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pLRD15

Plasmid

#208267

Purpose

Production of ent-sandaracopimaradiene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL10

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Hygro

Plasmid

#186671

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Hygromycin (YTHDF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Puro

Plasmid

#186670

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Puromycin (YTHDF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-R2L3-IRESpacDeltaTKpA

Plasmid

#139535

Purpose

Provides IRES-pacDeltaTK-pA cassette on Gateway entry vector

Depositor

Minoru Ko

Insert

PacTK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-MMACHC [N230/21R]

Plasmid

#114555

Purpose

Mammalian Expression Plasmid of anti-MMACHC (Human). Derived from hybridoma N230/21.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-MMACHC (Homo sapiens) recombinant mouse monoclonal antibody (MMACHC Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Otx1

Plasmid

#84315

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Otx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Otx1 (Otx1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(457-924)

Plasmid

#42006

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa457-924

Promoter

CMV

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(1-456)

Plasmid

#42005

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa1-456

Promoter

CMV

Available since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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pCDHblast MCSNard OST-LMNA

Plasmid

#22661

Depositor

Tom Misteli

Article

Pegoraro et al Nat Cell Biol. 2009 Oct . 11(10):1261-7.

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

Onestrep Tag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC26A1

Plasmid

#131972

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC26A1 (SLC26A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1-PKM2

Plasmid

#64698

Purpose

Expression of GFP tagged PKM2 in mammalian cells

Depositor

Axel Ullrich

Article

Stetak et al Cancer Res. 2007 Feb 15;67(4):1602-8.

Insert

PKM2 (PKM Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pTC-ApoE-Tet

Plasmid

#85578

Purpose

Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liver

Depositor

Ursula Ehmer

Article

Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

Insert

CreER

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

ApoE.HCR.hAAT

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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W45A mutant of H3K9me3 biosensor

Plasmid

#120808

Purpose

FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3

Use

Tags

Expression

Mammalian

Mutation

Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

Promoter

CMV

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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lentiTet-P2A-mCherry BlastR

Plasmid

#186743

Purpose

Dox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

P2A-mcherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hM4D(Gi)-mCherry

Plasmid

#50477

Purpose

Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter

Depositor

Bryan Roth

Has Service

AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

hM4D(Gi)-mCherry (CHRM4 Human)

Use

AAV

Tags

mCherry

Expression

Mutation

See supplemental documents for DREADD mutations

Promoter

CaMKIIa

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-GFP

Plasmid

#83375

Purpose

Expression of Myc tagged GFP

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

Myc-GFP

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-sfCherry(1-10)

Plasmid

#70222

Purpose

Expresses sfCherry(1-10) in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hADARB2-3xFLAG

Plasmid

#165470

Purpose

Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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V96 ELP

Plasmid

#68392

Purpose

Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V96 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408)

Plasmid

#191728

Purpose

AI plasmid ALIAS: AiP12408. Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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