We narrowed to 10,009 results for: Gnas
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Plasmid#213193PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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pEX-CFP-STIM1-deltaK
Plasmid#18860DepositorInsertSTIM1 (STIM1 Human)
TagsCFPExpressionMammalianMutationStop codon at amino acid position 671 of STIM1. C…Available SinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
ADRA2A-DuET
Plasmid#213177PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA,3Q)-NLS
Plasmid#192445PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.DepositorInsertmCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hH4-AVITEV
Plasmid#74051Purposeretroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman Histone-H4 (H4C16 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianPromoterpMSCV-LTRsAvailable SinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
EBL1-bio
Plasmid#47792PurposeExpresses enzymatically monobiotinylated full-length EBL1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised EBL1
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)
Plasmid#75412PurposeModule for the expression of the Renilla Luciferase with the silencing suppressor P19DepositorInsertRenilla / P19
UseLuciferase and Synthetic BiologyExpressionPlantPromoter35SAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
mApple-proα2(I)
Plasmid#119840PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with mApple between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
TagsmAppleExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-CXCR4-VN
Plasmid#98964PurposeFLAG-tagged human CXCR4 fused to N-terminus of split VenusDepositorInsertCXCR4 (CXCR4 Human)
TagsFLAG (dykdddd) epitope tag, Signal/leader sequenc…ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-CXCR4-VC
Plasmid#98967Purposemyc-tagged human CXCR4 fused to C-terminus of split VenusDepositorInsertCXCR4 (CXCR4 Human)
TagsSignal/leader sequence from Influenza A virus HA …ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D)
Plasmid#20174DepositorInsertpEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D) (STIM2 Human)
TagsYFPExpressionMammalianMutation3 mutations (K83A,D84N,G86D) in the EF hand of ST…Available SinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
mVenus-proα2(I)
Plasmid#119838PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with mVenus between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
TagsmVenusExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc1/bC - AVITEV
Plasmid#74059Purposeretroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc1, isoform beta-C (NFATC1 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianPromoterpMSCV-LTRsAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF
Plasmid#79889PurposeDonor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-proα2(I)
Plasmid#119836PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
TagsmCeruleanExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT
Plasmid#89890PurposeBAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.DepositorInsertHA-BirA-2A-citrine
UseUnspecifiedTagsCitrineAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRalpha-Y206S
Plasmid#136435PurposeMammalian expression plasmid for c-myc-tagged PDGFRA (Y206S mutant)DepositorInsertPDGFRalpha (PDGFRA Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationY206S (disrupts PDGF binding, but still binds HCM…PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-bio-myc-mCE 2-210
Plasmid#112715PurposeExpresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cellsDepositorInsertRngtt (Rngtt Mouse)
Tagsbiotinylation signal peptide (Tagwerker et al. 20…ExpressionMammalianMutationSilent mutation at Arg 132 (CGT to CGC), deleted …PromoterCMVAvailable SinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
TLP-bio
Plasmid#47787PurposeExpresses enzymatically monobiotinylated full-length TLP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised TLP
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_1431400-bio
Plasmid#47791PurposeExpresses enzymatically monobiotinylated full-length PF3D7_1431400 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised PF3D7_1431400
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only