We narrowed to 29,328 results for: REP

1. pAIP4_6WEY

   Plasmid

   #234234

   Purpose

   Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAIP4_5HIH

   Plasmid

   #234205

   Purpose

   Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pAIP4_2H2Z

   Plasmid

   #234147

   Purpose

   Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCWU50

   Plasmid

   #232318

   Purpose

   A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch

   Depositor

   Chenggang Wu

   Insert

   Pfdx-E
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   fdx

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1203AE-CMV-Cas7.1-7.2-H43A-mutant

   Plasmid

   #221449

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, H to A mutation at amino acid 43 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 1203AF-CMV-Cas7.1-7.2-Y55A-mutant

   Plasmid

   #221450

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, Y to A mutation at amino acid 55 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 1203AG-CMV-Cas7.1-7.2-N152A-mutant

   Plasmid

   #221451

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, N to A mutation at amino acid 152 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUSE-URA3-Sec7-mEYFP

   Plasmid

   #218968

   Purpose

   Gene replacement plasmid to label S. cerevisiae Sec7 with mEYFP

   Depositor

   Benjamin Glick

   Insert

   SEC7-mEYFP (SEC7 Budding Yeast)
   

   Use

   Gene replacement

   Tags

   mEYFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUSE-URA-Sec7-mGold

   Plasmid

   #218967

   Purpose

   Gene replacement plasmid to label S. cerevisiae Sec7 with mGold

   Depositor

   Benjamin Glick

   Insert

   SEC7-mGold (SEC7 Budding Yeast)
   

   Use

   Gene replacement vector for yeast

   Tags

   mGold

   Expression

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pD(Nt)_TS-3C

    Plasmid

    #223883

    Purpose

    4G-cloning donor for N-terminal TwinStrep-3C tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    TwinStrep-3C
    

    Use

    Golden-gate donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pD(Ct)_TS

    Plasmid

    #223891

    Purpose

    4G-cloning donor for C-terminal TwinStrep tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    TwinStrep
    

    Use

    Golden-gate donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pD(Ct)_3C-TS

    Plasmid

    #223892

    Purpose

    4G-cloning donor for C-terminal 3C-TwinStrep tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    3C-TwinStrep
    

    Use

    Golden-gate donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YIplac211-Kar2-moxGFP2-HDEL

    Plasmid

    #218970

    Purpose

    Gene replacement plasmid to label S. cerevisiae Kar2 with moxGFP2

    Depositor

    Benjamin Glick

    Insert

    KAR2-moxGFP2-HDEL (KAR2 Budding Yeast)
    

    Use

    Gene replacement vector for yeast

    Tags

    moxGFP2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2RV

    Plasmid

    #193373

    Purpose

    Contains the scond TALE repeat harboring RV as RVD

    Depositor

    Shin-ichi Arimura

    Article

    Hosoda et al bioRxiv 2023.02.27.530312

    Insert

    Repeat 2 RV
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p3RV

    Plasmid

    #193374

    Purpose

    Contains the third TALE repeat harboring RV as RVD

    Depositor

    Shin-ichi Arimura

    Article

    Hosoda et al bioRxiv 2023.02.27.530312

    Insert

    Repeat 3 RV
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p4RV

    Plasmid

    #193375

    Purpose

    Contains the fourth TALE repeat harboring RV as RVD

    Depositor

    Shin-ichi Arimura

    Article

    Hosoda et al bioRxiv 2023.02.27.530312

    Insert

    Repeat 4 RV
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. R6K_tac_dGFPmut3_ChInt

    Plasmid

    #187388

    Purpose

    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)

    Depositor

    Roi Avraham

    Insert

    dGFPmut3_AAV
    

    Use

    Tags

    AAV

    Expression

    Bacterial

    Mutation

    C-terminal AAV tail

    Promoter

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. iRFP670 - H2A

    Plasmid

    #176092

    Purpose

    nuclear marker

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al bioRxiv 2022.11.24.517828

    Insert

    H2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZDonor-U6-myogenin stop codon gRNA

    Plasmid

    #69549

    Purpose

    Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Insert

    Myogenin C terminus-targeting guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGMCK-P38-luxAB-DAS+4

    Plasmid

    #27376

    Depositor

    Dirk Schnappinger

    Article

    Klotzsche et al Nucleic Acids Res. 2009 Jan 27. ():.

    Insert

    luxA, luxBct6
    

    Use

    Gateway destination vector

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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