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Showing: 81 - 100 of 61328 results

  2. pDual-eGFP(Stop66)

    Plasmid
    #63218
    Purpose
    Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Stop66)
    Use
    Lentiviral
    Tags
    T7 and his6
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pDual-eGFP(W66)

    Plasmid
    #63217
    Purpose
    Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(W66)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pAF161

    Plasmid
    #135926
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mCerulean3
    Use
    Transposon
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135926-plasmid-map-sequence-id-270079

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  6. pAF163

    Plasmid
    #135927
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mNeptune2 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mNeptune2
    Use
    Transposon
    Tags
    mNeptune2
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135927-plasmid-map-sequence-id-270084

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  9. pCR8-CDK12(sgR)

    Plasmid
    #208346
    Purpose
    Gateway cloning entry vector with CDK12 with sgRNA resistant silent mutations. Must be recombined into a DEST vector for expression.
    Depositor
    Peter S Nelson
    Article
    Frank et al eLife 2025
    Insert
    cyclin dependent kinase 12 (CDK12 Human)
    Use
    Gateway: entry vector
    Mutation
    Silent mutations in A88, K90, D92, R94, T715, S71…
    Available Since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    208346-plasmid-map-sequence-id-415904

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  10. pCR8-CDK12

    Plasmid
    #208347
    Purpose
    Gateway cloning entry vector with CDK12. Must be recombined into a DEST vector for expression.
    Depositor
    Peter S Nelson
    Article
    Frank et al eLife 2025
    Insert
    cyclin dependent kinase 12 (CDK12 Human)
    Use
    Gateway: entry vector
    Available Since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    208347-plasmid-map-sequence-id-416919

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  12. pLenti-sgStuffer-GFP-Puro

    Plasmid
    #208349
    Purpose
    Lentivirus sgRNA backbone with GFP and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI)
    Depositor
    Peter S Nelson
    Article
    Frank et al eLife 2025
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Promoter
    hU6
    Available Since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    208349-plasmid-map-sequence-id-416750

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  14. FUCGW-DEST

    Plasmid
    #208408
    Purpose
    Lentivirus compatible with LR Gateway cloning for UBC-driven gene expression with CMV-GFP in backbone
    Depositor
    Peter S Nelson
    Article
    Frank et al eLife 2025
    Type
    Empty backbone
    Use
    Lentiviral; Gateway: dest vector
    Promoter
    UBC
    Available Since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    208408-plasmid-map-sequence-id-416921

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  15. FUCRW-DEST

    Plasmid
    #208409
    Purpose
    Lentivirus compatible with LR Gateway cloning for UBC-driven gene expression with CMV-RFP in backbone
    Depositor
    Peter S Nelson
    Article
    Frank et al eLife 2025
    Type
    Empty backbone
    Use
    Lentiviral; Gateway: dest vector
    Promoter
    UBC
    Available Since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    208409-plasmid-map-sequence-id-416920

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Showing: 81 - 100 of 61328 results