We narrowed to 43,772 results for: tra
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pQCascade(Ptr)-entry(BsaI)
Plasmid#175579PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, CRISPR(BsaI)
ExpressionBacterialMutationNOAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
MF82_PB-TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
Plasmid#176626PurposeTF A self-activation cassette in MultiFate-2.2 and MultiFate-3DepositorInsertTRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
UseSynthetic BiologyAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV GFAP jGCaMP7b
Plasmid#171118PurposeAstrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoterDepositorInsertjGCaMP7b
UseAAVTags6 His Tag, T7 Tag, and Xpress TagExpressionMammalianPromoterGFAPAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I tension sensor (F40-based)
Plasmid#119186PurposeThe F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.DepositorInserthuman Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hTRF2
Plasmid#103804PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Puro- MLL3-PRKAG2
Plasmid#69473PurposeExpresses MLL3-PRKAG2 fusion geneDepositorAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRevTRE MKL1 S449A/T450A/S454A
Plasmid#19847DepositorInsertMKL1 S449A/T450A/S454A (MRTFA Human)
UseRetroviralTags3xFLAGMutationchanged Serine 449, Threonine 450 and Serine 454 …Available SinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP2
Plasmid#129500PurposeExpresses AausFP2 constitutively in E. coli (most strains)DepositorInsertAausFP2
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRN119
Plasmid#176884PurposeEncodes STM and WUS2 for ectopic meristem induction in plantsDepositorInsertCmYLCV:Luc+:HSP, Nos::WUS2:pinII, and 35:STM:35S
ExpressionPlantAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-NET-myc
Plasmid#107458PurposeMammalian expression plasmid for codon optimized hNET with internal MYC tag inserted in ECL2DepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-DAT-myc
Plasmid#107457PurposeMammalian expression plasmid for codon optimized hDAT with internal MYC tag inserted in ECL2DepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HSPA5P
Plasmid#44150Purposea fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)DepositorInsertHSPA5 promoter (HSPA5 Human)
ExpressionMammalianAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)
Plasmid#213027PurposeExpresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
Tags3xFlag (ORF2p)ExpressionMammalianMutationD702Y (RT- reverse transcriptase catalytic dead)PromoterCMVAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRM18 LTN (Luciferase tdTomato Neo)
Plasmid#174721PurposeExpresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.DepositorInsertsFirefly Luciferase
tdTomato
aph
UseLentiviral and LuciferaseTagsE2A linker and P2A linkerExpressionMammalianPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)
Plasmid#217808PurposeExpression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)DepositorInsert5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterT7Available SinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI
Plasmid#106895PurposeExpresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulationDepositorInsertsGAI-tagBFP-Sp dCas9-ABI
GID1-KRAB
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …ExpressionMammalianPromoterCAG and PGKAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluf
Plasmid#105833PurposeFast indicator for extracellular glutamate imagingDepositorInsertiGluSnFR E25D variant
TagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromoterCMVAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag_MmPERK_S503_N1114_pBabePuro
Plasmid#58423Purposeretroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.DepositorInsertPERK (Eif2ak3 Mouse)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationDeleted amino acids M1 to Y502Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.28H.28TM.BB.28.3z
Plasmid#200681PurposeModular CD28hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianPromoterEF1a-shortAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only
