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HRE-luciferase

Plasmid

#26731

Purpose

Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

Depositor

Navdeep Chandel

Publication

Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

Insert

Hypoxia Responsive Elements (3)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

90 Articles

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pGP-AAV-syn-jGCaMP8m-WPRE

Plasmid

#162375

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Publication

Zhang et al Janelia Research Campus, Online Resource

Has service

AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

Synapsin

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pmScarlet_alphaTubulin_C1

Plasmid

#85045

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pZac2.1 gfaABC1D-tdTomato

Plasmid

#44332

Depositor

Baljit Khakh

Publication

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Has service

AAV5

Insert

tdTomato

Use

AAV; Adeno-associated virus ()

Expression

Mammalian

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

36 Articles

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pAAV-EF1a-FLEX-GTB

Plasmid

#26197

Depositor

Edward Callaway

Publication

Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

Insert

Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

TVA, rabies glycoprotein are mammalian codon opti…

Available since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pShuttle-Cre-HA

Plasmid

#16583

Depositor

Bert Vogelstein

Publication

Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

Insert

cre recombinase (cre P1 bacteriophage)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-PE6b

Plasmid

#207852

Purpose

Mammalian expression of PE6b prime editor

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6b

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

Plasmid

#141383

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

13 Articles

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pAC94-pmax-dCas9VP160-2A-puro

Plasmid

#48226

Purpose

dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro

Use

CRISPR

Tags

2A-puro, HA Tag, and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pEGFP-N1-ACTR3

Plasmid

#8462

Depositor

Matthew Welch

Publication

Welch et al J Cell Biol 1997 Jul 28;138(2):375-84.

Insert

ACTR3 (ACTR3 Human)

Tags

GFP

Expression

Mammalian

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCSII-EF/mt-(n1)StayGold

Plasmid

#185823

Purpose

A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

Depositor

Atsushi Miyawaki

Publication

Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

Insert

mt-(n1)StayGold

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MSP1830

Plasmid

#70708

Purpose

Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

Depositor

Keith Joung

Publication

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Insert

mammalian codon-optimized KKH variant Staphylococcus aureus Cas9

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Mammalian

Mutation

E782K, N968K and R1015H in SaCas9

Promoter

CAG

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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GFP-RB FL

Plasmid

#16004

Depositor

Olimpia Meucci

Publication

Khan et al Cell Death Differ. 2008 Oct . 15(10):166

Insert

Rb (RB1 Human)

Tags

EGFP

Expression

Mammalian

Available since

June 19, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pBabe puro HA PIK3CA H1047R

Plasmid

#12524

Purpose

Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

Depositor

Jean Zhao

Publication

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H1047R. Tumor-derived allele.

Available since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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5HT6-mCherry-G-GECO1.0

Plasmid

#47500

Purpose

Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

Depositor

Takanari Inoue

Publication

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

G-GECO1.0

Tags

5HT6 and mCherry

Expression

Mammalian

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

Plasmid

#176271

Purpose

Expression of CasMINI-V3.1 in mammalian cells.

Depositor

Stanley Qi

Publication

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI-V3.1

Expression

Mammalian

Promoter

CMV

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG-YAP1

Plasmid

#66853

Purpose

Express FLAG-tagged YAP1 in mammalian cells

Depositor

Yutaka Hata

Publication

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

YAP1 (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pICH86966::AtU6p::sgRNA_PDS

Plasmid

#46966

Purpose

Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

Depositor

Sophien Kamoun

Publication

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p::sgRNA_PDS

Use

CRISPR; Plant expression

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pRK5-HA GST RagB 99L

Plasmid

#19303

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Tags

GST and HA

Expression

Mammalian

Mutation

Q99L

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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