We narrowed to 2,817 results for: ALC

1. pcDNA3_2FLAG-AKAP79ΔCN

   Plasmid

   #183451

   Purpose

   Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)

   Depositor

   Matthew Gold

   Article

   Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

   Insert

   AKAP5 (AKAP5 Human)
   

   Use

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   aa337-343 deleted

   Promoter

   CMV

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CaV2.1 (pCR3.1)

   Plasmid

   #26573

   Depositor

   Diane Lipscombe

   Article

   Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

   Insert

   Cacna1a (Cacna1a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pRSET a sfGCaMP6s

   Plasmid

   #100022

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   sfGCaMP6s
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   cpEGFP replaced with superfolder cpGFP

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMSP1D1R1

   Plasmid

   #97323

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Article

   Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

   Insert

   MSP1D1R1
   

   Use

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of R residue after T25

   Promoter

   T7

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSP1D1G1

   Plasmid

   #97325

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Article

   Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

   Insert

   MSP1D1G1
   

   Use

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of G residue after T25

   Promoter

   T7

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lentiviral CaMKKbeta

   Plasmid

   #33322

   Depositor

   Anthony Means

   Article

   Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   human PGK

   Available since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Ca-LiPPI-CLAPon1.0 (APEX)

   Plasmid

   #213408

   Purpose

   Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 1)

   Depositor

   Wenjing Wang

   Article

   Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

   Insert

   MK2-E5-truncated hlov-TEVcs-APEX(201-250)-K5-HA-HA-APEX(2-200)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lentiviral FLAG-CaMKKbeta

   Plasmid

   #32458

   Depositor

   Anthony Means

   Article

   Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pSG5-FLAG-CaMKKbeta rat FL

   Plasmid

   #32449

   Depositor

   Anthony Means

   Article

   Anderson et al Cell Metab. 2008 May;7(5):377-88.

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S3L, G96R, and P108L

   Promoter

   SV40

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pHAGE-eGFP-TAX1BP1 SKICH (1-140D)

    Plasmid

    #175768

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 SKICH (1-140D)

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    SKICH (1-140D)

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lentiviral CaMKKbeta K193A

    Plasmid

    #33323

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    K194A

    Promoter

    human PGK

    Available since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pMIEG3-NFAT2

    Plasmid

    #40351

    Depositor

    Alexander Dent

    Article

    Wang et al J Immunol. 2006 Jan 15;176(2):880-8.

    Insert

    NFAT2 (NFATC1 Mouse)
    

    Use

    Retroviral

    Tags

    IRES eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pSG5-HA-CaMKKbeta rat FL WT

    Plasmid

    #33321

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S3V, G96R, and P108L

    Promoter

    SV40

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA-SCW

    Plasmid

    #190633

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCW

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD-SCW

    Plasmid

    #190626

    Purpose

    Bacterial expression of genetically encoded calcium indicator SCW

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRII-Topo S100a10 in situ probe

    Plasmid

    #45603

    Depositor

    Jeffrey Macklis

    Article

    Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

    Insert

    s100a10 in situ probe (S100a10 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 21-275 of BC025044

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pET28a-His6-OAZ-GSY-SPM-Ctag

    Plasmid

    #163613

    Purpose

    Expresses truncated human Ornithine Decarboxylase Antizyme fused to SPM in bacterial cells for calcium-induced covalent coupling to Ornithine Decarboxylase.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    OAZ-GSY-SPM
    

    Use

    Tags

    C-tag and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSP1D1T1

    Plasmid

    #97327

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1T1
    

    Use

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of T residue after T25

    Promoter

    T7

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSG5-FLAG-CaMKKbeta rat K193A

    Plasmid

    #32450

    Depositor

    Anthony Means

    Article

    Anderson et al Cell Metab. 2008 May;7(5):377-88.

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K193A (S3V, G96R, and P108L also present)

    Promoter

    SV40

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCellFree_G03 CAMK1

    Plasmid

    #67075

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CAMK1 (CAMK1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pSG5-FLAG-CaMKKbeta rat D311A

    Plasmid

    #32451

    Depositor

    Anthony Means

    Article

    Anderson et al Cell Metab. 2008 May;7(5):377-88.

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D311A (S3V, G96R, and P108L also present)

    Promoter

    SV40

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

22. SBC015898

    Plasmid

    #226285

    Purpose

    Expresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.

    Depositor

    Rainer Breitling

    Article

    Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

    Inserts

    Coniferyl alcohol acyltransferase
    Eugenol synthase 1
    Eugenol O-methyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. (I) pADH2 (J) P3 (SBE114)

    Plasmid

    #187629

    Purpose

    promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (I) pADH2 (J)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. (F) pADH2 (G)_P2 (SBE113)

    Plasmid

    #187628

    Purpose

    promoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (F) pADH2 (G)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. (R)pADH2(D)_P1(SBE214)

    Plasmid

    #195015

    Purpose

    promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (B) pADH2 (R)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNMSB88

    Plasmid

    #199321

    Purpose

    Combine with Gal4 to direct TeNL expression

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    15xUAS::delta pes-10::::TeNL::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    KD for calcium is 250 nM, 1 synthetic intron

    Promoter

    15xUAS::delta pes-10

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNMSB109

    Plasmid

    #199322

    Purpose

    Combine with Gal4 to direct CaMBI 300 expression

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    15xUAS::delta pes-10::::CaMBI::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Orange CaMBI with 300 nM KD for calcium

    Promoter

    15xUAS:::delta pes-10

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHAGE-eGFP-TAX1BP1 Q770A E774K

    Plasmid

    #175770

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Q770A E774K

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Q770A E774K

    Promoter

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-RCC

    Plasmid

    #190635

    Purpose

    Mammalian expression of genetically encoded calcium indicator RCC

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    RCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA-SCcpV

    Plasmid

    #190636

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCcpV

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCcpV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHAGE-eGFP-TAX1BP1 632-639D

    Plasmid

    #175771

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 632-639D

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    632-639D

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSG5-FLAG-CaMKKbeta rat S572A

    Plasmid

    #33319

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S571A

    Promoter

    SV40

    Available since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pSG5-FLAG-CaMKKbeta with CaMKKalpha RP domain

    Plasmid

    #33332

    Depositor

    Anthony Means

    Article

    Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CaMKKbeta RP domain (203-225) replaced with CaMKK…

    Promoter

    SV40

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pSG5-FLAG-CaMKKalpha N-terminus on CaMKKbeta

    Plasmid

    #33329

    Depositor

    Anthony Means

    Article

    Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CaMKKalpha N-terminus(1-128) was fused with CaMKK…

    Promoter

    SV40

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pFSW Syt1-C2B D363,365N IRES GFP

    Plasmid

    #133822

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    D363,365N

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFSW Syt1-C2A D230,232N IRES GFP

    Plasmid

    #133824

    Purpose

    Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    D230,232N

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV.CAG.GCaMP6f.WPRE.SV40

    Plasmid

    #100836

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV2, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CAG

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGL4_CRE-CMVmin-luc2

    Plasmid

    #194384

    Purpose

    Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    6xCRE-CMVmin-luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV minimal

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV.CAG.GCaMP6s.WPRE.SV40

    Plasmid

    #100844

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    CAG

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40

    Plasmid

    #100835

    Purpose

    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CAG

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAAV.Syn.GCaMP6f.WPRE.SV40

    Plasmid

    #100837

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

    Plasmid

    #194683

    Purpose

    CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_05-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

    Plasmid

    #232880

    Purpose

    Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

    Depositor

    Paul DeCaen

    Article

    DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

    Insert

    ADP-ribosylation factor-like protein 13B (ARL13B Human)
    

    Use

    Lentiviral

    Tags

    GFP and RGECO1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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44. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40

    Plasmid

    #100833

    Purpose

    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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45. CaV2.1 (pcDNA6)

    Plasmid

    #26578

    Depositor

    Diane Lipscombe

    Article

    Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

    Insert

    Cacna1a (Cacna1a Mouse)
    

    Use

    Tags

    His6 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCMV 3xFLAG-LC3A WT

    Plasmid

    #123089

    Purpose

    Expresses 3xFLAG-LC3A wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Tol2-elavl3-GCaMP6s

    Plasmid

    #59531

    Purpose

    Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

    Depositor

    Misha Ahrens

    Article

    Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

    Inserts

    elavl3 promoter (elavl3 Zebrafish)
    GCaMP6s
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAV.Syn.GCaMP6s.WPRE.SV40

    Plasmid

    #100843

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCAG-mEGFP-CaMKIIa

    Plasmid

    #127389

    Purpose

    Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. CanlonicSF

    Plasmid

    #178342

    Purpose

    To explore lactate dynamics in intact systems

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    CanlonicSF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge