We narrowed to 18,496 results for: Met

1. pBITE-EGFRvIII-F269-OKT3

   Plasmid

   #190681

   Purpose

   Encodes an EGFRvIII-CD3 BITE protein (F269 clone)

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-TCF1

   Plasmid

   #40620

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

   Insert

   TCF7 (TCF7 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-NLS-AcrIIA4

   Plasmid

   #113037

   Purpose

   Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SV40 NLS-AcrIIA4
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSNAP-CLC-SNAP

   Plasmid

   #38012

   Depositor

   Xiaowei Zhuang

   Article

   Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

   Insert

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   

   Tags

   SNAP26m

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBITE-EGFRvIII-F263-OKT3

   Plasmid

   #190680

   Purpose

   Encodes an EGFRvIII-CD3 BITE protein (F263 clone)

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138131

   Purpose

   Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mCherry-anti-rabbit-IgG(Fc)-nanobody
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRVL-5

   Plasmid

   #104583

   Purpose

   Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

   Depositor

   Daniel Christ

   Article

   Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

   Insert

   Human IgG1 CH1/CH2/CH3 (IGHG1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Syn-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

   Plasmid

   #222742

   Purpose

   CaST-IRES for neuron expression

   Depositor

   Christina Kim

   Article

   Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

   Insert

   CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPRa_VPR_yl

   Plasmid

   #107677

   Purpose

   CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

   Inserts

   Codon optimized dCas9-VPR
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

    Plasmid

    #175523

    Purpose

    Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    LAMP1-HaloTag11 and Tomm20-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDx_mScarlet3-SYFP2

    Plasmid

    #189764

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYDS2.0_AT118-L

    Plasmid

    #222847

    Purpose

    Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Expression

    Yeast

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pgRNA-CGG

    Plasmid

    #108248

    Purpose

    Lentiviral vector to express sgRNA targeting "GGCGGCGGCGGCGGCGGCGG" locus by SpCas9

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

    Insert

    sgRNA targeting GGCGGCGGCGGCGGCGGCGG
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-FLAG LAP2

    Plasmid

    #15738

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    LAP2 (CEBPB Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLAMP1-miRFP670nano3

    Plasmid

    #184669

    Purpose

    LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    LAMP1-miRFP670nano3
    

    Tags

    miRFP670nano3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFR-mApple

    Plasmid

    #203773

    Purpose

    Number and brightness analysis of EGFR oligomerization

    Depositor

    Thorsten Wohland

    Article

    Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

    Insert

    EGFR (EGFR Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p46Cpf1-OP2

    Plasmid

    #98592

    Purpose

    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.

    Depositor

    Qiong Wu

    Article

    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:

    Insert

    FnCpf1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    araBAD

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE-puro LEF1

    Plasmid

    #27023

    Depositor

    Joan Massague

    Article

    Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

    Insert

    LEF1 (LEF1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSMPP-mCherry-mTrim21

    Plasmid

    #104971

    Purpose

    Lentiviral Transfer Plasmid with mCherry-tagged mouse Trim21

    Depositor

    Leo James

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Tripartite Motif containing 21 (Trim21 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Promoter

    SFFV

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3/HA-FLAG-RBP2

    Plasmid

    #14800

    Depositor

    William Kaelin

    Article

    Klose et al Cell. 2007 Mar 9. 128(5):889-900.

    Insert

    Retinoblastoma Binding Protein 2 (KDM5A Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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