for strong expression in mammalian cells

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Type

    Empty backbone

    Tags

    3xFLAG and V5

    Expression

    Bacterial and Mammalian

    Promoter

    CAGGs

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)

    Plasmid

    #211861

    Purpose

    Nuclear-localized E3-DART ligase in domestication vector.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    3xNLS-HA

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AID∆C-Cas9n-UGI-NLS

    Plasmid

    #198890

    Purpose

    Express AID(1-188) BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Actication induced deaminase (AICDA Human)
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 189-198

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHsp83-NLS-HA-2xPCP-2xGFP

    Plasmid

    #71243

    Purpose

    Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    2xPCP-2xGFP
    

    Use

    P element-mediated germline transformation

    Tags

    EGFP

    Expression

    Insect

    Promoter

    Hsp83

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLR5-CAG-NLS-SNAP

    Plasmid

    #174879

    Purpose

    PiggyBac transposon vector for expression of NLS-SNAP

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    NLS-SNAP
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-FNLSNG-P2A-Puro

    Plasmid

    #136900

    Purpose

    Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSNG
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Mutation

    L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

    Promoter

    EF1s

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-mCherry-CRY2-NLS

    Plasmid

    #145259

    Purpose

    Mammalian Expression of Nuclear Control OptoConstruct with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-RAB-EKARev-NLS

    Plasmid

    #118476

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev-NLS
    

    Tags

    6xHIS, Nuclear localization signal (NLS), T7 tag …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-FKBP-ICUE3-NLS

    Plasmid

    #138255

    Purpose

    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

    Depositor

    Jin Zhang

    Article

    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

    Insert

    FKBP-ICUE3-NLS
    

    Tags

    FKBP, Nuclear localization signal (NLS), cpVenus[…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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