We narrowed to 2,682 results for: KL

1. pBLO 62.5

   Plasmid

   #123124

   Purpose

   Human expression vector for planctomyces CasX and sgRNA

   Depositor

   Jennifer Doudna, Benjamin Oakes

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Inserts

   CasX protein
   CasX sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA-merlin

   Plasmid

   #32836

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   Merlin (NF2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pBabepuro3-p16Flag

   Plasmid

   #24934

   Depositor

   Lynda Chin

   Article

   Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

   Insert

   p16 (CDKN2A Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL3-KRAS

   Plasmid

   #13919

   Depositor

   Frank Slack

   Article

   Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

   Insert

   KRAS 3'UTR (KRAS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR223-VPS35

   Plasmid

   #21689

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Vacuolar Protein Sorting 35 homolog (VPS35 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   last nucleotide of stop codon removed for c-termi…

   Promoter

   Available since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pRK5 FLAG wildtype, catalytic dead lipin 1

   Plasmid

   #32006

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   Lipin1 (Lpin1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D712E and D714E

   Promoter

   CMV, SP6

   Available since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pCAX-mScarlet-FBP17

   Plasmid

   #178366

   Purpose

   Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells

   Depositor

   Erik Dent

   Article

   Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

   Insert

   Formin Binding Protein 17 (FNBP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   400 Glutamic Acid changed to Aspartic acid (403 A…

   Promoter

   Chicken Beta Actin

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. TK-GFP-Nluc-P2A-neo-TK

   Plasmid

   #134896

   Purpose

   Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Insert

   TK-GFP-Nluc-P2A-Neo-TK
   

   Use

   Cryptosporidium expression

   Tags

   Expression

   Mutation

   Promoter

   Cryptosporidium actin and enolase

   Available since

   Nov. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5-FRT/TO-N-mEGFP-PARP6

   Plasmid

   #178005

   Purpose

   Expresses N-terminally mEGFP-tagged PARP6

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP6 (PARP6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-FRT/TO-N-mEGFP-PARP11

    Plasmid

    #177998

    Purpose

    Expresses N-terminally mEGFP-tagged PARP11

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP11 (PARP11 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS

    Plasmid

    #113436

    Purpose

    Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    type II-C anti-CRISPR
    

    Use

    CRISPR

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO-puro FLAG wildtype, catalytic active lipin 1

    Plasmid

    #32010

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 (Lpin1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pCsy_complex

    Plasmid

    #89232

    Purpose

    Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

    Depositor

    Blake Wiedenheft

    Article

    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

    Inserts

    Csy1
    Csy2
    Csy3
    Csy4
    

    Use

    Tags

    6xHis-TEV protease

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBLO 64.1

    Plasmid

    #123120

    Purpose

    E. coli expression vector for active deltaproteobacteria CasX

    Depositor

    Jennifer Doudna, Benjamin Oakes

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    CasX
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    NA

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Danio alpha A-crystallin 1kb promoter/AcGFP

    Plasmid

    #98096

    Purpose

    aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
    

    Use

    Gfp expressing

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Danio alpha A-crystallin 1 kb fragment (-1028/-1)

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR

    Plasmid

    #136514

    Purpose

    Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vector

    Depositor

    Erik Sontheimer

    Article

    Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

    Insert

    Codon-optimized AcrIIA5
    

    Use

    Lentiviral

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pACEBac1-PARP1-Flag-His-7xKQ

    Plasmid

    #111573

    Purpose

    Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Mutation

    lysine to glutamine mutation at the following ami…

    Promoter

    polyhedrin promoter

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tdKatushka2-Fibrillarin-7

    Plasmid

    #56041

    Purpose

    Localization: Nucleoli, Excitation: 586, Emission: 632

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    tdKatushka2

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pBLO ddd64.1

    Plasmid

    #123121

    Purpose

    E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

    Depositor

    Jennifer Doudna, Benjamin Oakes

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    CasX
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Mutated amino acids in catalytic triad to alanine…

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pblic-neo TRX1

    Plasmid

    #110919

    Purpose

    To overexpress Thioredoxin-1

    Depositor

    Priyamvada Rai

    Article

    Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

    Insert

    TRX1 (TXN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNAintron-CHIKV-Structural polyprotein

    Plasmid

    #215698

    Purpose

    Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV structural polyprotein Capsid-E3-E2-6K-E1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pMH-SFB-DYRK1B

    Plasmid

    #101771

    Purpose

    Mammalian expression of DYRK1B fusion protein

    Depositor

    Michael Huen

    Article

    Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

    Insert

    DYRK1B / MIRK (DYRK1B Human)
    

    Use

    Tags

    S peptide-flag-streptavidin binding peptide (SFB)

    Expression

    Mammalian

    Mutation

    siRNA-resistant allele

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

23. MIGR1-cyclin E-AA

    Plasmid

    #47498

    Purpose

    MSCV-based vector encodes human cyclin E mutated within N- and C-terminal Cdc4 phosphodegrons

    Depositor

    Alex Minella

    Article

    Xu et al Oncogene. 2013 Jul 22. doi: 10.1038/onc.

    Insert

    cyclin E1 (CCNE1 Human)
    

    Use

    Retroviral; Mscv-ires-gfp

    Tags

    Expression

    Mammalian

    Mutation

    threonines 62 and 380 to alanine (T62A T380A)

    Promoter

    LTR

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pET28-HADH2

    Plasmid

    #67863

    Purpose

    bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    HADH2 (HSD17B10 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDH-EFS-FlpO

    Plasmid

    #191509

    Purpose

    Lentiviral Flp Recombinase driven by the EFS promoter

    Depositor

    Eric Snyder

    Article

    Orstad et al Dev Cell. 2022 Aug 8;57(15):1866-1882.e1

    Insert

    flp recombinase (flp )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/EFS promoter inserted

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. integrin alpha9 pcDNA1 neo

    Plasmid

    #13585

    Depositor

    Dean Sheppard

    Article

    Yokosaki et al J Biol Chem. 1994 Oct 28. 269(43):26691-

    Insert

    integrin alpha 9 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

    Plasmid

    #110300

    Purpose

    Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

    Depositor

    Chris Kok-Lung Chan

    Article

    Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMSCV_Mock

    Plasmid

    #223565

    Purpose

    The plasmid is expressed in mammalian cells as a control.

    Depositor

    Hyun Woo Park

    Article

    Shin et al Mol Cancer. 2023 Nov 6;22(1):177. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEBG-bZIPa

    Plasmid

    #12436

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

    Insert

    bZIP of C/EBPa (CEBPA Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    pEBG vector is from Bruce Mayer, may be different…

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

30. tdKatushka2-CENPB-N-22

    Plasmid

    #56033

    Purpose

    Localization: Nucleus/Centromeres, Excitation: 586, Emission: 632

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Insert

    CENPB (CENPB Human)
    

    Use

    Tags

    tdKatushka2

    Expression

    Mammalian

    Mutation

    aa 1-169 of CENPB (DNA Binding Domain)

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits