We narrowed to 12,146 results for: 112

1. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

   Plasmid

   #112139

   Purpose

   Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.

   Depositor

   Erik Sontheimer

   Article

   Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:

   Inserts

   sgRNA scaffold
   Human-codon-optimized NmeCas9
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U1a and U6

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3

   Plasmid

   #112005

   Purpose

   For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV5 and AAV9

   Insert

   axon-GCaMP6s-P2A-mRuby3
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Promoter

   hSynapsin1

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPagP

   Plasmid

   #112125

   Purpose

   Bacterial expression of outer membrane protein PagP, wild type

   Depositor

   Karen Fleming

   Article

   Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

   Insert

   PagP (pagP E. coli)
   

   Expression

   Bacterial

   Mutation

   N-terminal signal sequence not included and repla…

   Promoter

   T7

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p006-Banana-Late

   Plasmid

   #112251

   Purpose

   ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   ATF1 under osmY promoter
   

   Expression

   Bacterial

   Promoter

   osmY

   Available Since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. eGFP-PRM-5R

   Plasmid

   #112155

   Purpose

   Mammalian expression plasmid for PRM-5R in a vector expressing eGFP.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   PRM-5R (ABL1 Human)
   

   Expression

   Mammalian

   Mutation

   contains only the PRM domain of Abl1

   Promoter

   CMV

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGG-NFIX1

   Plasmid

   #112702

   Purpose

   Expression of NFIX1 protein and GFP

   Depositor

   Richard Gronostajski

   Insert

   Nuclear Factor I X1 (Nfix Mouse)
   

   Tags

   GFP and HA

   Expression

   Mammalian

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMM534

   Plasmid

   #112530

   Purpose

   pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Insert

   luxCDABE
   

   Expression

   Bacterial

   Promoter

   PL(HrtO)

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSAM_Bt

   Plasmid

   #112497

   Purpose

   Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

   Depositor

   Jeffrey Gordon, Andrew Goodman

   Article

   Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

   Inserts

   INSeq-adapted mariner transposon
   Himar1C9
   

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-bleo IGF-1R

   Plasmid

   #11212

   Depositor

   Ronald Kahn

   Article

   Entingh-Pearsall et al J Biol Chem. 2004 Sep 3. 279(36):38016-2

   Insert

   IGF-1R (IGF1R Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJC25NM

    Plasmid

    #1126

    Depositor

    Susan Lindquist

    Article

    Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

    Insert

    NM stop (SUP35 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pMLS7-egfp

    Plasmid

    #112917

    Purpose

    Fluorescence microscopy

    Depositor

    Miguel Valvano

    Article

    Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

    Insert

    eGFP
    

    Expression

    Bacterial

    Promoter

    PS7 promoter and Ps7

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL7 lenti-dcas9-3xflag-blast

    Plasmid

    #112133

    Purpose

    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    dCas9(D10A, N863A)-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A mutants in Cas9

    Promoter

    EF1a

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS420

    Plasmid

    #11259

    Depositor

    Fred Cross

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX458-DsRed2

    Plasmid

    #112219

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    DsRed2
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    DsRed2 contains a silent nucleotide substitution …

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKC-4.04

    Plasmid

    #112085

    Purpose

    Control for NMD sensor: pCMV-3XFLAG-Fluc-betaglobin

    Depositor

    James Inglese

    Article

    Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1

    Insert

    3XFLAG firefly luciferase-beta-globin (HBB Human, Photinus pyralis, Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKC-4.06

    Plasmid

    #112084

    Purpose

    NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)

    Depositor

    James Inglese

    Article

    Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1

    Insert

    3XFLAG firefly luciferase-beta-globin 39PTC (HBB Human, Photinus pyralis, Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLIK CA RhoA

    Plasmid

    #112894

    Purpose

    promoter-inducible expression of constitutively-active RhoA

    Depositor

    Sanjay Kumar

    Article

    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

    Insert

    CA RhoA (RHOA Human)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. hCathepsin S

    Plasmid

    #11251

    Purpose

    Mammalian expression of human Cathepsin S

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

    Insert

    cathepsin S (CTSS Human)
    

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pCMV_BE4max_3xHA

    Plasmid

    #112096

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max_3xHA
    

    Use

    CRISPR

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pR26-CAG-H2B-miRFP703

    Plasmid

    #112853

    Purpose

    Donor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell lines

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    H2B-miRFP703
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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