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Plasmid#112008PurposeFor cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3DepositorInsertaxon-GCaMP6s-P2A-mRuby3
UseAAV and Cre/LoxTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSynapsin1Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNR4A1-donor
Plasmid#112295PurposeCRISPR donor plasmid to tag human transcription factor NR4A1 with GFPDepositorInsertNR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
UseCRISPRTagsExpressionMutationhg38:12:52058840:C>T, hg38:12:52058930:A>C,…PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3
Plasmid#112005PurposeFor enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3DepositorHas ServiceAAV5 and AAV9Insertaxon-GCaMP6s-P2A-mRuby3
UseAAVTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJEP300-pAAV-CMV-MCS2-pA
Plasmid#112138PurposeCMV driven Multi Cloning Site-2DepositorInsertN/A
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNR4A1.1.0-gDNA
Plasmid#112391PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1DepositorInsertNR4A1 (NR4A1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion Halo-mAID
Plasmid#112852PurposeGeneral donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous proteinDepositorInsertHalo-mAID
UseSynthetic BiologyTagsExpressionMutationPromoterNoAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-FLEx-axon-GCaMP6s
Plasmid#112010PurposeFor cre-dependent enriched expression of GCaMP6s in axonsDepositorHas ServiceAAV5 and AAV9Insertaxon-GCaMP6s
UseAAV and Cre/LoxTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV_BE4max_3xHA
Plasmid#112096PurposeC:G-to-T:A base editingDepositorInsertBE4max_3xHA
UseCRISPRTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMito.iGABASnFR
Plasmid#112175PurposeGABA SensorDepositorInsertiGABASnFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn-FLEX.iGABASnFR
Plasmid#112163PurposeGABA SensorDepositorHas ServiceAAV1InsertiGABASnFR
UseAAVTagsExpressionMutationPromoterSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2
Plasmid#112837Purposetransient/stable overexpressionDepositorInsertGrb2 (Grb2 Mouse)
UseTagsGFPExpressionMammalianMutationSH2 + SH3 (C-term) domains of Grb2PromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W
Plasmid#112927PurposeCRISPR-a reporter assay lentiviral vector (gTetO)DepositorInserthU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NS3-NLS/VPR
Plasmid#112244PurposeEncodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.DepositorInsertdCas9-NS3-NLS/VPR
UseTagsAU1 (N term on NS3) and HA (C term on NS3)ExpressionMammalianMutationNS3 mutant T54APromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
HIVGKO
Plasmid#112234PurposeHIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1aDepositorInsertcsGFP-EF1a-mKO2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
KRAB-dCas9
Plasmid#112195PurposeKRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertKRAB (ZNF10 Human)
UseCRISPRTags3XFLag-NLS-KRAB-dCas9-NLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX458-ECFP
Plasmid#112220PurposeCloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFPDepositorInsertECFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIGAhRC
Plasmid#112510Purposehuman Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoterDepositorUseSynthetic BiologyTagsExpressionYeastMutationPromoterGal1,10Available sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-3xflagGREB1a
Plasmid#112221Purposemammalian expression of flag tagged GREB1aDepositorInsertGREB1a (GREB1 Human)
UseTags3x flagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
hCathepsin S
Plasmid#11251PurposeMammalian expression of human Cathepsin SDepositorInsertcathepsin S (CTSS Human)
UseTagsC9 (GTETSQVAPA)ExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSAM_Bt
Plasmid#112497PurposeTransposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).DepositorInsertsINSeq-adapted mariner transposon
Himar1C9
UseTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only