161,186 results

1. pCDH_blast_MCS_Nard_GFP_LAMIN

   Plasmid

   #167340

   Purpose

   Expresses GFP fused to lamin A (LA) in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CaMKIIa-hsChRmine-oScarlet-KV 2.1-WPRE

   Plasmid

   #183521

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

   Insert

   hsChRmine-oScarlet-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   H33R

   Promoter

   CaMKIIa

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Xla.Tubb2:hArr-TEV;he1.1:eYFP

   Plasmid

   #218859

   Purpose

   Xla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hArr
   

   Use

   Tags

   human Arrestin-Tobbaco Etch Virus protease fusion…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. BBa_J72114-BBa_J72104

   Plasmid

   #40783

   Depositor

   Christopher Anderson

   Article

   Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

   Insert

   RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ
   

   Use

   Luciferase and Synthetic Biology; Phagemid

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUK21

   Plasmid

   #49788

   Purpose

   E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1991 Apr;100:189-94.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lacZ

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-TetOne-Puro-p21

   Plasmid

   #171122

   Purpose

   Doxycycline inducible expression of p21

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   p21 (CDKN1A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKRG3-mU6-PUb-hSpCas9

   Plasmid

   #162162

   Purpose

   Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   N/A

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBR15.2-2H12.D11opt-scarRef

   Plasmid

   #221150

   Purpose

   cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen2.1
   mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   Constitutive and None (same as cdGreen2.1)

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET His6 LIC cloning vector (2Bc-T)

   Plasmid

   #37236

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid

    #154840

    Purpose

    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    HYGR∆ encodes aa 1-226

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTN14

    Plasmid

    #178945

    Purpose

    Expresses AtTIR1(F79G) in C elegans

    Depositor

    Masato Kanemaki

    Article

    Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

    Insert

    AtTIR1(F74G)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIP-Flag-TfebAA

    Plasmid

    #79014

    Purpose

    For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Tfeb (Tfeb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S142A, S211A

    Promoter

    pCAGGs

    Available since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCyRFP1-Cdc42

    Plasmid

    #84360

    Purpose

    fusion protein of Cdc42, FRET donor

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCyRFP1-Cdc42
    

    Use

    Tags

    mCyRFP1 N terminal fusion to Cdc42

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOpen-K12polLF (exo-)

    Plasmid

    #165522

    Purpose

    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. wtBxb1

    Plasmid

    #222337

    Purpose

    Plasmid for expressing wild type Bxb1 in mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized wild type Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Paxillin-pEGFP

    Plasmid

    #15233

    Depositor

    Rick Horwitz

    Article

    Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

    Insert

    paxillin (PXN Chicken)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV GFP

    Plasmid

    #49055

    Purpose

    adeno-associated virus (AAV) encoding a green fluorescent protein

    Depositor

    Fred Gage

    Article

    Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF0495

    Plasmid

    #143031

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EGR2 (EGR2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET_His6-SUMO-TEV-TtCsm6

    Plasmid

    #172486

    Purpose

    Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
    

    Use

    Tags

    His-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and lac operator

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-TA-ERP2

    Plasmid

    #80477

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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