We narrowed to 13,645 results for: sequence
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Plasmid#117271PurposeExpresses Nfib and hygromycin resistance gene under control of tetO sequence.DepositorInsertNfib (Nfib Mouse)
UseLentiviralTagsIRES and hygromycin resistant cassette (C termina…ExpressionMammalianPromotertet-onAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
tetO.Sox9.Puro
Plasmid#117269PurposeExpresses Sox9 and puromycin resistance gene under control of tetO sequence.DepositorInsertSox9 (Sox9 Mouse)
UseLentiviralTagsT2A and puromycin resistant cassette (C terminal …ExpressionMammalianPromotertet-onAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124428PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
ExpressionMammalianAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
ODD-Luciferase-pcDNA3
Plasmid#18965DepositorInsertODD-Firefly Luciferase (HIF1A Human)
ExpressionMammalianMutationHIF1a ODD sequence fused in frame to luciferase O…Available SinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
CMV-NLS-R-GECO
Plasmid#32462PurposeMammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorInsertR-GECO1.0
TagsNLS sequence DPKKKRKVExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-RiboL1-jGCaMP8f
Plasmid#169249PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.DepositorInsertRiboL1-jGCaMP8f
UseAAVTags6xHisExpressionMammalianPromoterhSynAvailable SinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST pcDNA5-FRT/TO-eGFP-Rif1
Plasmid#52506Purposemammalian expression vector of Rif1 siRNA resistantDepositorAvailable SinceJune 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-hSyn-RiboL1-jGCaMP8s
Plasmid#169247PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.DepositorInsertRiboL1-jGCaMP8s
UseAAVTags6xHisExpressionMammalianPromoterhSynAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-MCART1
Plasmid#133247PurposeExpress FLAG-tagged MCART1 in mammalian cellsDepositorInsertMCART1 (SLC25A51 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationcodon-optimized for expression in human cells and…Available SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pk7-GR-GFP
Plasmid#15534DepositorInsertGlucocorticoid receptor (NR3C1 Human)
TagsGFPExpressionMammalianMutationThe glucocorticoid receptor was cloned into the X…Available SinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry
Plasmid#124429PurposeExpresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(TEV cleavage seq) T2A mCherry
ExpressionMammalianAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
mClY-N1
Plasmid#90457PurposeA YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.DepositorInsertmClY-N1
ExpressionMammalianMutationEYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…Available SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
Plasmid#119946PurposeExpresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
Tags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianPromoterTetCMVAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1
Plasmid#92120PurposeExpression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.DepositorAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.cTNT.iCre
Plasmid#69916PurposeUsed to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.DepositorHas ServiceAAV9Insertimproved cre (iCre) recombinase and tdTomato
UseAAVTagsThe iCre coding frame and tdTomato coding frame i…PromoterChicken cardiac troponin TAvailable SinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
PG13-luc (wt p53 binding sites)
Plasmid#16442PurposeLuciferase reporter containing 13 copies of the p53-binding consensus sequenceDepositorAvailable SinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pU6-ACTA2_gRNA1-SpCas9-T2A-GFP
Plasmid#126706Purposeto express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequenceDepositorInsertACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
UseCRISPRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
plentiCMV10x-sct-pp65-b2m-HLA-A0201
Plasmid#196513PurposeExpress single-chain pMHC with sequencing tag for 10x genomicsDepositorInsertsingle chain of a pp65 peptide, b2m and HLA A0201
UseLentiviralTagsNAExpressionMammalianMutationY84C, 10xgenomics compatible tagPromoterCMVAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
BMR1_03g02090-bio-His
Plasmid#107674PurposeExpresses enzymatically monobiotinylated full-length BMR1_03g02090 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertBMR1_03g02090
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-nosT
Plasmid#71272PurposeEntry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertnopaline synthase terminator
UseGatewayAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only