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pQFBD

Plasmid

#165823

Purpose

QF DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

QFBD

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQFAD

Plasmid

#165824

Purpose

QF activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

QFAD

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMCS-TCCC

Plasmid

#165774

Purpose

Multiple cloning site part for enhancer cloning, requires minimal promoter element

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

MCS-TCCC

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb

Plasmid

#141067

Purpose

Multisite gateway destination vector for cell free expression (takes two cassettes). Parton lab clone JBO

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Synthetic Biology

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb-EGFP

Plasmid

#118752

Purpose

Multisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEX

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

EGFP

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-ccdb

Plasmid

#118629

Purpose

Multisite gateway destination vector for 5' tagging with mCherry for cell free expression. Parton lab clone FKZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

mCherry

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-ccdb

Plasmid

#118628

Purpose

Multisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

EGFP

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_DL121_WTTS

Plasmid

#171954

Purpose

Selection vector for chimeric fusion of ecDHFR and asLOV2.

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Inserts

Fusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMCRW2

Plasmid

#127380

Purpose

pBC with 4 repeats of 80bp DNA template

Depositor

Richard C. Willson

Article

Chavan et al Analyst. 2020 Jul 21;145(14):4942-4949.

Insert

4 repeats of 80 bp DNA template sequence

Expression

Bacterial

Promoter

lac promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pKP933

Plasmid

#167657

Purpose

Express colicinB-His6, V21C, M261C

Depositor

Kathleen Postle

Article

Skare et al J Bacteriol. 1989 Aug;171(8):4442-7. doi

Insert

cba-his6 V21C, M261C

Expression

Bacterial

Mutation

cba-his6 with V21C, M261C mutations

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMMB206-CT81

Plasmid

#165411

Purpose

Bacteria gene circuit on low copy backbone.

Depositor

Xiaojun Tian

Article

Zhang et al Nat Commun. 2021 Feb 8;12(1):853. doi: 1

Insert

CT81 gene circuit

Expression

Bacterial

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-2

Plasmid

#164859

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-2

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-3

Plasmid

#164860

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-3

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pT7gRNA-ccdb

Plasmid

#140864

Purpose

Backbone for making sgRNAs.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb/CAM

Use

Gateway destination vector

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-639 (attR4-attR2)

Plasmid

#161880

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-624 (attR4-attR2)

Plasmid

#161879

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-HypaCas9-D1135E

Plasmid

#154930

Purpose

aTc inducible HypaCas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

HypaCas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

N692A/M694A/Q695A/H698A/D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-D1135E

Plasmid

#154924

Purpose

aTc inducible Cas9HF1 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

Cas9HF1-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A/D1135E and stop codon on …

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0548

Plasmid

#162299

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-TrxA-[TEV cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEE22

Plasmid

#161787

Purpose

Hi-SC2 reporter delivery plasmid

Depositor

François-Xavier Barre

Article

Espinosa et al Mol Cell. 2020 Sep 3;79(5):857-869.e3. d

Insert

Hi-SC2 reporter

Use

Conjugative plasmid to deliver hi-sc2 reporter

Mutation

Himar site included MmeI site, tandem loxP sites,…

Promoter

n/a

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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