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Plasmid#202733Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only
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TFORF1602
Plasmid#143139PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF256 (ZNF256 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B15-VN
Plasmid#135514PurposeMammalian expression of VN-fused and myc-tagged HLA-B*15:01DepositorInsertHLA-B*15:01 (HLA-B Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B57-VN
Plasmid#135515PurposeMammalian expression of VN-fused and myc-tagged HLA-B*57:01DepositorInsertHLA-B*57:1 (HLA-B Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Dd (Y84C/C121S/A139C)
Plasmid#136063PurposeMammalian expression of myc-tagged H2-Dd (Y84C/C121S/A139C mutant)DepositorInsertH2-Dd (H2-D1 Mouse)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationY84C/C121S/A139CPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B8-VN
Plasmid#135511PurposeMammalian expression of VN-fused and myc-tagged HLA-B*08:01DepositorInsertHLA-B*08:01 (HLA-B Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
dre-cdk2 3'UTR sicheck2
Plasmid#121123Purposeluciferase assayDepositorInsertdre-cdk2 3'UTR (cdk2 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoBb.1
Plasmid#121898PurposePlasmid for making zebrafish apoBb.1 in situ probeDepositorInsertApoBb.1 (apobb.1 Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-IVb.1
Plasmid#121903PurposePlasmid for making zebrafish apoA-IVb.1 in situ probeDepositorInsertApoa4b.1 (apoa4b.1 Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-IVb.2
Plasmid#121904PurposePlasmid for making zebrafish apoA-IVb.2 in situ probeDepositorInsertApoa4b.2 (apoa4b.2 Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
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exon K-renilla
Plasmid#81007Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila paraylticDepositorInsertparalytic (para Fly)
UseTagsRenilla luciferaseExpressionInsectMutationspanning exon 24 to exon 26, termination codon in…PromoterP-AC5 (Actin 5C)Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-KANK3
Plasmid#69238PurposeU6 driven SpCas9 sgRNA expression for KANK3 siteDepositorInsertKANK3 sgRNA (KANK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP
Plasmid#65846PurposeMammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorInsertTransmembrane (23 AA) of UQCC3 (UQCC3 Human)
UseTagsGFPExpressionMammalianMutationR5A-K6APromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP
Plasmid#65847PurposeMammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorInsertTransmembrane (23 AA) of UQCC3 (UQCC3 Human)
UseTagsGFPExpressionMammalianMutationR5D-K6DPromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-WRAP53beta-siRNA-resistant
Plasmid#64680Purposeexpresses siRNA resistant WRAP53betaDepositorInsertWD40-encoding RNA antisense to P53 (WRAP53 Human)
UseTagsFLAGExpressionMammalianMutationsiRNA resistantPromoterCMVAvailable sinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_LRRC31
Plasmid#45271DepositorInsertLRRC31 (LRRC31 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pUT1148-HQ858692
Plasmid#27738DepositorInsertZmMYB8 transcription factor from AM156905 (myb8 Zea mays)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase INF enhancer vector_ORI_MX1
Plasmid#99323PurposeLuciferase validation vector with MX1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr21: 42791443 -42793515
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
RANLS-DEVD-BNES
Plasmid#50840PurposeExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddRFP A and ddRFP B
UseTagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
GANLS-DEVD-BNES
Plasmid#50835PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddGFP A and ddRFP B
UseTagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Integrin beta5-2XEGFP
Plasmid#139779PurposeDetecting Integrin beta5 by fluorescence microscopyDepositorInsertIntegrin subunit beta5 (ITGB5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMarsL274G
Plasmid#63177PurposeThis plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.DepositorInsertMarsL274G (Mars1 Mouse)
UseTagsExpressionMammalianMutationL274GPromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
MC4R-Tango
Plasmid#66430PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertMC4R (MC4R Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GCGR-DuET
Plasmid#213250PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGCGR (GCGR Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLIK CA ROCK2
Plasmid#84649PurposeLentiviral expression of doxycycline-inducible constitutively active ROCK2 and co-expression of VenusDepositorInsertROCK2 (ROCK2 Bovine)
UseLentiviralTagsExpressionMammalianMutationConstitutive Active (please see depositor comment…PromoterAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBMN FKBP(DD)-YFP
Plasmid#31763DepositorInsertFKBP(DD)-YFP-HA (FKBP1A Human)
UseRetroviralTagsHA and YFPExpressionMutationF36V, L106P on FKBP12PromoterLTRAvailable sinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
ADRB3-Tango
Plasmid#66221PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertADRB3 (ADRB3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GCGR-Tango
Plasmid#66291PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGCGR (GCGR Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
ADRB3-DuET
Plasmid#213182PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertADRB3 (ADRB3 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 asyn WT
Plasmid#107425PurposeMammalian expression of human alpha synucleinDepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-RVG-10-Lamp2b-HA
Plasmid#71294PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsGNSTM glycosylation motif, HA, Lamp2 signal pepti…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Utr230-EGFP-3XNLS
Plasmid#58466PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoterDepositorInsertsUseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only