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We narrowed to 22,868 results for: REP

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Showing: 961 - 980 of 22868 results

  10. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100900-plasmid-map-sequence-id-457690

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  14. 5'UTR CGG 99x FMR1-EGFP

    Plasmid
    #63091
    Purpose
    Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63091-plasmid-map-sequence-id-459961

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  18. pUbiGUSPlus

    Plasmid
    #64402
    Purpose
    Ubiquitin promoter driving synthetic optimised GUSPlus gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.
    Depositor
    Claudia Vickers
    Article
    Vickers et al Plant Cell Rep. 2003 Sep;22(2):135-40. E
    Insert
    ubiquitin (Ubi-1) promoter
    Tags
    GUSPlus reporter
    Expression
    Plant
    Promoter
    Ubi-1
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64402-plasmid-map-sequence-id-108183

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  20. pDAC565

    Plasmid
    #195242
    Purpose
    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    Expression
    Mammalian
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195242-plasmid-map-sequence-id-382950

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Showing: 961 - 980 of 22868 results