We narrowed to 54,294 results for: Ran

1. PS-mOrange-Paxillin-22

   Plasmid

   #57809

   Purpose

   Localization: Focal Adhesions, Excitation: 548 / 634, Emission: 565 / 662

   Depositor

   Michael Davidson

   Insert

   Paxillin
   

   Use

   Tags

   PS-mOrange

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. mOrange2-CENPB-N-22

   Plasmid

   #57946

   Purpose

   Localization: Nucleus/Centromeres, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Insert

   CENPB (CENPB Human)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   aa 1-169 of CENPB (DNA Binding Domain)

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pBactin-mOrange2-CI

   Plasmid

   #196852

   Purpose

   Expression of red fluorescent reporter mOrange driven by the strong Beta-actin promoter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EGFP-RanGAP*

   Plasmid

   #175237

   Purpose

   Bacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the Km

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   RANGAP1 (RANGAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Amino acid 562 F to A

   Promoter

   tac

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EGFP-RanGAP

   Plasmid

   #175235

   Purpose

   Bacterial expression and purification, high affinity SUMOylation substrate

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   RANGAP1 (RANGAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. dGFP-Granuphilin

   Plasmid

   #170868

   Purpose

   Mammalian expression of dGFP-Granuphilin.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   Synaptotagmin-like 5 (SYTL5 Human)
   

   Use

   Tags

   dEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHH0103_ABCE1_ABC-tran

   Plasmid

   #109853

   Purpose

   Protein expression and purification of ABCE1_ABC-tran

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   ABCE1_ABC-tran (ABCE1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mOrange-imp α

   Plasmid

   #119720

   Purpose

   Expresses mOrange-tagged human importinα in mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

   Insert

   importinα (KPNA2 Human)
   

   Use

   Tags

   mOrange

   Expression

   Mammalian

   Mutation

   contains amino acids 251-529

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDOE20 mC VN transient

   Plasmid

   #124247

   Purpose

   Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in directly-transfected plant cells.

   Depositor

   Attila Fehér

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mOrange2-Cx26-7

    Plasmid

    #57949

    Purpose

    Localization: Gap Junctions, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Cx26
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

11. mOrange2-Cx43-7

    Plasmid

    #57951

    Purpose

    Localization: Gap Junctions, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Cx43
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

12. mOrange2-LC-Myosin-N-7

    Plasmid

    #57967

    Purpose

    Localization: Light Chain Myosin, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Insert

    LC-Myosin
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. mOrange2-VASP-5

    Plasmid

    #57975

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Insert

    VASP
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. mOrange2-Cx32-7

    Plasmid

    #57950

    Purpose

    Localization: Gap Junctions, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Insert

    Cx32 (GJB1 Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

15. mOrange2-Talin-R-15

    Plasmid

    #57973

    Purpose

    Localization: Focal Adhesions, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Insert

    Talin R
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Talin Rod Domain

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. LI C-D mOrange noATG

    Plasmid

    #54441

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Insert

    mOrange without Start codon
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-Ran(Q69L)

    Plasmid

    #42048

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    Ran (RAN Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q69L

    Promoter

    Available since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mARGAna (cassette 1, transient)

    Plasmid

    #197588

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mARGAna (cassette 2, transient)

    Plasmid

    #197589

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-Tol2 transposase

    Plasmid

    #158774

    Purpose

    Expression of Tol2 transposase

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    Tol2 tranposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Tol2 codon usage frequency well suitable for muri…

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pORANGE GFP-Grin2a KI

    Plasmid

    #131486

    Purpose

    Endogenous tagging of GluN2a: N-terminal (amino acid position: A25)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Grin2a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pORANGE GFP-Grin2b KI

    Plasmid

    #131487

    Purpose

    Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Grin2b Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. T7-CMVtrans-FFLuc-polyA

    Plasmid

    #101156

    Purpose

    Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

    Depositor

    Marcel Bruchez

    Article

    Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mKusabira Orange-C1 (mKO)

    Plasmid

    #54834

    Purpose

    Localization: C1 Cloning Vector, Excitation: 548, Emission: 561

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

    Type

    Empty backbone

    Use

    Tags

    mKusabira Orange

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pQC membrane TdTomato IX

    Plasmid

    #37351

    Depositor

    Connie Cepko

    Article

    Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

    Insert

    membrane TdTomato
    

    Use

    Tags

    Palmitoylation signal*

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Banshee shRandom mCherry

    Plasmid

    #80145

    Purpose

    retroviral expression of random shRNA

    Depositor

    Ellen Rothenberg

    Insert

    random shRNA
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDae079 (eMutaT7[transition])

    Plasmid

    #187622

    Purpose

    Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Inserts

    TadA-8e-T7RNAP
    PmCDA1-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pORANGE GFP-Grin1 KI

    Plasmid

    #131485

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Grin1, Grin1 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pORANGE GFP-Camk2a KI

    Plasmid

    #131484

    Purpose

    Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Camk2a, Camk2a Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-P-RAN-GFP1

    Plasmid

    #106408

    Purpose

    P-RAN-GFP1 (RANbody GFP1::vHRP)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    P-RAN-GFP1
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pORANGE Tubb3-GFP KI

    Plasmid

    #131497

    Purpose

    Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Tubb3, Tubb3 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a-LSSmOrange-CNA35

    Plasmid

    #61605

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    LSSmOrange and CNA35
    

    Use

    Tags

    His tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pORANGE GFP-Grin1 KI #3

    Plasmid

    #139659

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pORANGE GFP-Nlgn3 KI

    Plasmid

    #131501

    Purpose

    Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Nlgn3, Nlgn3 Mouse, Rat)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Ornithine transcarbamylase (OTC)

    Plasmid

    #71877

    Purpose

    For mitochondrial unfolded protein response research

    Depositor

    Nicholas Hoogenraad

    Article

    Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

    Insert

    OTC (Otc Rat)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pORANGE GFP-Grin1 KI #2

    Plasmid

    #139658

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pORANGE Dlg4-GFP KI

    Plasmid

    #131477

    Purpose

    Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Dlg4, Dlg4 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTransposon.CAG.CasRX-P2A-Blast

    Plasmid

    #212965

    Purpose

    Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    CasRx
    

    Use

    Transposon

    Tags

    P2A-Blasticidin resistance

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHIV-BRAFV600E-mOrange2

    Plasmid

    #110732

    Purpose

    Mammalian Expression of BRAFV600E IRES H2B-mOrange2

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    BRAFV600E IRES H2B-mOrange2 (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    BRAF V600E

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pORANGE GFP-Syt7 KI

    Plasmid

    #131488

    Purpose

    Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Syt7, Syt7 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLD1 translation factors

    Plasmid

    #117760

    Purpose

    BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 1-13
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLD3 translation factors

    Plasmid

    #117762

    Purpose

    BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 22-30
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLD2 translation factors

    Plasmid

    #117761

    Purpose

    BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 14-21
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pORANGE Cplx1-GFP KI

    Plasmid

    #131475

    Purpose

    Endogenous tagging of Complexin1: C-terminal (amino acid position: L128)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cplx1, Cplx1 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pORANGE GFP-CADPS KI

    Plasmid

    #131482

    Purpose

    Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cadps Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pORANGE GFP-Actb KI

    Plasmid

    #131479

    Purpose

    Endogenous tagging of βactin: N-terminal (amino acid position: D2)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Actb, Actb Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLentiCrispr-V2-mOrange (V2mO)

    Plasmid

    #140206

    Purpose

    Lenti Crispr/Cas9 with mOrange fluorescent marker

    Depositor

    Jaffer Ajani

    Article

    Jin et al PLoS One. 2020 Feb 13;15(2):e0228910. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pELF1a

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pGEMHE-membrane-mEGFP

    Plasmid

    #105526

    Purpose

    T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    GNAI2 (GNAI2 Human)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    fragment encoding the plasma membrane targeting s…

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. pORANGE Gria1-GFP KI

    Plasmid

    #131489

    Purpose

    Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gria1, Gria1 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCS-membrane-ceruleanFP

    Plasmid

    #53749

    Purpose

    Contains membrane localized cerulean fluorescent protein in pCS expression vector.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    2x membrane linker
    

    Use

    Tags

    Cerulean

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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