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pBV-Luc/Del-6

Plasmid

#14969

Depositor

Joan Massague

Publication

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/+334) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-109/+334 (relative to the P2 transcription initi…

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCEP4_WT_OCT4

Plasmid

#40629

Depositor

James Thomson

Publication

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

POU5F1 (POU5F1 Human)

Expression

Mammalian

Promoter

EF1-alpha

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pEYFP-Synectin

Plasmid

#35791

Depositor

Arie Horowitz

Publication

Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Synectin (Gipc1 Mouse)

Tags

EYFP

Expression

Mammalian

Mutation

K111R

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLVE-hTGFB3-IRES-Red

Plasmid

#52580

Purpose

2nd generation lentiviral transfer vector driving expression of human TGF-beta3 and RFP in mammalian cells

Depositor

Charles Gersbach

Publication

Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

human transforming growth factor-beta3 (TGFB3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1alpha

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPRIME-CMV-GFP-recipient

Plasmid

#11657

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling GFP expression

Depositor

Stephen Elledge

Publication

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDG1-PHP.eB

Plasmid

#220799

Purpose

AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

Depositor

Sam Golden

Publication

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

E4, E2A, VA, AAV2 rep and PHP.eB cap genes

Use

AAV; Aav helper vector

Expression

Mammalian

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1D V5-His-TOPO-KGA.wt

Plasmid

#110332

Purpose

Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Publication

Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

KGA glutaminase (GLS Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pINDUCER12 (miR-RUL)

Plasmid

#46935

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Publication

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA3 Flag MKK7a1 (ala)

Plasmid

#14539

Depositor

Roger Davis

Publication

Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MKK7a1(Ala) (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Ser-198Ala Thr-202Ala

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pT7goldRNA

Plasmid

#47930

Purpose

In vitro transcription of golden gRNA

Depositor

Wenbiao Chen

Publication

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Insert

golden gRNA (slc24a5 Zebrafish)

Use

CRISPR

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-D1cpV

Plasmid

#37479

Depositor

Amy Palmer, Roger Tsien

Publication

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

D1cpV second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pUSE-Src-YF-UniRapR-mCerulean-myc

Plasmid

#45381

Purpose

Mammalian expression of constitutively active Src kinase (YF mutant) fused to uniRapR, which switches on activity in the presence of rapamycin. mCerulean for fluorescence visualization.

Depositor

Klaus Hahn

Publication

Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Src-YF-UniRapR-mCerulean-myc

Tags

Myc, Myr, and mCerulean

Expression

Mammalian

Mutation

changed mSrc Tyrosine 529 to Phenylalanine

Promoter

CMV

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Orange Nano-lantern/pcDNA3

Plasmid

#67661

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Orange Nano-lantern

Expression

Mammalian

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGF-mSox2-ONL

Plasmid

#65719

Purpose

mSox2 reporter plasmid (expresses ONL)

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

murine Sox2 promoter and ONL

Use

Lentiviral and Luciferase

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pSEVA331Bb

Plasmid

#78269

Purpose

pSEVA331 with Biobrick multiple cloning site

Depositor

Tom Ellis

Publication

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Type

Empty backbone

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry-CAD

Plasmid

#73566

Purpose

Soluble red fluorescent activator of Orai1 channels

Depositor

Michael Cahalan

Publication

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry-CAD (STIM1 342-448) (STIM1 Synthetic, Human)

Tags

6x His and mCherry

Expression

Mammalian

Promoter

CMV Immediate Early

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBabe DN-FOXO1-HA neo

Plasmid

#45814

Depositor

Kevin Janes

Publication

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

FOXO1 (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

aa1-255

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLKO-1 shRev-Erbalpha

Plasmid

#22747

Depositor

Bruce Spiegelman

Publication

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

shRev-Erbalpha (Nr1d1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET24a-VHH-APEX2

Plasmid

#109420

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Publication

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope

Tags

BAP, HA, His6, and T7

Expression

Bacterial

Promoter

T7

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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IL6R PLAK1

Plasmid

#161518

Purpose

Expresses IL6 receptor (full length) in mammalian cells.

Depositor

Yoav Shaul

Publication

Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

Insert

Interleukin 6 Receptor (IL6R Human)

Use

Lentiviral

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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