We narrowed to 14,255 results for: TIM

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plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209726

Purpose

Expresses cytosolic medium affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLIC-SM-Myc

Plasmid

#111189

Purpose

High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)

Depositor

Silvia Moreno

Article

Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

Type

Empty backbone

Use

Toxoplasma gondii expression

Tags

Spaghetti Monster Myc Tag

Promoter

Endogenous

Available Since

Aug. 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pET22b-6h-GST-TEVp

Plasmid

#172887

Purpose

Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Insert

TEV protease

Tags

GST solubility tag and HHHHHH

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMM134

Plasmid

#127215

Purpose

BeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, IPT, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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NylCp2-TS_pET28a

Plasmid

#215421

Purpose

Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCp2-TS

Expression

Bacterial

Mutation

D122G/H130Y/D36A/E263Q

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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14UAS PSD95:GFP 5UAS DSRedExpress

Plasmid

#74315

Purpose

Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish

Depositor

Martin Meyer, Stephen Smith

Article

Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

Inserts

zebrafish PSD95
DsRedExpress

Tags

EGFP

Mutation

Y599H and I793S (please see depositor comment bel…

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pET28a-mH6-Cas12i2

Plasmid

#120883

Purpose

Expresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12i2

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

WT

Promoter

lac

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDHt/sk-CE

Plasmid

#92128

Purpose

Expression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Article

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9-eGFP

Use

CRISPR; Trichoderma reesei expression

Tags

EGFP

Promoter

Pcbh2

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymTq2-URA3

Plasmid

#168057

Purpose

Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymTurquoise2

Tags

ymTurquoise2

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pETCON-PTX-S1-220

Plasmid

#67747

Purpose

Pertussis Toxin Subunit 1 Yeast Display Plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

Insert

PTxS1-220

Expression

Bacterial and Yeast

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Integrin alpha5-AHCys

Plasmid

#27313

Depositor

Timothy Springer

Article

Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

Insert

integrin alpha 5 (ITGA5 Human)

Expression

Mammalian

Mutation

integrin alpha5 (1-954) + AHCys

Available Since

March 8, 2011

Availability

Academic Institutions and Nonprofits only
P2-ECFP-pA (Construct 6)

Plasmid

#55198

Purpose

Synthetic promoter responsive to gRNA2

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

mCerulean

Use

Synthetic Biology

Expression

Mammalian

Promoter

Minimal Ade MLP

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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JH01

Plasmid

#73510

Purpose

GFPo_PH pCFJ150 - contains C. elegans sequence optimized GFP

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::GFPo::PH::tbb-2

Tags

GFP

Promoter

mex-5

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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NylCK_pET28a

Plasmid

#215422

Purpose

Expression construct for NylCK gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCK

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_A_1982_F

Plasmid

#237355

Purpose

Expression plasmid for RSV A F from 1982

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV A F from 1982 human codon optimized from Genbank Accesion KJ723472

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_A_2020_F

Plasmid

#237356

Purpose

Expression plasmid for RSV A F from 2020

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV A F from 2020 human codon optimized from Genbank Accesion PP495954

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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LV-PE_CO-Mini

Plasmid

#190338

Purpose

Lentiviral vector expressing PE_CO-Mini (Prime editor with codon-optimized and truncated Reverse transcriptase)

Depositor

Rasmus Bak

Article

Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

Insert

PE_CO-Mini, ngRNA and pegRNA for HEK3 CTTins

Use

Lentiviral

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMM113

Plasmid

#127210

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, AtSTM, sgRNA, Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB179-BhrPETase

Plasmid

#218692

Purpose

Encodes codon optimized BhrPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

BhrPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-DU422gp160

Plasmid

#100926

Purpose

Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (DU422.1) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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