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170,823 results

Showing: 1001 - 1020 of 170823 results

  2. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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  4. pEM047 [T7-assisted CROPseq sgRNA expression vector]

    Plasmid
    #224899
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA
    Depositor
    Thomas Norman
    Article
    Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    224899-plasmid-map-sequence-id-443624

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  12. pSBbi-BN

    Plasmid
    #60518
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60518-plasmid-map-sequence-id-97528

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  18. mEmerald-Fascin-C-10

    Plasmid
    #54094
    Purpose
    Localization: Actin Bundling Protein, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Fascin (FSCN1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. IDG_CACNA2D2_OE_1

    Plasmid
    #161616
    Purpose
    Overexpresses CACNA2D2
    Depositor
    Mike McManus
    Insert
    CACNA2D2 (CACNA2D2 Human)
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    161616-plasmid-map-sequence-id-318722

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  20. Lenti-EF1alpha-ZsGreen1_Puro

    Plasmid
    #192661
    Purpose
    3rd generation lenti vector encoding ZsGreen1 with 2A Puro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    ZsGreen1
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    192661-plasmid-map-sequence-id-379918

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Showing: 1001 - 1020 of 170823 results