We narrowed to 18,759 results for: nls
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Plasmid#244841PurposeBacterial expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminusDepositorInsertLbCas12a-RVQ
UseCRISPRTags2xSV40 NLS and 10xHis-CL7-2xSV40 NLSExpressionBacterialMutationG146R/R182V/E795QPromoterT7Available sinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-NLStdMCP- tagRFP
Plasmid#86244PurposeMiddle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-tagRFPDepositorTypeEmpty backboneUseUnspecifiedTagsNLS-tdMCP-tagRFPAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAd-PV-NLS-CD-DsRed
Plasmid#16345DepositorInsertPV-DsRed-NLS-CD (Pvalb Rat)
UseAdenoviralTagsMycExpressionMammalianMutationFunctional Ca2+binding site CD was inactivated by…Available sinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DL031_pAAV_fos_HaloTag_NLS
Plasmid#187450PurposeAAV expression vector for NLS-halo tagDepositorInsertHALO tag
UseAAVTagsNLSExpressionMammalianAvailable sinceOct. 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
JAB3616
Plasmid#190426PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::TetR-NLS-19St_proT::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::TetR-NLS-19St_proT::GFPint-ADHt
ExpressionPlantAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2601
Plasmid#190388PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt
ExpressionPlantAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2600
Plasmid#190387PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt
ExpressionPlantAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2599
Plasmid#190386PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt
ExpressionPlantAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-ExRai-AktAR2
Plasmid#184049PurposeNuclear-targeted ExRai-AktAR2.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNuc-ExRai-AktAR2
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-NLS-tdMCP-eGFP
Plasmid#86243PurposeMiddle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-eGFP reporterDepositorTypeEmpty backboneUseUnspecifiedTagsNLS-tdMCP-eGFPAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET42b-A3A-PBE-ΔUGI
Plasmid#119771PurposeTo express the recombinant APOBEC3A in vitroDepositorInsertAPOBEC3A-nCas9-NLS
Tags6*HIS and NLSExpressionBacterialMutationnCas9 has a mutation D10A in wild type SpCas9PromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP
Plasmid#188902PurposedCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertdCas9-KRAB(Kox1)-MeCP2
UseLentiviralTagsNLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…PromoterSFFVAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-NLS-actin-R62D
Plasmid#118381PurposeGateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.DepositorAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-NLS-MaSat ZF-18-mVenusN
Plasmid#191392PurposeZinc finger fused to N-terminal part of split mVenusDepositorInsertFlag-NLS-MaSat ZF-18-mVenusN
Tags3xFLAGExpressionMammalianPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRvUBC-mEGFP-NLS
Plasmid#205010PurposeThe vector contains 1kbp of the upstream and downstream regions of the ubiquitin c gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ubiquitin c gene from R. varieornatus
UseTardigrade expressionAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL7 lenti-dcas9-3xflag-blast
Plasmid#112133Purposelenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)DepositorInsertdCas9(D10A, N863A)-T2A-Blast
UseCRISPR and LentiviralExpressionMammalianMutationD10A and N863A mutants in Cas9PromoterEF1aAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAR015 Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD
Plasmid#195544PurposedCas12a effector fusion for manipulating transcriptionDepositorInsertLenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD
UseCRISPR and LentiviralTags3XHAExpressionMammalianPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LacI-NLS-VP16
Plasmid#103836PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arraysDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-LacI-NLS-VP16
ExpressionMammalianPromoterSV2Available sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRhobin2-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS
Plasmid#239020PurposeCo-overexpression of Rhobin2-H2B-mNeonGreen3K fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling markerDepositorInsertRhobin2-H2B (H2BC21 Human, Synthetic)
TagsTagBFP and mNeonGreen3KExpressionMammalianPromoterCMV promoterAvailable sinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRhobin9-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS
Plasmid#239021PurposeCo-overexpression of Rhobin9-H2B-mNeonGreen3K fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling markerDepositorInsertRhobin9-H2B (H2BC21 Human, Synthetic)
TagsTagBFP and mNeonGreen3KExpressionMammalianPromoterCMV promoterAvailable sinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUAS5x:GFPNLS-SV40pA
Plasmid#155122PurposeTol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertQUAS5x:GFPNLS
UseZebrafish expressionPromoterQUAS5x-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
JAB3494
Plasmid#190418PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_<<2XAtMAR10>> 6XAO-2XPO::TetR-NLS-19Ster_proT::GFPint_ADHter )DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_<<2XAtMAR10>> 6XAO-2XPO::TetR-NLS-19Ster_proT::GFPint_ADHter
ExpressionPlantAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGal80(opt)
Plasmid#51618Purposevertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stopDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGal80(opt) (GAL80 Budding Yeast)
UseVertebrate expressionTagsNLSMutationcodon optimizedAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-mCherry-NLS
Plasmid#194049PurposeTransient expression of mCherry-NLS in plant cell (Nucleus)DepositorInsertmCherry
TagsSV40 (Nuclear localization signal)ExpressionPlantPromoterCaMV 35S promoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-GIB-Cre-N-WPRE_pKG3943
Plasmid#246352PurposeGIB-Cre Retrovirus expressing N-terminal giberellin inducible CreDepositorInsertiCre-N229-L1-GID1-NLS
UseRetroviralExpressionMammalianMutationnoneAvailable sinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-GIB-Cre-C-WPRE_pKG3944
Plasmid#246353PurposeGIB-Cre Retrovirus expressing C-terminal giberellin inducible CreDepositorInsertGAI-L1-iCre-230C-NLS
UseRetroviralExpressionMammalianMutationnoneAvailable sinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-dFnCas9
Plasmid#201954PurposeMammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-dFnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationD11A and H969A on FnCas9PromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pROF018
Plasmid#155325PurposeConstitutive expression of KRAB-EL222 in plantsDepositorInsertPCaMV35S-NLS-KRAB-EL222-Tnos
UseSynthetic BiologyExpressionPlantAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF050
Plasmid#155327PurposeConstitutive expression of BRD-EL222 in plantsDepositorInsertPCaMV35S-BRD-NLS-EL222-Tnos
UseSynthetic BiologyExpressionPlantAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1P56
Plasmid#195935PurposeLevel 1 PhiC31 cassette with constitutive promoter : p35S:UbGln:PhiC31-NLS:tUBQ1DepositorInsertp35S:UbGln:PhiC31-NLS:tUBQ1
ExpressionPlantAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT22UbGfpnE4PCh(#301)
Plasmid#184066Purposecyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenicDepositorInserteGFP-nls-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)MutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_3XNLS-MicroID2-FLAG
Plasmid#188523PurposeEncodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3XNLS-MicroID2
UseTransposon mediated gene integrationTagsFLAGExpressionMammalianPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiSAMPHv2
Plasmid#167934PurposeAll-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6, EF1aAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHes1(467)-luc
Plasmid#41723DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHes1 Promoter (-467 to +46) (Hes1 Mouse)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationContains the murine Hes1 promoter (-467 to +46)PromoterHes1 promoter fragment (-467 to +46)Available sinceMarch 20, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-BirA*-dCas9-eGFP
Plasmid#84779PurposeExpresses BirA*-dCas9-eGFP in mammalian cells.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBirA*-dCas9-eGFP
UseCRISPRTagscMycExpressionBacterial and MammalianPromoterCAGAvailable sinceJuly 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A
Plasmid#86674PurposeExpresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInsertGFP-FLAG-cGAS E225A/D227A
UseLentiviralAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL2-GAL4-UAS-Luc
Plasmid#33020DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert6X GAL4-UAS
TagsLuciferaseAvailable sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-evoCAST-evoTnsAB
Plasmid#234729PurposeEvolved human-codon optimized PseTnsAB in mammalian cells (for evoCAST)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertevoPseTnsAB
ExpressionMammalianAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3
Plasmid#171588PurposeLentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoterDepositorInsertsBxb1 integrase
iCasp9
BFP
rtTA3G
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCas9-sgAAVS1-2
Plasmid#129727PurposeExpressing SpCas9 and sgAAVS1-2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 and sgAAVS1-2
UseCRISPRTagsT2A-mCherry-P2A-PuroExpressionMammalianPromoterhU6Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by