166,033 results

1. StrKDEL-IRES-ManII-mScarlet-i

   Plasmid

   #117274

   Purpose

   Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i

   Depositor

   David Stephens

   Article

   McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

   Inserts

   partial sequence of mannosidase II (Man2a1 Mouse)
   Streptavidin-KDEL
   

   Tags

   mScarlet-i

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV and IRES

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC1749

   Plasmid

   #127983

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_FLAG-Mx1201 PylRS

   Plasmid

   #154764

   Purpose

   plasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-HASAP

   Plasmid

   #138325

   Purpose

   chemigenetic voltage indicator

   Depositor

   Eric Schreiter

   Article

   Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

   Insert

   HASAP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCast

   Plasmid

   #200169

   Purpose

   E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

   Depositor

   Yong-Su Jin

   Article

   Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1

   Plasmid

   #122964

   Purpose

   Expresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 promoter. It can be used as a cloning vector for gene of interest at SfiI sites.

   Depositor

   FENG-QIAN Li, Ken-Ichi Takemaru

   Insert

   human Chibby1 (CBY1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Promoter

   FoxJ1 and human FoxJ1

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223-BLK

   Plasmid

   #23940

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   BLK (BLK Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

   Plasmid

   #171615

   Purpose

   Soma-targeted Cal-Light with KA2

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
   

   Use

   Synthetic Biology

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-HLA-A0201-His

   Plasmid

   #108213

   Purpose

   HLA-A0201 his tagged

   Depositor

   Sébastien Walchli

   Article

   Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

   Insert

   major histocompatibility complex, class I, B (HLA-A Human)
   

   Use

   Entry vector for gateway system

   Tags

   his

   Available Since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(Gq)

    Plasmid

    #230015

    Purpose

    Expresses anti-TNFalpha PAGER(Gq) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-Ozoralizumab-TEVcs-ALFA-hM1D
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    W442A on hM1D

    Promoter

    CMV

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD

    Plasmid

    #225703

    Purpose

    Lentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (K36M) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    K36M

    Promoter

    pEF1A

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSW002-Pc-DsRed-Express2

    Plasmid

    #111251

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    DsRed-Express2
    

    Expression

    Bacterial

    Promoter

    Pc

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. EIF2AK4 gRNA (BRDN0001146469)

    Plasmid

    #75876

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK4 (EIF2AK4 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. C1-FT-mCitrine-EEVEE-CaaX

    Plasmid

    #155225

    Purpose

    MPAct with increased linker flexiblity

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b
    

    Tags

    mCitrine

    Expression

    Mammalian

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPIDNB

    Plasmid

    #169901

    Purpose

    Empty dox inducible vector with piggyBac, insulators, and a DTS

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Aivian expression

    Expression

    Mammalian

    Promoter

    Dox inducible

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSW002

    Plasmid

    #111248

    Purpose

    Broad host-range bacterial vector

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBNP-NFAT-luciferase

    Plasmid

    #24956

    Depositor

    Toren Finkel

    Insert

    BNP promoter (NPPB Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pF-N-HiBiT-template

    Plasmid

    #200882

    Purpose

    template for N-terminal HiBIT-tagging

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    HiBIT-linker
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-JAK3(M511)_IRES_GFP

    Plasmid

    #206290

    Purpose

    Expression of mutant JAK3 (M511I) that is able to drive constitutive activation of the JAK/STAT pathway

    Depositor

    Charles de Bock

    Article

    Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

    Insert

    JAK3 (M511I)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Methionine 511 to Isoleucine

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAS_FLAG-G1 PylRS

    Plasmid

    #154763

    Purpose

    plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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