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Showing: 121 - 150 of 309 results

  1. pACT5FVMF(f)

    Plasmid
    #110549
    Purpose
    Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008
    Depositor
    David Russell
    Article
    Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10
    Insert
    EGFP, FV LTRs
    Use
    Qpcr control for titering foamy virus
    Tags
    Expression
    Mutation
    Promoter
    promoter: MLV LTR
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110549-plasmid-map-sequence-id-212295

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  5. pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

    Plasmid
    #214265
    Purpose
    Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.
    Depositor
    D. James Surmeier
    Insert
    Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
    Use
    AAV
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Synapsin
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214265-plasmid-map-sequence-id-429396

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  12. pPT-PCR C2

    Plasmid
    #141247
    Purpose
    PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Algal Research, 2020. 50, 102014
    Insert
    Mitochondrial Genome of Phaeodactylum tricornutum
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Repeat region removed, additional minor mutations…
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    141247-plasmid-map-sequence-id-377468

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  16. pPT-TAR C1

    Plasmid
    #141246
    Purpose
    Transformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Algal Research, 2020. 50, 102014
    Insert
    Mitochondrial Genome of Phaeodactylum tricornutum
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    141246-plasmid-map-sequence-id-367010

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  17. pPT-PCR C1

    Plasmid
    #141248
    Purpose
    PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Algal Research, 2020. 50, 102014
    Insert
    Reduced Mitochondrial Genome of Phaeodactylum tricornutum
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Repeat region removed, additional minor mutations…
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    141248-plasmid-map-sequence-id-389038

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  19. pJRoC30_VP8H

    Plasmid
    #188062
    Purpose
    PROSS-designed versatile peroxidase 8H
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35
    Insert
    PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
    Use
    Tags
    S. cerevisiae native α-factor prepro-leader signa…
    Expression
    Yeast
    Mutation
    38 PROSS mutations, described in publication
    Promoter
    Available since
    Aug. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    188062-plasmid-map-sequence-id-370147

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  21. dRT-pMDLg/pRRE

    Plasmid
    #60488
    Purpose
    gag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)
    Depositor
    Boris Fehse
    Article
    Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103
    Insert
    HIV-1 gag, HIV-1 pol with inactive reverse transcriptase
    Use
    Tags
    Expression
    Mutation
    changed Asp at position 249 + 250 to Val
    Promoter
    Available since
    Oct. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    60488-plasmid-map-sequence-id-96845

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  23. pUB-Cas9

    Plasmid
    #86556
    Purpose
    For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)
    Depositor
    Andreas Weber
    Article
    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Cas9 is codon optimized for C. reinhardtii
    Promoter
    UBIQUITIN10
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86556-plasmid-map-sequence-id-176429

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  24. pdUA-lacIN

    Plasmid
    #141079
    Purpose
    Plasmid vector for testing DNA binding proteins by the I-Block assay.
    Depositor
    Antal Kiss
    Article
    Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28
    Type
    Empty backbone
    Use
    Vector for the i-block assay designed to study dn…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Escherichia coli lacIN promoter
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    141079-plasmid-map-sequence-id-310860

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  26. pJC30X-BS(X)

    Plasmid
    #58958
    Purpose
    Encodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.
    Depositor
    Thomas Fox
    Article
    Cohen et al Mitochondrion. 2001 Aug;1(2):181-9.
    Insert
    Enhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
    Use
    Tags
    First 8 codons of COX3
    Expression
    Mutation
    GFP modified as described: CORMACK, B. P., R. H. …
    Promoter
    n/a
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. Barcode backbone

    Plasmid
    #158665
    Purpose
    This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination
    Depositor
    Ravid Straussman
    Article
    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    hcRED
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    158665-plasmid-map-sequence-id-309764

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Showing: 121 - 150 of 309 results