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Showing: 9881 - 9900 of 65831 results

  5. pTransposon.CAG.CasRX-P2A-Blast

    Plasmid
    #212965
    Purpose
    Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.
    Depositor
    Roland Rad
    Publication
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CasRx
    Use
    Transposon
    Tags
    P2A-Blasticidin resistance
    Expression
    Mammalian
    Promoter
    CAGGS
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212965-plasmid-map-sequence-id-422557

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  6. pAAV-SynaptoTAG2

    Plasmid
    #175275
    Purpose
    AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.
    Depositor
    Wei Xu
    Publication
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    EGFP-Synaptobrevin-2; tdTomato (Vamp2 )
    Use
    AAV
    Tags
    EGFP and tdTomato (P2A cleavage)
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    175275-plasmid-map-sequence-id-349084

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  7. lentiCRISPR - EGFP sgRNA 4

    Plasmid
    #51763
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 4
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    51763-plasmid-map-sequence-id-79382

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  8. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid
    #187963
    Purpose
    mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    osTIR1
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker and T2A
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187963-plasmid-map-sequence-id-373709

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  10. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v2 (B.1.617.1 v2)

    Plasmid
    #212429
    Purpose
    Encodes SARS-CoV-2 spike T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E484Q,D614G,P681R,Q1071H for pseudovirus production
    Depositor
    Alejandro Balazs
    Publication
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E484Q,D614G,P681R,Q1071H (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E…
    Promoter
    CAG
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    212429-plasmid-map-sequence-id-432769

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  11. pILGFPEasy9R

    Plasmid
    #218291
    Purpose
    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.
    Depositor
    Claudia Vickers
    Publication
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    Expression
    Yeast
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    218291-plasmid-map-sequence-id-433736

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  13. dCas9-CBP PHD-HAT

    Plasmid
    #179548
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179548-plasmid-map-sequence-id-354746

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  14. dCas9-p300 RING-PHD-HAT

    Plasmid
    #179546
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179546-plasmid-map-sequence-id-354744

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  15. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid
    #61361
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61361-plasmid-map-sequence-id-99794

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  16. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  17. JO-pH720-SARS-CoV-2-S-tail-Cys1254Ala-Thr1273Glu

    Plasmid
    #218454
    Purpose
    Bacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike (with Cys1254Ala and Thr1273Glu substitutions) and an N-terminal eXact tag
    Depositor
    Syed Saif Hasan
    Publication
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag and Cys1254Ala and Thr1273Glu substitutions (S SARS-CoV-2)
    Tags
    eXact
    Expression
    Bacterial
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218454-plasmid-map-sequence-id-436194

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  19. pcDNA3.1-ERBB3-V5/HIS

    Plasmid
    #201104
    Purpose
    expression of human ERBB3 receptor tyrosine kinase in mammalian cells
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human ERBB3 receptor tyrosine kinase, full length, wildtype (ERBB3 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201104-plasmid-map-sequence-id-394500

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Showing: 9881 - 9900 of 65831 results