Addgene

Showing: 81 - 100 of 2899 results

Results per page:

Sort results by:

pALS186 (ttTi5605-MCS loxP::C.br.unc-119(+)::loxP)

Plasmid

#129539

Purpose

modified pCFJ151 for C. elegans mosSCI at ttTi5605 or compatible sites; floxed C. br. unc-119(+) selection

Depositor

Arneet Saltzman

Article

Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

Type

Empty backbone

Use

Cre/Lox; Mos1-mediated single copy insertion (mos…

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBac(3xP3-gTc’v; pUb:lox-mYFP-lox-H2BmCherry)

Plasmid

#119064

Purpose

Cre/lox flip-out construct for marking cell clones in Tribolium castaneum

Depositor

Michalis Averof

Article

Gilles et al Development. 2019 Jan 4;146(1). pii: dev

Inserts

pBacL
pUb regulatory sequence
loxN
mYFP
loxN
H2B-mcherry
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

Plasmid

#227774

Purpose

Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

loxP-dsR-loxP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP

Plasmid

#204729

Purpose

Cre-inducible CRISPRi by Zim3-dCas9

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Zim3-dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

Plasmid

#196877

Purpose

Neuron-specific expression of mOrange2 reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTopo RE2 Donor pLox hPGK-Puro pA pLox

Plasmid

#171049

Purpose

Donor template for CRISPR/Cas9 targeting of TERT RE2

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Puromycin

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTopo RE3 Donor pLox hPGK-Puro pA pLox

Plasmid

#171050

Purpose

Donor template for CRISPR/Cas9 targeting of TERT RE3

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Puromycin

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-loxP-3xpA-loxP-sfGFP+HsbG syntron

Plasmid

#172477

Purpose

sfGFP with a synthetic intron derived from the human beta-globin gene

Depositor

Mario Capecchi

Insert

sfGFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-loxP-3xpA-loxP-sfGFP+pCI syntron

Plasmid

#172478

Purpose

sfGFP with a synthetic intron from the pCI expression vector (Promega)

Depositor

Mario Capecchi

Insert

sfGFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-loxP-3xpA-loxP-sfGFP+MmIghe syntron

Plasmid

#172475

Purpose

sfGFP with a synthetic intron derived from the mouse Ighe gene

Depositor

Mario Capecchi

Insert

sfGFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-loxP-3xpA-loxP-sfGFP+RbG syntron

Plasmid

#172476

Purpose

sfGFP with a synthetic intron derived from the rabbit beta-globin gene

Depositor

Mario Capecchi

Insert

sfGFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pB6-Id1-IRES-loxP-NeoR-loxP-DTA-TK

Plasmid

#158616

Purpose

B6J Id1-LSL-DTA allele targeting vector, low copy number

Depositor

Mario Capecchi

Insert

Id1 (Id1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Insertion of IRES-loxP-NeoR-loxP-DTA after the st…

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW758_pCAG-PV1-VloxP-FRT-lox2272-NeoR-BGHpA

Plasmid

#87580

Purpose

PV1 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

Neomycin Resistance Gene

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

Plasmid

#106171

Purpose

cre mediated red to green shift

Depositor

Ewa Snaar-Jagalska

Article

Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

Inserts

dsRED-express2
eGFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

Plasmid

#24334

Depositor

Didier Stainier

Article

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

H2B

Use

Cre/Lox

Tags

GFP

Expression

Worm

Mutation

mCherry driven by an HSP70 promoterCerulean drive…

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

Plasmid

#180152

Purpose

tdTomato cre reporter with Rosa26 recombineering homology arms

Depositor

Mario Capecchi

Insert

tdTomato

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-lox-rox-STOP-rox-tRFP-pA-lox-ZsGreen-pA

Plasmid

#175438

Purpose

dual plasmid labelling cells of different neuronal population with different fluorophores

Depositor

Sebastián Dupraz, Frank Bradke

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

rox-STOP-rox-tRFP-pA

Use

Cre/Lox; Dre/rox

Tags

ZsGreen on backbone

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

Plasmid

#85041

Purpose

This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

loxP-stop-loxP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

Plasmid

#52419

Purpose

Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

ERAS (ERAS Human)

Use

Tags

Expression

Mutation

Promoter

CAGGS

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

Plasmid

#162164

Purpose

Selectable homology donor template for Aag2 cell AGO1

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

RFP
3xFLAG

Use

CRISPR and Cre/Lox; Homology donor template

Tags

AGO1 homology and loxP

Expression

Insect

Mutation

Promoter

Ae. aegypti PUb and endogenous AGO1

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge